Characterization of a glycine-rich protein from <i>Rhipicephalus microplus</i>: tissue expression, gene silencing and immune recognition

Leal, Bruna Ferreira; Alzugaray, M. F.; Seixas, Adriana; Vaz, Itabajara da Silva; Ferreira, Carlos Alexandre Sanchez

doi:10.1017/s0031182017001998

Cited by 9 publications

(11 citation statements)

References 94 publications

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“…As a long-term blood feeder, H. dromedarii secrete a cement-like substance to strengthen their attachment to the hosts [37]. These proteins have been used as anti-tick vaccines isolated from other tick species [72–74]. Glycine-rich proteins may play roles other than in the tick-host relationship, especially in embryo development as was proven recently in Rhipicephalus microplus [72].…”

Section: Resultsmentioning

confidence: 99%

Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii

et al. 2019

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Background The hard tick Hyalomma dromedarii is one of the most injurious ectoparasites affecting camels and apparently best adapted to deserts. As long-term blood feeders, ticks are threatened by host defense system compounds that can cause them to be rejected and, ultimately, to die. However, their saliva contains a cocktail of bioactive molecules that enables them to succeed in taking their blood meal. A recent sialotranscriptomic study uncovered the complexity of the salivary composition of the tick H. dromedarii and provided a database for a proteomic analysis. We carried out a proteomic-informed by transcriptomic (PIT) to identify proteins in salivary glands of both genders of this tick species. Results We reported the array of 1111 proteins identified in the salivary glands of H. dromedarii ticks. Only 24% of the proteins were shared by both genders, and concur with the previously described sialotranscriptome complexity. The comparative analysis of the salivary glands of both genders did not reveal any great differences in the number or class of proteins expressed their enzymatic composition or functional classification. Indeed, few proteins in the entire proteome matched those predicted from the transcriptome while others corresponded to other proteins of other tick species. Conclusion This investigation represents the first proteomic study of H. dromedarii salivary glands. Our results shed light on the differences between the composition of H. dromedarii male and female salivary glands, thus enabling us to better understand the gender-specific strategy to feed successfully. Electronic supplementary material The online version of this article (10.1186/s12864-019-6042-1) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii

et al. 2019

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“…Gene knockdown studies suggest some glycine-rich tick saliva proteins may have additional roles, eg. in embryo development (Leal et al, 2018). Besides its potential as an anti-tick and pathogen transmission modulating vaccine, the natural glue and sealant properties of tick cement suggest the potential for developing new medical adhesives (Suppan et al, 2018).…”

Section: Gasket and Holdfastmentioning

confidence: 99%

Wonders of tick saliva

Nuttall

2019

Ticks and Tick-borne Diseases

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The NERC and CEH trademarks and logos ('the Trademarks') are registered trademarks of NERC in the UK and other countries, and may not be used without the prior written consent of the Trademark owner. AbstractSaliva of ticks is arguably the most complex saliva of any animal. This is particularly the case for ixodid species that feed for many days firmly attached to the same skin site of their obliging host. Sequencing and spectrometry technologies combined with bioinformatics are enumerating ingredients in the saliva cocktail. The dynamic and expanding saliva recipe is helping decipher the wonderous activities of tick saliva, revealing how ticks stealthily hide from their hosts while satisfying their gluttony and sharing their individual resources. This review takes a tick perspective on the composition and functions of tick saliva, covering water balance, gasket and holdfast, control of host responses, dynamics, individuality, mate guarding, saliva-assisted transmission, and redundancy. It highlights areas sometimes overlookedfeeding aggregation and sharing of sialomes, and the contribution of salivary gland storage granulesand questions whether the huge diversity of tick saliva molecules is 'redundant' or more a reflection on the enormous adaptability wonderous saliva confers on ticks.

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“…Indeed, these proteins were found to be essential for tick attachment and feeding on the host. Specifically, they were involved in the host’s immune system evasion [56]. Because the high expression of GRPs has been demonstrated in adult female ticks, these proteins have been proposed as potential candidate molecules in the formulation of an anti-tick vaccine cocktail for the biological control of ticks [56].…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, they were involved in the host’s immune system evasion [56]. Because the high expression of GRPs has been demonstrated in adult female ticks, these proteins have been proposed as potential candidate molecules in the formulation of an anti-tick vaccine cocktail for the biological control of ticks [56]. Likewise, a specific protective anti-tick immune response was shown in mice immunised by a recombinant troponin I-like protein from H. longicornis [57].…”

Section: Discussionmentioning

confidence: 99%

Comparative proteomics of the vector Dermacentor reticulatus revealed differentially regulated proteins associated with pathogen transmission in response to laboratory infection with Rickettsia slovaca

et al. 2019

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Background Tick-borne rickettsial diseases are caused by pathogens acquired from hard ticks. In particular, Rickettsia slovaca , a zoonotic infectious bacterium causing tick-borne lymphadenopathy (TIBOLA), is transmitted by the vectors Dermacentor spp. that can be found all over Europe. Although recent studies point out the extreme complexity of bacteria-induced effects in these blood-feeding vectors, the knowledge of individual molecules involved in the preservation and transmission of the pathogen is still limited. System biology tools, including proteomics, may contribute greatly to the understanding of pathogen-tick-host interactions. Methods Herein, we performed a comparative proteomics study of the tick vector Dermacentor reticulatus that was experimentally infected with the endosymbiotic bacterium R. slovaca . Rickettsia -free ticks, collected in the southern region of Slovakia, were infected with the bacterium by a capillary tube-feeding system, and the dynamics of infection was assessed by quantitative PCR method after 5, 10, 15 and 27 days. Results At the stage of controlled proliferation (at 27 dpi), 33 (from 481 profiled) differentially abundant protein spots were detected on a two-dimensional gel. From the aforementioned protein spots, 21 were successfully identified by tandem mass spectrometry. Conclusions Although a few discovered proteins were described as having structural or housekeeping functions, the vast majority of the affected proteins were suggested to be essential for tick attachment and feeding on the host, host immune system evasion and defensive response modulation to ensure successful pathogen transmission. Electronic supplementary material The online version of this article (10.1186/s13071-019-3564-y) contains supplementary material, which is available to authorized users.

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Characterization of a glycine-rich protein from Rhipicephalus microplus: tissue expression, gene silencing and immune recognition

Cited by 9 publications

References 94 publications

Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii

Proteomic informed by transcriptomic for salivary glands components of the camel tick Hyalomma dromedarii

Wonders of tick saliva

Comparative proteomics of the vector Dermacentor reticulatus revealed differentially regulated proteins associated with pathogen transmission in response to laboratory infection with Rickettsia slovaca

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