Characterization of a genome-specific Gypsy-like retrotransposon sequence and development of a molecular marker specific for Dasypyrum villosum (L.)

Zhang, Jie; Long, Hai; Pan, Zhifen; Liang, Jinsong; Yu, Shuiyang; Deng, Guangbing; Yu, Maoqun

doi:10.1007/s12041-013-0218-2

Cited by 9 publications

(3 citation statements)

References 27 publications

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“…Then, 1000 M 1 plants were randomly selected and screened by V-genome-specific marker, DV1 [ 31 ], analyses, and the M 1 plants carrying V-genome-specific bands were used for FISH analyses. In total, 563 M 1 plants were successfully identified using FISH.…”

Section: Resultsmentioning

confidence: 99%

“…Genomic DNA of D. villosum accession PI 491576, common wheat CS, CD-3, 22-12, and 24-20 was extracted from 2-week-old leaves by using the CTAB method [ 67 ]. V-genome-specific SCAR markers of DV1 were synthesized, as described by Zhang et al [ 31 ]. The PLUG primers for the homologous group 3 and the EST primers specific to 3V#4 chromosomes were synthesized using the procedures described by Ishikawa et al [ 43 ] and Li et al [ 37 ], respectively.…”

Section: Methodsmentioning

confidence: 99%

“…Numerous molecular markers have been developed to monitor D. villosum chromatin in the wheat background [ 31 , 32 ], map favorable characteristics on chromosomal regions [ 12 ], and detect resistance genes located on the chromosomes originating from D. villosum [ 33 , 34 ]. However, only a few molecular markers are available for the utilization of D. villosum in wheat breeding.…”

Section: Introductionmentioning

confidence: 99%

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Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust

Zhang

Tang

Lang

et al. 2022

Plants

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The annual species Dasypyrum villosum possesses several potentially valuable genes for the improvement of common wheat. Previously, we identified a new stripe rust-resistant line, the Chinese Spring (CS)–D. villosum 3V#3 (3D) substitution line (named CD-3), and mapped its potential rust resistance gene (designated as YrCD-3) on the 3V#3 chromosome originating from D. villosum. The objective of the present study was to further narrow down the YrCD-3 locus to a physical region and develop wheat-3V#3 introgression lines with strong stripe rust resistance. By treating CD-3 seeds with 60Co γ-irradiation, two CS-3V#3 translocation lines, T3V#3S.3DL and T3DS.3V#3L (termed 22-12 and 24-20, respectively), were identified from the M4 generation through a combination of non-denaturing fluorescence in situ hybridization (ND-FISH) and functional molecular markers. Stripe rust resistance tests showed that the line 22-12 exhibited strong stripe rust resistance similarly to CD-3, whereas 24-20 was susceptible to stripe rust similarly to CS, indicating that YrCD-3 is located on the short arm of 3V#3. The line 22-12 can potentially be used for further wheat improvement. Additionally, to trace 3V#3 in the wheat genetic background, we produced 30 3V#3-specific sequence tag (EST) markers, among which, 11 markers could identify 3V#3S. These markers could be valuable in fine-mapping YrCD-3.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust

Zhang

Tang

Lang

et al. 2022

Plants

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Isolation and application of P genome-specific DNA sequences of Agropyron Gaertn. in Triticeae

Liu

Zhang

et al. 2016

Planta

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Different types of P genome sequences and markers were developed, which could be used to analyze the evolution of P genome in Triticeae and identify precisely wheat- A. cristatum introgression lines. P genome of Agropyron Gaertn. plays an important role in Triticeae and could provide many desirable genes conferring high yield, disease resistance, and stress tolerance for wheat genetic improvement. Therefore, it is significant to develop specific sequences and functional markers of P genome. In this study, 126 sequences were isolated from the degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR) products of microdissected chromosome 6PS. Forty-eight sequences were identified as P genome-specific sequences by dot-blot hybridization and DNA sequences analysis. Among these sequences, 22 displayed the characteristics of retrotransposons, nine and one displayed the characteristics of DNA transposons and tandem repetitive sequence, respectively. Fourteen of 48 sequences were determined to distribute on different regions of P genome chromosomes by fluorescence in situ hybridization, and the distributing regions were as following: all over P genome chromosomes, centromeres, pericentromeric regions, distal regions, and terminal regions. We compared the P genome sequences with other genome sequences of Triticeae and found that the similar sequences of the P genome sequences were widespread in Triticeae, but differentiation occurred to various extents. Additionally, thirty-four molecular markers were developed from the P genome sequences, which could be used for analyzing the evolutionary relationship among 16 genomes of 18 species in Triticeae and identifying P genome chromatin in wheat-A. cristatum introgression lines. These results will not only facilitate the study of structure and evolution of P genome chromosomes, but also provide a rapid detecting tool for effective utilization of desirable genes of P genome in wheat improvement.

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Isolation of two new retrotransposon sequences and development of molecular and cytological markers for Dasypyrum villosum (L.)

et al. 2017

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Dasypyrum villosum is a valuable genetic resource for wheat improvement. With the aim to efficiently monitor the D. villosum chromatin introduced into common wheat, two novel retrotransposon sequences were isolated by RAPD, and were successfully converted to D. villosum-specific SCAR markers. In addition, we constructed a chromosomal karyotype of D. villosum. Our results revealed that different accessions of D. villosum showed slightly different signal patterns, indicating that distribution of repeats did not diverge significantly among D. villosum accessions. The two SCAR markers and FISH karyotype of D. villosum could be used for efficient and precise identification of D. villosum chromatin in wheat breeding.

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Characterization of a genome-specific Gypsy-like retrotransposon sequence and development of a molecular marker specific for Dasypyrum villosum (L.)

Cited by 9 publications

References 27 publications

Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust

Molecular Cytogenetic Identification of the Wheat–Dasypyrum villosum T3DL·3V#3S Translocation Line with Resistance against Stripe Rust

Isolation and application of P genome-specific DNA sequences of Agropyron Gaertn. in Triticeae

Isolation of two new retrotransposon sequences and development of molecular and cytological markers for Dasypyrum villosum (L.)

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