Characterization of a Ferrocene-Containing Polyacrylamide-Based Redox Gel for Biosensor Use

Bu, Hai‐Zhi; Mikkelsen, Susan; English, Ann M.

doi:10.1021/ac00118a007

Cited by 78 publications

(76 citation statements)

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“…This value is similar to other redox polymers [36,37], which suggests that three-dimensional charge propagation in this CS-Fc/MWNTs/GOD composite film is operative with redox polymer, unlike the acrylamide-ferrocene copolymer reported previously in which the redox charge decreased until a stable twodimensional wired enzyme was obtained in the monolayer level [19]. The fast electron-transfer in the CS-Fc/MWNTs film is attributed to the charge hopping between neighboring the conductive MWNTs and Fc redox sites that mediate the effective charge migration through the polymer.…”

Section: Electrochemical Characteristics Of the Modified Electrodessupporting

confidence: 90%

One‐Step Electrochemically Deposited Nanocomposite Film of CS‐Fc/MWNTs/GOD for Glucose Biosensor Application

et al. 2009

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A simple and controllable electrodeposition approach was proposed for one-step construction of glucose biosensors by in situ co-deposition of ferrocene-branched chitosan derivatives (CS-Fc), multiwalled carbon nanotubes (MWNTs), and glucose oxidase (GOD) onto electrode surface. The formation of CS-Fc could not only effectively prevent the leakage of Fc and retain its electrochemical activity, but also provide a biocompatible microenvironment for retaining the native activity of the immobilized biomolecules. Further entrapment of MWNTs into the CS matrix improved electronic conductivity of the biocomposite significantly. The facile procedure of immobilizing GOD and the promising feature of biocomposite will offer a versatile platform to fabricate biosensors and bioelectronic devices.

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Section: Electrochemical Characteristics Of the Modified Electrodessupporting

confidence: 90%

One‐Step Electrochemically Deposited Nanocomposite Film of CS‐Fc/MWNTs/GOD for Glucose Biosensor Application

et al. 2009

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“…A ferrocene is a typical mediator for enzyme biosensors. [1][2][3][4][5][6][7][8][9][10][11][12] Usually, the enzyme and mediators are embedded or immobilized in/onto a cast polymer on an electrode surface. The integrated design for sensor device between enzyme and redox site strongly influences the sensor characteristics.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

Kase

Muguruma

2004

ANAL. SCI.

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We propose an electron transfer-mediated amperometric enzyme biosensor based on plasma-polymerized thin film of dimethylaminomethylferrocene (DMAMF) on a sputtered gold electrode. The DMAMF plasma-polymerized film is deposited directly onto the surface of the electrode under dry conditions. The resulting thin film not only has redox sites but also is extremely thin (∼20 nm), adheres well onto the substrate (electrode), has a flat surface and a highly-crosslinked network structure, and is hydrophilic in nature. Glucose oxidase is densely immobilized onto the surface of DMAMF plasma-polymerized film on the gold electrode. From the electrochemical measurement, the biosensor can cover the wide range of glucose concentration (1.3 -81 mM) at +350 mV of applied potential. The current response of the glucose biosensor was decreased by less than 5% in an aerobic solution as compared to that in an anaerobic solution. These show that the DMAMF plasma-polymerized films play a role as the electron transfer mediators between the reaction center of enzyme and the electrode.

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“…k med between GOD and vinylferrocene was reported to be 3 × 10 7 cm 3 mol -1 s -1 [40], and this value was used unless otherwise stated. D M was taken from the reported value in the redox polymer hydrogels containing ferrocene derivatives as mediators [41,42] , to compare with experimental values [21]. l was estimated to be around 2 nm from the experimentally measured molecular weight of the linear redox polymer, which was synthesised under the same condition as that used for the graft polymerisation onto the carbon black [21].…”

Section: Original Research Papermentioning

confidence: 99%

Modelling of Reaction and Diffusion Processes in a High‐surface‐area Biofuel Cell Electrode Made of Redox Polymer‐grafted Carbon

Tamaki¹,

Ito²,

Yamaguchi³

2009

Fuel Cells

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A mathematical model considering reaction and diffusion processes in biofuel cells, such as enzyme reactions, apparent electron diffusion in the redox polymer, and diffusion of a substrate in a redox polymer film, verified the effectiveness of a high‐surface‐area biofuel cell electrode with a thin, grafted redox polymer layer. The model calculation shows that the rate‐limiting step of apparent electron diffusion can be overcome by using the electrode with a thin redox polymer, even when the redox polymer used has a low apparent electron diffusion coefficient. In the electrode, increase in the second‐order rate constant for the reaction between an enzyme and a mediator above a certain value does not increase the current density, and thus the mediator used in the electrode should have an adequate second‐order rate constant and a more negative redox potential. In addition, increase in the turnover rate of the enzyme and the surface coverage of the enzyme allows an increase in the current density; the surface coverage of the enzyme has the most influence. Based on these calculations, it is important to realise high‐surface‐area electrodes with a thin redox polymer, and with high enzyme loading.

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Characterization of a Ferrocene-Containing Polyacrylamide-Based Redox Gel for Biosensor Use

Cited by 78 publications

References 0 publications

One‐Step Electrochemically Deposited Nanocomposite Film of CS‐Fc/MWNTs/GOD for Glucose Biosensor Application

One‐Step Electrochemically Deposited Nanocomposite Film of CS‐Fc/MWNTs/GOD for Glucose Biosensor Application

Amperometric Glucose Biosensor Based on Mediated Electron Transfer between Immobilized Glucose Oxidase and Plasma-polymerized Thin Film of Dimethylaminomethylferrocene on Sputtered Gold Electrode

Modelling of Reaction and Diffusion Processes in a High‐surface‐area Biofuel Cell Electrode Made of Redox Polymer‐grafted Carbon

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