Characterization of a Dengue Patient Cohort in Recife, Brazil

Abstract

From 2004 to 2006, 658 patients with suspected dengue virus infections were enrolled in a clinical dengue cohort established in Recife, Pernambuco, located at the northeastern region of Brazil. A total of 2,364 blood samples were collected, and serum, plasma, and cells were cryopreserved. Among the suspected cases, 354 (54%) were confirmed as acute DENV-3 infection based on reverse transcription-polymerase chain reaction, virus isolation, and ELISA-IgM. According to WHO criteria, 29.4% of the positive acute cases were classified as dengue fever (DF) and 8.2% of the cases as dengue hemorrhagic fever (DHF), grade 1 or 2. The DHF cases represent 100% of those confirmed in Recife during the period of the study. The dengue cases that did not fulfill the definition of either DHF or DF were classified as DF complicated and accounted for 44.0% of the cases. All the acute cases were classified as either primary or secondary acute dengue virus infections. Secondary infection was predominant in patients with DF; however, there was no predominance of either primary or secondary infections in patients with DHF.

Production of intravenous human dengue immunoglobulin from Brazilian-blood donors

“…This product was found to have high anti-dengue antibody titers of 1:1280 for DENV-2 and 1:160 for DENV-1 and DENV-3. This was expected, since this IVIG was prepared using blood donations from Brazil, which is an endemic area (Cordeiro et al, 2007). In Brazil, three serotypes are currently in circulation, DENV-1, 2, and 3, while infection by DENV-4 is more restricted to the North Region (Roraima, Manaus and Belém) (Brazil, 2010(Brazil, , 2011a(Brazil, , 2011b.…”

Aumento da carga de dengue no Brasil e unidades federadas, 2000 e 2015: análise do Global Burden of Disease Study 2015

“…The risk of death, in this group, is associated to the difficulty in handling the disease in a population with high frequency of comorbidities 30 . In addition to that, in areas where the occurrence of dengue is relatively new, such as in Minas Gerais and in most part of Brazil 31 , older people are not entirely immune and therefore, they have an increased risk of secondary infection 32 and, consequently, greater risk of a more severe disease. In Brazil, the co-circulation of viral serotypes of dengue is frequent, with alternated predominant serotypes.…”

Prospective birth cohort in a hyperendemic dengue area in Northeast Brazil: methods and preliminary results

“…Saúde Pública, Rio de Janeiro, 32(1):e00095815, jan, 2016 Table 2 Comparison of maternal and child characteristics at baseline stratified by children followed and those not followed in the birth cohort study. 2,17 . This maternal anti-dengue immune profile seems to be different from studies conducted in pregnant women in Asian countries where the majority of pregnant women were immune against two or more DENV serotypes 5,8,9 .…”

“…Dengue cases tracked in febrile infants were laboratory-confirmed by reverse-transcriptase polymerase chain reaction (RT-PCR) and IgM-ELISA. RT-PCR was performed according to a standardized protocol described elsewhere 17 . Viral RNA was extracted from serum samples using QIAquick PCR purification kits (Qiagen, Valencia, USA).…”