Characterization of a novel high-density lipoprotein antioxidant capacity assay and its application to high-density lipoprotein fractions

Gaál, Krisztina; Lőrincz, Hajnalka; Serés, I.; Harangi, Mariann; Oláh, Anna V.; Paragh, György

doi:10.1016/j.clinbiochem.2013.01.007

Cited by 5 publications

(4 citation statements)

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“…Our method to determine HDL antioxidant capacity has been described previously [ 19 ]. The effect of HDL was measured by heme-catalyzed oxidation of LDL in the absence and presence of HDL.…”

Section: Methodsmentioning

confidence: 99%

“…LDL oxidation was followed spectrophotometrically by measuring hemin degradation. The HDL antioxidant capacity was expressed as the percentage ratio of ΔT Vmax in the presence and absence of HDL [ 19 , 26 ].…”

Section: Methodsmentioning

confidence: 99%

“…Using hemin as a physiological pro-oxidant, we also developed a cell-free method to assess the antioxidant capacity of HDL [ 19 ]. Data were suitable for multiplication with HDL concentration, resulting total HDL antioxidant capacity, which is able to characterize antioxidant attribute and concentration of HDL simultaneously.…”

Section: Introductionmentioning

confidence: 99%

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High-density lipopoprotein antioxidant capacity, subpopulation distribution and paraoxonase-1 activity in patients with systemic lupus erythematosus

et al. 2016

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BackgroundThe causes of increased cardiovascular risk in systemic lupus erythematosus (SLE) are not understood thoroughly, although presence of traditional cardiovascular risk factors and disease-specific agents were also proposed. In this study, we investigated the quantitative changes in the lipid profile, as well as qualitative characteristics of high-density lipoprotein (HDL) and markers of inflammation and disease activity in SLE patients.MethodsLipoprotein levels were determined in 51 SLE patients and 49 healthy controls, matched in age and gender. HDL antioxidant capacity was determined spectrophotometrically with a cell-free method of hemin-induced low-density lipoprotein (LDL) oxidation. Polyacrylamide gel-electrophoresis was used for HDL subfraction analysis. Human paraoxonase-1 (PON1) activity, apolipoprotein A1 (ApoA1) and oxidized LDL concentrations, as well as interleukin-6, high-sensitivity C-reactive protein, serum amyloid A and monocyte chemotactic protein-1 levels were determined.ResultsHDL-cholesterol and ApoA1 concentrations decreased significantly in SLE subjects. Also, PON1 arylesterase activity (125.65 ± 26.87 vs. 148.35 ± 39.34 U/L, p = 0.001) and total HDL antioxidant capacity (165.82 ± 58.28 % vs. 217.71 ± 54.36 %, p < 0.001) were significantly reduced in patients compared to controls. Additionally, all HDL subfraction concentrations were significantly decreased in patients, while the levels of the examined inflammatory markers were significantly elevated in SLE subjects. The latter correlated positively with disease activity, and negatively with HDL concentration and total HDL antioxidant capacity, respectively. PON1 arylesterase activity and erythrocyte sedimentation rate were independent predictors of total HDL antioxidant capacity.ConclusionsInduced by the systemic inflammation, altered composition and antioxidant activity may diminish the anti-atherogenic effect of HDL and therefore may contribute to the increased cardiovascular risk of SLE patients.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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High-density lipopoprotein antioxidant capacity, subpopulation distribution and paraoxonase-1 activity in patients with systemic lupus erythematosus

et al. 2016

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“…Оскільки на сьогодні повний механізм виникнення первинних і вторинних кардіометаболічних ризиків повністю невідомий, а також відсутній уніфікований перелік прогностичних маркерів серцевосудинних захворювань, то вивчення потенційних індикаторів розвитку патологічних станів серцевосудинної системи у пацієнтів з ХХН є необхідним та важливим [6,10,23].…”

Section: Vasylchenkovita@gmailcomunclassified

Activity 0f Lipoprotein-Associated Paraoxonase-1 Enzymes and Myeloperoxidase in Patients with Chronic Kidney Disease

Васильченко¹,

Dunaevskaya²,

Korol³

et al. 2019

Ukr. ž. med. bìol. sportu

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Differences in heme and hemopexin content in lipoproteins from patients with sickle cell disease

Vendrame

Olops

Saad

et al. 2018

Journal of Clinical Lipidology

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Characterization of a novel high-density lipoprotein antioxidant capacity assay and its application to high-density lipoprotein fractions

Cited by 5 publications

References 14 publications

High-density lipopoprotein antioxidant capacity, subpopulation distribution and paraoxonase-1 activity in patients with systemic lupus erythematosus

High-density lipopoprotein antioxidant capacity, subpopulation distribution and paraoxonase-1 activity in patients with systemic lupus erythematosus

Activity 0f Lipoprotein-Associated Paraoxonase-1 Enzymes and Myeloperoxidase in Patients with Chronic Kidney Disease

Differences in heme and hemopexin content in lipoproteins from patients with sickle cell disease

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