Characterization of a novel CC chemokine CCL4 in immune response induced by nitrite and its expression differences among three populations of Megalobrama amblycephala

Zhang, Jie; Chen, Liping; Wei, Xinlan; Xu, Mengxia; Huang, Chen; Wang, Weimin; Wang, Huanling

doi:10.1016/j.fsi.2014.02.012

Cited by 15 publications

(7 citation statements)

References 46 publications

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“…But the high expression of CCL4 in the liver, kidney and skin at 24 hr post‐vaccination indicates the capability of LAVh to induce innate immune response in internal organs as well as in mucosal organs, such as the skin. Similarly, previous research has shown the liver and kidney of Wuchang bream fish, Megalobrama amblycephala tend to show a high expression of CCL4 (Zhang, Chen, et al, ). It is believed that live LAVh is more effective in transferring the lipopolysaccharide to leucocytes and macrophages via phagocytosis in the liver, kidney and skin compared with the formalin‐killed V. harveyi .…”

Section: Discussionsupporting

confidence: 57%

Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer

et al. 2019

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Vibrio harveyi causes vibriosis in various marine aquaculture fish species, especially when they are young. The infection subsequently leads to significant economic losses for aquaculture farms. Vaccination is recommended to control this disease. This study describes the efficacy of a live attenuated V. harveyi strain MVh_vhs (LAVh) as a vaccine candidate in controlling infection by wild‐type V. harveyi (WTVh) in Lates calcarifer. A total of 240 fingerlings were divided into four groups. Group 1 was not vaccinated and was not challenged, Group 2 was vaccinated with a formalin‐killed V. harveyi (FKVh), Group 3 was vaccinated with the LAVh before challenge and Group 4 was not vaccinated and was challenged. Bath vaccination was employed for one hour before the LAVh distribution was determined in the fish mucus, gill, liver, gut, kidney and spleen. The gills, livers, kidneys and skins were also sampled for gene expression analysis. To challenge the fish, skin abrasion was conducted before the fish were challenged by immersion with WTVh. The results revealed an extensive distribution of the LAVh in the liver and kidneys of the fish in Group 3 for the first 12 hr, resulting in mild lesions compared with Group 1. Similarly, there were significantly (p < .05) higher expressions of the Chemokine ligand 4 and major histocompatibility complex I genes in the skin and liver of the fish in Group 3 in comparison with other groups. Vaccination with LAVh resulted in a significantly high rate of survival (68%) of the fingerlings after being challenged with WTVh.

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Section: Discussionsupporting

confidence: 57%

Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer

et al. 2019

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“…These proteins are suggested as “stress sensors”, all involved in growth suppression, cell-cycle arrest and apoptosis 45–47 . Lower levels of expression of different immune response components were also detected in the PH individuals, including various members of the chemokine (C-C motif) ligand, CCL4 , CCL19 , CCL20 and CCL28 all playing important roles in both innate and adaptive immunity 48–50 .…”

Section: Resultsmentioning

confidence: 99%

Impacts of Early Life Stress on the Methylome and Transcriptome of Atlantic Salmon

Moghadam

Robinson

Andersen

et al. 2017

Sci Rep

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Exposure to environmental stressors during early-life stages can change the rate and timing of various developmental processes. Epigenetic marks affecting transcriptional regulation can be altered by such environmental stimuli. To assess how stress might affect the methylome and transcriptome in salmon, fish were treated using cold-shock and air-exposure from the eye-stage until start-feeding. The fish were either stressed prior to hatching (E), post-hatching (PH), pre- and post-hatching (EPH) or not stressed (CO). Assessing transcriptional abundances just prior to start feeding, E and PH individuals were found to have modified the expression of thousands of genes, many with important functions in developmental processes. The EPH individuals however, showed expression similar to those of CO, suggesting an adaptive response to extended periods of stress. The methylome of stressed individuals differed from that of the CO, suggesting the importance of environment in shaping methylation signatures. Through integration of methylation with transcription, we identified bases with potential regulatory functions, some 10s of kb away from the targeted genes. We then followed fish growth for an additional year. Individuals in EPH showed superior growth compared to other treatment groups, highlighting how stress can potentially have long-lasting effects on an organism’s ability to adapt to environmental perturbations.

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“…Both cytokines and their receptors can be grouped by structure into different families. Of the downregulated genes in the cytokine-cytokine receptor interaction pathway (figure 5), the subfamily CC chemokines receptor ccr6, ccr7 and ccr9 and the ligand ccl4 can be found, which are critical for the leucocyte migration during inflammation [54,55], and activation of the secondary immune response [56]. Interestingly, also in human clinical trials, using a ccr9 antagonist to reduce ccr9 gene expression has been modelled as an effective treatment in preventing inflammatory bowel disease, a chronic inflammation of the gastrointestinal tract that causes long-term tissue damages and often irreversible impairment of the structure and functions [57].…”

Section: Discussionmentioning

confidence: 99%

Mid and hindgut transcriptome profiling analysis of Atlantic salmon ( Salmon salar ) under unpredictable chronic stress

et al. 2020

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The intestinal epithelium is a selectively permeable barrier for nutrients, electrolytes and water, while maintaining effective protection against pathogens. Combinations of stressors throughout an animal's life, especially in agriculture and aquaculture settings, may affect the regular operativity of this organ with negative consequences for animal welfare. In the current study, we report the effects of a three-week unpredictable chronic stress (UCS) period on the intestinal morphology and transcriptome response of Atlantic salmon ( Salmon salar ) parr midgut and hindgut. Midgut and hindgut from both control and UCS fish were collected for histology and RNA-sequencing analysis to identify respective changes in the membrane structures and putative genes and pathways responding to UCS. Histological analysis did not show any significant effect on morphometric parameters. In the midgut, 1030 genes were differentially expressed following UCS, resulting in 279 genes which were involved in 13 metabolic pathways, including tissue repair pathways. In the hindgut, following UCS, 591 differentially expressed genes were detected with 426 downregulated and 165 upregulated. A total of 53 genes were related to three pathways. Downregulated genes include cellular senescence pathways, p53 signalling and cytokine–cytokine receptor pathways. The overall results corroborate that salmon parr were at least partly habituating to the UCS treatment. In midgut, the main upregulation was related to cell growth and repair, while in the hindgut there were indications of the activated apoptotic pathway, reduced cell repair and inhibited immune/anti-inflammatory capacity. This may be the trade-off between habituating to UCS and health resilience. This study suggests possible integrated genetic regulatory mechanisms that are tuned when farmed Atlantic salmon parr attempt to cope with UCS.

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Characterization of a novel CC chemokine CCL4 in immune response induced by nitrite and its expression differences among three populations of Megalobrama amblycephala

Cited by 15 publications

References 46 publications

Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer

Efficacy of bath vaccination with a live attenuated Vibrio harveyi against vibriosis in Asian seabass fingerling, Lates calcarifer

Impacts of Early Life Stress on the Methylome and Transcriptome of Atlantic Salmon

Mid and hindgut transcriptome profiling analysis of Atlantic salmon ( Salmon salar ) under unpredictable chronic stress

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