Characterization of a lipid-rich fraction synthesized by streptomyces avermitilis

Metz, Peter; Omstead, Daniel R.; Kaplan, Louis; Liesch, Jerrold M.; Stearns, Ralph A.; Vandenheuvel, W.J.A.

doi:10.1016/s0021-9673(01)84652-5

Cited by 9 publications

(8 citation statements)

References 16 publications

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“…(Clejan et al 1986), Aquaspirillum magnetotacticum (Gorby et al 1988), Streptomyces hygroscopicus (Hoischen et al 1997) and Streptomyces avermitilis (Metz et al 1988). However, these data are very scarce except for the strain S. avermitilis, where FA chain length (C 15 -C 17 ) was determined and the strain Streptomyces sp.…”

Section: Discussionmentioning

confidence: 99%

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Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp. NP10

Ilić-Tomič

Genčić

Živković

et al. 2015

Appl Microbiol Biotechnol

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Herein, a novel soil bacterium Streptomyces sp. NP10 able to grow outside usual streptomycetes optimum conditions (e.g., at 4 °C, pH 9 and high NaCl concentration), exhibiting atypical hemolytic, DNAse, and cellulolytic activities, is described. This strain produces and excretes into the growth medium large amounts of free long-chain fatty acids (FAs). A concurrent lipidomics study revealed a large structural diversity of FAs with over 50 different n- and branched-chain, (un)saturated, and cyclopropane FAs (C7-C30) produced by this strain. Two of these, i-17:0cy9-10 and a-18:0cy9-10, represent new natural products and the first ever identified branched cyclopropane FAs. Both free and bound lipid profiles of Streptomyces sp. NP10 were dominated by saturated branched chain FAs (i-14:0, a-15:0, and i-16:0). Although these free FAs showed only a moderate antimicrobial activity, our results suggest that they could have an ecophysiological role in interspecies signaling with another soil microorganism Pseudomonas aeruginosa. This work represents the first comprehensive report on the structural diversity and complexity of the free FA pool in Streptomyces. A naturally occurring streptomycete, such as Streptomyces sp. NP10, which secretes significant amounts of free long-chain FAs (non-cytotoxic) into the medium, could be useful in microbial biodiesel production.

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Section: Discussionmentioning

confidence: 99%

“…(Metz et al 1988;Hoischen et al 1997) led us to examine if this increased production and secretion of free FAs is a unique feature of the particular strain Streptomyces sp. NP10 or it is perhaps a characteristic of all members of Streptomyces genus previously unpublished.…”

Section: Taxonomic Significance Of Free Fasmentioning

confidence: 99%

Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp. NP10

Ilić-Tomič

Genčić

Živković

et al. 2015

Appl Microbiol Biotechnol

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“…The lipid fraction synthesized by S. avermitilis consists primarily of a mixture of triglycerides possessing C 14 ‐C 17 acyl group and low levels of C 15 ‐C 17 free fatty acids, squalene and diglycerides (Metz et al . ). The fatty acid triglycerides cannot be used as fuel directly and require slight chemical modification mainly transesterification, pyrolysis and emulsification.…”

Section: Resultsmentioning

confidence: 97%

“…In Streptomyces as well as in other actinobacteria, de novo synthesized fatty acids using type II fatty acid synthase system are not only dedicated to building membrane phospholipids but also are incorporated into neutral lipid storage compounds (triacylglycerides) (Arabolaza et al 2010). The lipid fraction synthesized by S. avermitilis consists primarily of a mixture of triglycerides possessing C 14 -C 17 acyl group and low levels of C 15 -C 17 free fatty acids, squalene and diglycerides (Metz et al 1988). The fatty acid triglycerides cannot be used as fuel directly and require slight chemical modification mainly transesterification, pyrolysis and emulsification.…”

Section: Resultsmentioning

confidence: 99%

Isolation and characterization of fatty acid methyl ester (FAME)-producingStreptomycessp. S161 from sheep (Ovis aries) faeces

