Characterization of a novel Aspergillus niger beta-glucosidase tolerant to saccharification of lignocellulosic biomass products and fermentation inhibitors

Oriente, Alesandra; Tramontina, Robson; Andrades, Diandra de; Henn, Caroline; Silva, Jose L. C.; Simão, Rita de Cássia Garcia; Maller, Alexandre; Polizeli, Maria de Lourdes Teixeira de Moraes; Kadowaki, Marina Kimiko

doi:10.1515/chempap-2015-0111

Cited by 18 publications

(4 citation statements)

References 15 publications

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“…A study on Prunus armeniaca has also shown that the β -glucosidase exhibited higher activities under low pH levels (4 to 6), and the optimum temperature for the activity of this enzyme was 35°C ( Bhalla et al, 2017 ). Oriente et al (2015) also reported that the optimum pH for β -glucosidase activity was 4.0 although it was stable in the range of 4.0–7.0. Therefore, the environmental conditions of the ensiling ratooning sorghum, lower pH levels in silage and the suitable temperature of fall, might be helpful to remove the CNglcs, and finally resulting in a safe HCN content for livestock.…”

Section: Discussionmentioning

confidence: 98%

Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum

Zhai

Wang²,

Sun³

et al. 2023

Front. Microbiol.

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IntroductionCyanogenic glycosides (CNglcs) are bioactive plant products involving in plant defense against herbivores by virtue of their abilities to release toxic hydrogen cyanide (HCN). Aspergillus niger has been shown to be effective in producing β-glucosidase, which could degrade CNglcs. However, whether A. niger could remove CNglcs under ensiling conditions is still unknown.MethodsIn this study, we first investigated the HCN contents in ratooning sorghums for two years, then the sorghums were ensiled with or without the addition of A. niger.ResultsTwo years’ investigation indicated that the contents of HCN in fresh ratooning sorghum were larger than 801 mg/kg FW (fresh weight), which could not be reduced by silage fermentation under safety threshold (200 mg/kg FW). A. niger could produce β-glucosidase over a range of pH and temperature, which degraded the CNglcs and removed the hydrogen cyanide (HCN) at early days of ratooning sorghum fermentation. The addition of A. niger (2.56 × 107 CFU/ml) altered the microbial community, increased bacterial diversity, improved the nutritive qualities, and reduced the HCN contents in ensiled ratooning sorghum lower than 100 mg/kg FW after 60 days of fermentation. Overall, the addition of 150 ml A. niger + 50 ml sterile water per 3 kg silage could efficiently remove CNglcs from ratooning sorghum silage.ConclusionIn conclusion, A. niger could produce β-glucosidase which degraded the CNglcs during the early days of fermentation, benefiting the ensiling process and improving the utilization of ratooning sorghum.

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Section: Discussionmentioning

confidence: 98%

Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum

Zhai

Wang²,

Sun³

et al. 2023

Front. Microbiol.

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“…Strain identification was based on the isolation of the genomic DNA of the fungus [16] by analysis of the amplified products of the Internal Transcribed Spacer (ITS) regions of the extracted ribosomal DNA of the fungus [17], and the ITS sequence was deposited in the GenBank database under accession number KJ470626. The phylogenetic tree was built from sequences of regions of A. flavus and compared with sequences of other A. flavus fungal strains obtained from the NCBI database with the assistance of the Mega X Molecular Evolutionary Genetics Analysis) software [18] applying the Maximum Likelihood method and Jukes-Cantor model [19].…”

Section: Isolation and Strain Identificationmentioning

confidence: 99%

Thermoacidophilic β-fructofuranosidase induced with soybean meal produced by a new Aspergillus flavus isolated from the Atlantic Forest

De Andrades,

Inagaki,

Lopez Karg

et al. 2024

Sci. Plena

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High production of β-fructofuranosidase was obtained with the new fungus Aspergillus flavus isolated from the Atlantic Forest under solid state fermentation supplemented with soybean meal (53.36 U/ml). This beta-fructofuranosidase obtained after six days of cultivation with soybean meal was partially purified by DEAE-Sephadex ion exchange chromatographic columns, with a final yield of 19%, with an apparent molecular mass of 37 KDa by SDS-PAGE. The optimum pH and temperature for enzyme activity were 5.0 and 60 °C, respectively. The enzyme showed stability with 70% residual activity after 12 hours in the acid pH range (5.0 and 5.5), while at temperatures from 45 ºC to 55 ºC the enzyme showed stability greater than 50%. Therefore, the characteristics of this thermoacidophilic β-fructofuranosidase from A. flavus presents potential for use in several biotechnological processes.

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“…Fungi from the section Nigri are known to produce xylanases and β-glucosidases [46,47]. So, A. brasiliensis was added to the cocktail to supplement the cellulolytic cocktail secreted by T. reesei.…”

Section: Enzymatic Activity Pro Lementioning

confidence: 99%

Co-Culture of Trichoderma Reesei, Talaromyces Sp. and Aspergillus Spp. Produces A Multi-Enzyme Cocktail for the Hydrolysis of Sugarcane Bagasse Pretreated with Piperonilic Acid (PIP) and Methylenedioxycinnamic Acid (MDCA)

Silva

Oliveira

Lima

et al. 2022

Preprint

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PURPOSE: The global energy matrix is primarily based on fossil fuels and alternatives for the production of renewable energy are necessary. The second-generation ethanol (2G ethanol) is such alternative. 2G ethanol is produced through the fermentation of sugar released from the enzymatic hydrolysis of lignocellulosic biomass. However, this process is still costly. Improvements should include the use of less expensive biomass pre-treatments and enzymatic cocktails produced by the co-cultivation of filamentous fungi. METHODS: For the production of synergistic holocellulolytic enzymes, Aspergillus brasiliensis , A. fumigatus var. niveus , Trichoderma reesei and Talaromyces sp. were co-cultivated on sugarcane bagasse modified in the lignin synthesis pathway. This bagasse was pretreated with piperonilic acid (PIP) and methylenedioxycinnamic acid (MDCA). RESULTS: The enzymatic cocktail produced by the co-culture showed the highest hydrolysis efficiency. The best hydrolysis condition was at 50°C and pH 4.0. Talaromyces sp. and T. reesei demonstrated antagonism only between them. CONCLUSION: Enzymatic cocktails produced through the co-cultivation of filamentous fungi are a concrete step towards increasing yields for the 2G ethanol industry.

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Characterization of a novel Aspergillus niger beta-glucosidase tolerant to saccharification of lignocellulosic biomass products and fermentation inhibitors

Cited by 18 publications

References 15 publications

Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum

Effects of Aspergillus niger on cyanogenic glycosides removal and fermentation qualities of ratooning sorghum

Thermoacidophilic β-fructofuranosidase induced with soybean meal produced by a new Aspergillus flavus isolated from the Atlantic Forest

Co-Culture of Trichoderma Reesei, Talaromyces Sp. and Aspergillus Spp. Produces A Multi-Enzyme Cocktail for the Hydrolysis of Sugarcane Bagasse Pretreated with Piperonilic Acid (PIP) and Methylenedioxycinnamic Acid (MDCA)

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