Characterization of a Facultatively Psychrophilic Bacterium, Vibrio rumoiensis sp. nov., That Exhibits High Catalase Activity

Abstract

A novel facultatively psychrophilic bacterium, strain S-1, which exhibits extraordinarily high catalase activity was isolated from the drain pool of a fish product processing plant that uses H2O2 as a bleaching and microbicidal agent. The catalase activity of the isolate was 1 or 2 orders of magnitude higher than those of Corynebacterium glutamicum,Staphylococcus aureus, Pseudomonas fluorescens, and five other species tested in this study. The strain seemed to possess only one kind of catalase, according to the results of polyacrylamide gel electrophoresis of the cell extract. The optimum temperature for catalase activity was about 30°C, which was about 20°C lower than that for bovine catalase activity. Electron microscopic observation revealed that the surface of the microorganism was covered by blebs. Although the isolate was nonflagellated, its taxonomic position on the basis of physiological and biochemical characteristics and analysis of 16S rRNA sequence and DNA-DNA relatedness data indicated that strain S-1 is a new species belonging to the genus Vibrio. Accordingly, we propose the nameVibrio rumoiensis. The type strain is S-1 (FERM P-14531).

Lipolytic activity of Antarctic cold-adapted marine bacteria (Terra Nova Bay, Ross Sea)

“…As well as Psychrobacter species, Vibrio strains are widely distributed in aquatic environments, especially in association with animals. Although they are generally mesophilic, some cold-adapted Vibrio strains have been isolated (Xu et al 1998;Yumoto et al 1999;Bendt et al 2001). Members of the Pseudoalteromonas genus are recognized as producers of biologically active compounds (Holmström and Kjelleberg 1999;Isnanseytyo and Kamei 2003) and enzymes (Feller et al 1998;Vera et al 1998;Hoyoux et al 2001).…”

A starch‐binding domain identified in α‐amylase (AmyP) represents a new family of carbohydrate‐binding modules that contribute to enzymatic hydrolysis of soluble starch

“…All these Vibrio sp. strains are marine fish (or prawn, crab, rotifer) pathogens except Vibrio rumoiensis [24] and some unreported Vibrio sp. strains cannot identify whether they are marine pathogenic bacteria.…”

Biodiversity among luminescent symbionts from squid of the genera Uroteuthis, Loliolus and Euprymna (Mollusca: Cephalopoda)

“…Our combined analysis produced a phylogeny that was able to resolve Thailand isolates with a relatively high bootstrap value (75%). Within this clade, there is one node (95% jackknife support), containing isolates EHP3, LAR3, EHP5, EHP4 and EHP1, which includes the recently described species Vibrio rumoiensis (Yumoto et al., 1999). This facultative psychrophilic bacterium is known to exhibit high catalase activity, which may explain why Thailand squid symbionts exhibit a closer relationship to identified strains of V. rumoiensis .…”