Characterization of a new DRB4 allele (DRB4*0104)

Canck, I. De; Demanet, Christian; Mersch, G.; Jannes, Geert; Rombout, A; Rossau, R.; Waeyenberge, C Van

doi:10.1111/j.1399-0039.1996.tb02632.x

Cited by 8 publications

(1 citation statement)

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“…The exon 2 sequence of the 5' polymorphic region of DRB4*0102 up to codon 21 was missing. Therefore reversed sequencing was performed after amplification with a specific 3' primer (DRB96034) in combination with a generic 5' primer (DRB96142b) located in intron 1 (19). DRB96032c was used as internal sequencing primer.…”

Section: ' Orbmentioning

confidence: 99%

High‐resolution HLA typing for the DRB3/4/5 genes by sequence‐based typing

1997

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The high degree of polymorphism of the HLA genes at the nucleotide sequence level has proven sequence-based typing a major typing strategy. For DRB1 the allelic variability is predominantly present in the second exon and by DNA sequencing of exon 2 all hitherto known DRB1 alleles can be detected. For the associated genes DRB3, DRB4 and DRB5 the situation is slightly different. Allelic differences are not limited to exon 2 and the sequence of exon 3 and sometimes exon 4 is needed for complete subtyping. Oligonucleotides to amplify the exons needed for subtyping of DRB3, DRB4 and DRB5 were designed. Gene-specific products were generated to make simultaneous detection of alleles in heterozygous combinations possible. In this way 238 individuals were fully typed for their DRB3, 4 and 5 subtypes. Additional samples were typed for only one of the genes. All samples had been previously typed by PCR-SSP. Concordant typing results were obtained for all individuals tested. The DRB3 alleles typed for included *0101, *0201, *0202 and *0301, for DRB4 they were *01011, *0102 and *0103 and for DRB5 *0101, *0102, *0103, *0105, *0201, *0202 and *0203. All alleles were easily detected by the protocol described except for DRB5*0201. Sequencing of exon 3 and 4 of the DRB5*0201 allele showed this allele to be a sequencing error and the sequences obtained were identical to the exon 2, 3 and 4 sequences of DRB5*0202. Two new alleles were identified in the samples studied, DRB4*0105 and DRB3*0207. Sequence based typing has been recognized as a valuable tool for HLA typing of DRB1, DQB1 and DPB1 since several years. It is shown to be a superior typing method as well in the detection of the different DRB3, 4 and 5 subtypes.

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Section: ' Orbmentioning

confidence: 99%