Characterization of a novel catalase gene and its response to low temperature stress in noble scallop Chlamys nobilis with different total carotenoids content

Abstract

Huge temperature fluctuation has great deleterious effects on marine invertebrates (Hennig & Andreatta, 1998). Under extreme conditions, DNA, lipids and proteins would be damaged by excessive reactive oxygen species (ROS) (Evans & Halliwell, 1999). To maintain the redox balance, marine invertebrates have evolved a very complex antioxidant system to eliminate ROS, which includes many antioxidants enzymes and non-enzymatic antioxidants, for example catalase (CAT), superoxide dismutase (SOD) and carotenoids (Abele

Molecular Cloning and Functional Characterization of Catalase in Stress Physiology, Innate Immunity, Testicular Development, Metamorphosis, and Cryopreserved Sperm of Pacific Abalone

“…In the present study, induced CAT activity and higher Hdh-CAT mRNA expression levels were observed in response to thermal stress. Similar findings have been previously reported for CAT in scallop [4], ark shell [21], Mediterranean mussel [60], and Pacific oyster [61]. The present study also found elevated ROS levels in thermal-stressed abalone, consistent with previous findings of American oyster [31].…”

“…Present findings suggest that Hdh-CAT might play a role in the antioxidant defensive mechanism against ROS generation induced by H 2 O 2 . Another antioxidant, SOD, has been previously recommended as an antioxidant defender against H 2 O 2 -induced damage in Pacific abalone [4].…”

“…Catalase (CAT) is the most frequently used biomarker of oxidative stress in animals of aquatic environments [1,2]. Catalase is a vital antioxidant enzyme that can balance the redox system by regulating antioxidant defenses against oxidative stress [3,4]. This antioxidant enzyme responds to the adverse effects of environmental pollution on organisms [1].…”

“…Heat stress also induces CAT activity and increases its mRNA expression in a marine mollusk, Scapharca subcrenata [21]. Cold stress imbalances CAT activity and expression in scallop, Chlamys nobilis [4]. CAT neutralizes the effects of ROS production during starvation [22].…”