Characterization of a Novel Cotton Subtilase Gene GbSBT1 in Response to Extracellular Stimulations and Its Role in Verticillium Resistance

Duan, Xingpeng; Zhang, Zhidong; Wang, Jin; Zuo, Kaijing

doi:10.1371/journal.pone.0153988

Cited by 51 publications

(56 citation statements)

References 37 publications

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“…The corresponding GbSBT1 protein showed to be mainly localized at the cell membrane and moves to the cytoplasm following treatment with jasmonic acid and ethylene, which supports the hypothesis of some grapevine subtilases are located near the place where occurs the plant-pathogen interaction. Moreover, Duan and co-workers, observed a reduction of the tolerance of a cotton resistant genotype, when the GbSBT1 gene was silenced, and an activation of the expression levels of defense-related genes (Duan et al, 2016). …”

Section: Resultsmentioning

confidence: 99%

Revisiting Vitis vinifera Subtilase Gene Family: A Possible Role in Grapevine Resistance against Plasmopara viticola

et al. 2016

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Subtilisin-like proteases, also known as subtilases, are a very diverse family of serine peptidases present in many organisms. In grapevine, there are hints of the involvement of subtilases in defense mechanisms, but their role is not yet understood. The first characterization of the subtilase gene family was performed in 2014. However, simultaneously, the grapevine genome was re-annotated and several sequences were re-annotated or retrieved. We have performed a re-characterization of this family in grapevine and identified 82 genes coding for 97 putative proteins, as result of alternative splicing. All the subtilases identified present the characteristic S8 peptidase domain and the majority of them also have a pro-domain I9 inhibitor, a protease-associated (PA) domain, and a signal peptide for targeting to the secretory pathway. Phylogenetic studies revealed six subtilase groups denominated VvSBT1 to VvSBT6. As several evidences have highlighted the participation of plant subtilases in response to biotic stimulus, we have investigated subtilase participation in grapevine resistance to Plasmopara viticola, the causative agent of downy mildew. Fourteen grapevine subtilases presenting either high homology to P69C from tomato, SBT3.3 from Arabidopsis thaliana or located near the Resistance to P. viticola (RPV) locus were selected. Expression studies were conducted in the grapevine-P. viticola pathosystem with resistant and susceptible cultivars. Our results may indicate that some of grapevine subtilisins are potentially participating in the defense response against this biotrophic oomycete.

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Section: Resultsmentioning

confidence: 99%

Revisiting Vitis vinifera Subtilase Gene Family: A Possible Role in Grapevine Resistance against Plasmopara viticola

et al. 2016

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“…Direct interaction with a pathogen effector was shown for GbSBT1 from cotton ( Gossypium barbadense ). The protease was shown to interact with a prohibitin (PHB)‐like effector protein which is secreted by Verticillium dahliae during infection (Duan et al ., ). Gain‐ and loss‐of‐function studies confirmed a role for GbSBT1 in Verticillium wilt resistance (Duan et al ., ).…”

Section: Physiological Roles Of Plant Sbtsmentioning

confidence: 97%

From structure to function – a family portrait of plant subtilases

et al. 2017

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Contents Summary901I.Introduction901II.Biochemistry and structure of plant SBTs902III.Phylogeny of plant SBTs and family organization903IV.Physiological roles of plant SBTs905V.Conclusions and outlook911Acknowledgements912References912 Summary Subtilases (SBTs) are serine peptidases that are found in all three domains of life. As compared with homologs in other Eucarya, plant SBTs are more closely related to archaeal and bacterial SBTs, with which they share many biochemical and structural features. However, in the course of evolution, functional diversification led to the acquisition of novel, plant‐specific functions, resulting in the present‐day complexity of the plant SBT family. SBTs are much more numerous in plants than in any other organism, and include enzymes involved in general proteolysis as well as highly specific processing proteases. Most SBTs are targeted to the cell wall, where they contribute to the control of growth and development by regulating the properties of the cell wall and the activity of extracellular signaling molecules. Plant SBTs affect all stages of the life cycle as they contribute to embryogenesis, seed development and germination, cuticle formation and epidermal patterning, vascular development, programmed cell death, organ abscission, senescence, and plant responses to their biotic and abiotic environments. In this article we provide a comprehensive picture of SBT structure and function in plants.

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“…In the past year, several studies have highlighted the involvement of subtilases in plant resistance to pathogens. In cotton, a subtilase called GbSBT1 has been identified, characterized and related to Verticillium dahliae ‐induced resistance (Duan et al ., ). In this study, the authors observed that GbSBT1 knockdown reduces cotton defence against V. dahliae attack.…”

Section: Subtilases Involved In Plant Defencementioning

confidence: 97%

Subtilisin‐like proteases in plant defence: the past, the present and beyond

Figueiredo

Silva

Figueiredo

2017

Molecular Plant Pathology

111

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Subtilisin-like proteases (or subtilases) are a very diverse family of serine peptidases present in many organisms, but mostly in plants. With a broad spectrum of biological functions, ranging from protein turnover and plant development to interactions with the environment, subtilases have been gaining increasing attention with regard to their involvement in plant defence responses against the most diverse pathogens. Over the last 5 years, the number of published studies associating plant subtilases with pathogen resistance and plant immunity has increased tremendously. In addition, the observation of subtilases and serine protease inhibitors secreted by pathogens has also gained prominence. In this review, we focus on the active participation of subtilases in the interactions established by plants with the environment, highlighting their role in plant-pathogen communication.

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Characterization of a Novel Cotton Subtilase Gene GbSBT1 in Response to Extracellular Stimulations and Its Role in Verticillium Resistance

Cited by 51 publications

References 37 publications

Revisiting Vitis vinifera Subtilase Gene Family: A Possible Role in Grapevine Resistance against Plasmopara viticola

Revisiting Vitis vinifera Subtilase Gene Family: A Possible Role in Grapevine Resistance against Plasmopara viticola

From structure to function – a family portrait of plant subtilases

Subtilisin‐like proteases in plant defence: the past, the present and beyond

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