Characterization of a new low molecular weight RNA in HeLa cell ribosomes

Pène, Jacques J.; Knight, Ernest; Darnell, James

doi:10.1016/0022-2836(68)90309-4

Cited by 299 publications

(90 citation statements)

References 17 publications

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“…3 d ) at intermediate temperatures reflects a conformational change within the molecule, or the further dissociation of a small fragment as has been found in other eukaryotes (Pene, Knight & Darnell, 1968;Stevens & Pachler, 1972) is uncertain, since the occurrence of low molecular weight RNAs after denaturation of L-RNA was not investigated.…”

Section: Discussionmentioning

confidence: 99%

Lability of RNA from the Large Cytoplasmic Ribosomal Subunit of the Protozoon Crithidia oncopelti

Spencer¹,

Cross²

1976

Journal of General Microbiology

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Section: Discussionmentioning

confidence: 99%

Lability of RNA from the Large Cytoplasmic Ribosomal Subunit of the Protozoon Crithidia oncopelti

Spencer¹,

Cross²

1976

Journal of General Microbiology

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“…They are apparently dissimilar in that the bacterial 5S rRNA cistron is included in the transscriptional unit, while the mammalian 5S rRNA gene is excluded. However, a fourth mammalian rRNA, 7S rRNA, is transcribed from the same unit as 18S and 28S rRNA, and remains covalently bonded to the latter species during early stages of ribosome maturation (2). It is possible that mammalian 7S rRNA, and not 5S rRNA, is the evolutionary homolog of bacterial 5S rRNA.…”

Section: Discussionmentioning

confidence: 99%

“…In eukaryotic cells, linked 18S, 28S, and 7S ribosomal RNA cistrons are transcribed as a unit into a single polynucleotide "precursor" (45S rRNA), which is ultimately cleaved to produce one molecule of each of the three rRNA species (1,2). The three linked cistrons together comprise a single "transcriptional unit", which we define here as a segment of DNA bounded by a single site for RNA polymerase attachment (promoter) and a single site for polymerase release (terminator).…”

mentioning

confidence: 99%

Transcriptional Organization of the Ribosomal RNA Cistrons in Escherichia Coli

Doolittle

Pace

1971

Proc. Natl. Acad. Sci. U.S.A.

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The data presented support the hypothesis that 16S, 23S, and 5S ribosomal RNAs of Escherichia coli are transcribed in vivo from transcriptional units consisting of single cistrons for these species arranged in the order 16S-23S-5S, with transcription beginning at the 16S end.In eukaryotic cells, linked 18S, 28S, and 7S ribosomal RNA cistrons are transcribed as a unit into a single polynucleotide "precursor" (45S rRNA), which is ultimately cleaved to produce one molecule of each of the three rRNA species (1, 2). The three linked cistrons together comprise a single "transcriptional unit", which we define here as a segment of DNA bounded by a single site for RNA polymerase attachment (promoter) and a single site for polymerase release (terminator). Many such rRNA transcriptional units are confined to a restricted region of the eukaryotic chromosome; adjacent units may be separated by a segment of (nonribosomal) "spacer" DNA (1).The genetic organization of the cistrons for bacterial rRNA species (16S, 23S, and 5S rRNA) is similar to that of eukaryotes in that: (a) the number of gene copies (per genome) for each RNA species is the same (3, 4), (b) these cistrons are confined to one (5) or possibly two (6, 7) small regions of the genome, (c) 16S, 23S, and 5S rRNA cistrons are tightly linked (8, 9), and (d) adjacent 16S, 23S, and 5S gene clusters are separated from each other by segments of spacer DNA (10).Data presented here support the hypothesis that the linked 16S, 23S, and 5S rRNA cistrons in Escherichia coli comprise single transcriptional units arranged in the order 16S-23S-5S, with transcription initiating at the 16S end. The fact that no "precursor" comparable to eukaryotic 45S rRNA has yet been found in bacteria may imply that cleavage into 16S and 23S (and possibly 5S) moieties occurs before transcription of the unit is complete. MATERIALS AND METHODSFor the preparation of intact 3H-labeled RNA from rifampicininhibited cells, a culture of the rifampicin-sensitive, E. coli B strain AS19 (11) Corp.), was chased by the addition of excess unlabeled orthophosphate, and after 30 min of further growth at 200C, was chilled to 00C in ice. Rifampicin (Pittman-Moore) was added to 200 Ag/ml at 2 min and [5-3H]uridine (New England Nuclear Corp.) was added to 0.04 mCi/ml (10 ;&g/ml) at 4 min after chilling. At 6 min the culture was warmed to 20'C and incubation was continued for an additional 40 min. Cultures were harvested by centrifugation and RNA was extracted and resolved on 2.8% polyacrylamide gels (13,14).For the preparation of RNA for oligonucleotide mapping,

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“…5.8 S rRNA is a low-molecular-weight RNA species that occurs in the large ribosomal subunit of eukaryotes (Pene et al, 1968) but not prokaryotes (Erdmann, 1976). The nucleotide sequences of the 5.8S rRNA of several eukaryotes are known (Rubin, 1973;Nazar et al, 1975bHampe et al, 1976;Nazar & Roy, 1976).…”

mentioning

confidence: 99%

“…Further evidence on the conformation of 5.8 S rRNA would be of interest for various reasons. For example, 5.8S rRNA appears to exist in the ribosome in the form of a specific complex with 28S rRNA, whose integrity survives extraction with phenol at 20°C (Pene et al, 1968;King & Gould, 1970). Therefore the complex must be at least partly maintained by RNA-RNA interactions.…”

mentioning

confidence: 99%

Evidence on the conformation of HeLa-cell 5.8S ribosomal ribonucleic acid from the reaction of specific cytidine residues with sodium bisulphite

Kelly¹,

Goddard²,

Maden³

1978

Biochemical Journal

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The reaction of HeLa-cell 5.8 S rRNA with NaHSO3 under conditions in which exposed cytidine residues are deaminated to uridine was studied. It was possible to estimate the reactivities of most of the 46 cytidine residues in the nucleotide sequence by comparing 'fingerprints' of the bisulphite-treated RNA with those of untreated RNA. The findings were consistent with the main features of the secondary-structure model for mammalian 5.8 S rRNA proposed by Nazar, Sitz & Busch [J. Biol. Chem. (1975) 250,[8591][8592][8593][8594][8595][8596][8597]. Five out of six regions that are depicted in the model as single-stranded loops contain cytidine residues that are reactive towards bisulphite at 25°C (the other loop contains no cytidine). The cytidine residue nearest to the 3'-terminus is also reactive. Several cytidine residues that are internally located within proposed double-helical regions show little or no reactivity towards bisulphite, but the cytidine residues of several C. G pairs at the ends of helical regions show some reactivity, and one of the proposed loops appears to contain six nucleotides, rather than the minimum of four suggested by the primary structure. Two cytidine residues that are thought to be 'looped out' by small helix imperfections also show some reactivity.

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Characterization of a new low molecular weight RNA in HeLa cell ribosomes

Cited by 299 publications

References 17 publications

Lability of RNA from the Large Cytoplasmic Ribosomal Subunit of the Protozoon Crithidia oncopelti

Lability of RNA from the Large Cytoplasmic Ribosomal Subunit of the Protozoon Crithidia oncopelti

Transcriptional Organization of the Ribosomal RNA Cistrons in Escherichia Coli

Evidence on the conformation of HeLa-cell 5.8S ribosomal ribonucleic acid from the reaction of specific cytidine residues with sodium bisulphite

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