Wang

Deng

et al. 2013

Lett Appl Microbiol

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Significance and Impact of the Study: Nowadays, production of biodiesel is based on plant oils, animal fats, algal oils and microbial oils. Lipid mostly consists of triacylglycerols (TAG), and conversion of these lipids into fatty acid short-chain alcohol esters (methanol or ethanol) is the final step in biodiesel production. In this study, an oil-producing Streptomyces strain was isolated from sheep faeces. The oil was composed of C 14 -C 16 long-chain fatty acid methyl esters, triglycerides and monoglycerides. This is the first isolated strain-producing biodiesel (FAME) directly from starch. Due to showing cellulase and xylanase activities, the strain would be helpful for converting renewable lignocellulose into biodiesel directly. AbstractAn actinomycete producing oil-like mixtures was isolated and characterized. The strain was isolated from sheep faeces and identified as Streptomyces sp. S161 based on 16S rRNA gene sequence analysis. The strain showed cellulase and xylanase activities. The 1 H nuclear magnetic resonance (NMR) spectra of the mixtures showed that the mixtures were composed of fatty acid methyl esters (52Á5), triglycerides (13Á7) and monoglycerides (9Á1) (mol.%). Based on the gas chromatography-mass spectrometry (GC-MS) analysis, the fatty acid methyl esters were mainly composed of C14-C16 long-chain fatty acids. The results indicated that Streptomyces sp. S161 could produce fatty acid methyl esters (FAME) directly from starch. To our knowledge, this is the first isolated strain that can produce biodiesel (FAME) directly from starch.

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“…To our knowledge only two sources of branched TAG have so far been identified, both of them of bacterial origin. Metz et al [18] used GC-MS to separate and identify intact TAG from Streptomyces avermitilis containing odd-and even-chain FA including iso-and anteiso-FA. Another source of branched TAG is milk; branched FA contained in it are largely derived from bacteria leaving the rumen [14,15].…”

Section: Introductionmentioning

confidence: 99%

RP‐HPLC/MS‐APCI Analysis of Branched Chain TAG Prepared by Precursor‐Directed Biosynthesis with Rhodococcuserythropolis

Schreiberová

Krulikovská

Sigler

et al. 2010

Lipids

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Reversed phase liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (RP-HPLC/MS-APCI) was used to analyze both synthetic triacylglycerols (TAG) having 1-3 branched fatty acids (FA) in the molecule, and natural TAG prepared by precursor directed biosynthesis from valine, leucine and isoleucine and the corresponding branched short-chain acids in cultivations of Rhodococcus erythropolis. The technique made it possible to identify and quantify TAG differing in a single branched-chain FA. Altogether 11 TAG were synthesized, out of which 8 were synthesized stereospecifically. Branched- and straight-chain-TAG were separated and identified while TAG differing only in iso or anteiso FA could not be separated. The APCI mass spectra of iso-, anteiso- and straight-chain TAG were completely identical. The natural material was found to contain 19 TAG having at least one branched FA. Cultivation on six different substrates showed, apart from the presumed and common incorporation of precursors to iso-even, iso-odd and anteiso FA, also some unusual features such as an increase in the content of odd-FA after the addition of Val (attributed to catabolism of Val to propionate) or the appearance of branched monounsaturated FA. The two-sample paired t test, when applied to the TAG, showed that only the pair Val and isobutyrate differ in incorporation into FA--see, e.g. proportions of M/M/O and brM/brM/O (1.2:1.2 and 1.9:1.2, respectively). Also, incorporation of Val (isobutyrate) yielded only TAG having two branched FA in the molecule, whereas Leu and Ile (isovalerate and 2-methyl-butyrate) gave only TAG with a single branched FA in the molecule.

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Characterization of a lipid-rich fraction synthesized by streptomyces avermitilis

Cited by 9 publications

References 16 publications

Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp. NP10

Structural diversity and possible functional roles of free fatty acids of the novel soil isolate Streptomyces sp. NP10

Isolation and characterization of fatty acid methyl ester (FAME)-producingStreptomycessp. S161 from sheep (Ovis aries) faeces

RP‐HPLC/MS‐APCI Analysis of Branched Chain TAG Prepared by Precursor‐Directed Biosynthesis with Rhodococcuserythropolis

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