Characterization of a microfluidic magnetic bead separator for high-throughput applications

Bu, Minqiang; Christensen, Troels Balmer; Smistrup, Kristian; Wolff, Anders; Hansen, Mikkel Fougt

doi:10.1016/j.sna.2007.12.014

Cited by 51 publications

(36 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This system consists of an array of integrated soft-magnetic elements embedded in a nonmagnetic substrate beneath a microfluidic channel. 11 A bias field is used to magnetize the elements that produce a field gradient that imparts a force to magnetic particles within the microchannel. We study this system using a Newtonian transport model.…”

Section: Passive Magnetophoretic Bioseparationmentioning

confidence: 99%

“…8 In its most basic form, a magnetophoretic microsystem consists of a microfluidic channel and embedded elements that produce a magnetic field distribution within the microchannel. The elements can be either passive magnetic structures 11 (Figure 6.3) or active voltage-driven conductors 12 (Figure 6.4). In either case, the field generated by the elements gives rise to a magnetic force that acts to manipulate, immobilize, sort, or trap magnetically labeled material as it flows through the microchannel.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Particle Transport in Magnetophoretic Microsystems

Furlani

2010

Microfluidic Devices in Nanotechnology

View full text Add to dashboard Cite

We present methods and models for predicting the transport and trapping of magnetic particles in microfluidic systems with magnetic functionality. We discuss particle transport models that take into account the dominant magnetic and fluidic forces, as well as Brownian motion for sufficiently small particles. We use the transport models to study the performance of magnetically-biased and electrically actuated microsystems.

show abstract

Section: Passive Magnetophoretic Bioseparationmentioning

confidence: 99%

mentioning

confidence: 99%

Particle Transport in Magnetophoretic Microsystems

Furlani

2010

Microfluidic Devices in Nanotechnology

View full text Add to dashboard Cite

show abstract

“…In the past few years, several microfluidic based magnetic sorting concepts have been investigated to capture magnetic beads or magnetically labeled cells. [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] However, these devices are generally hampered by complex fabrication processes and low volumetric throughputs. In many earlier studies, flow rates were limited to less than 1 ml/h, 9,10 which are not practical for many realworld applications.…”

Section: Introductionmentioning

confidence: 99%

On-chip magnetophoretic isolation of CD4 + T cells from blood

Darabi

Guo

2013

Biomicrofluidics

View full text Add to dashboard Cite

This paper presents the design, fabrication, and testing of a magnetophoretic bioseparation chip for the rapid isolation and concentration of CD4 þ T cells from the peripheral blood. In a departure from conventional magnetic separation techniques, this microfluidic-based bioseperation device has several unique features, including locally engineered magnetic field gradients and a continuous flow with a buffer switching scheme to improve the performance of the separation process. Additionally, the chip is capable of processing significantly smaller sample volumes than conventional methods and sample losses are eliminated due to decreased handling. Furthermore, the possibility of sample-to-sample contamination is reduced with the disposable format. The overall dimensions of the device were 22 mm by 60 mm by 1 mm, approximately the size of a standard microscope slide. The results indicate a cell purity of greater than 95% at a sample flow rate of 50 ml/h and a cell recovery of 81% at a sample flow rate of 10 ml/h. The cell purity was found to increase with increasing the sample flow rate. However, the cell recovery decreases with an increase in the flow rate. A parametric study was also performed to investigate the effects of channel height, substrate thickness, magnetic bead size, and number of beads per cell on the cell separation performance. V C 2013 AIP Publishing LLC. [http://dx

show abstract

“…Pamme and Manz (2004) developed a microfluidic chip system which would be beneficial not only to separate magnetic particles from nonmagnetic material but also to separate magnetic particles with different properties from each other. Bu et al (2008) presented an efficient magnetic (4) micropillars with a gap of 2.5 lm; (5) lateral stream channels for blood plasma bead separator based on an external checkerboard array of permanent magnets providing long-range magnetic capturing forces combined with on-chip permalloy elements providing strong short-range magnetic retaining forces. Although this device wasn't applied to real sample, it has provided a new idea to capture the special beads or cells.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic devices for sample pretreatment and applications

Xing

Cui

2009

Microsyst Technol

View full text Add to dashboard Cite

With the fundamentals of microscale flow and species transport well developed, the recent trend in microfluidics has been to work towards the devices capable of dealing with 'real' crude samples directly. Before the real samples reaching the analytical step, it nearly requires significant pre-treated steps. Development of these microdevices that perform sample preprocessing steps is now underway for a broad range of application areas from onchip DNA analysis, immunoassays to cytometry. This article provides an overview of the latest developments in microfluidic devices for sample pretreatment. Many of the microchips are being designed for individual preprocessing steps, but integration of multiple sample preparation steps has been shown, along with integration of sample preprocessing and analytical procedures on single microchips.

show abstract

Characterization of a microfluidic magnetic bead separator for high-throughput applications

Cited by 51 publications

References 29 publications

Particle Transport in Magnetophoretic Microsystems

Particle Transport in Magnetophoretic Microsystems

On-chip magnetophoretic isolation of CD4 + T cells from blood

Microfluidic devices for sample pretreatment and applications

Contact Info

Product

Resources

About