Characterization of a Collection of Brassica carinata Genotypes for in vitro Culture Response and Mode of Shoot Regeneration

Gil-Humanes, Javier; Martı́n, A.; Barro, Francisco

doi:10.4236/ajps.2011.21003

Cited by 5 publications

(5 citation statements)

References 30 publications

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“…12 Similar to canola, B. carinata may also have a potential application in the animal industry. However, as far as we know, great efforts and progress have been made in breeding works for the development of B. carinata in various applications, 4,13,14 but nutrient availability and quality evaluation of carinata seed for animals are extremely lacking.…”

Section: ■ Introductionmentioning

confidence: 99%

Studies on Brassica carinata Seed. 1. Protein Molecular Structure in Relation to Protein Nutritive Values and Metabolic Characteristics

Xin

Falk

2013

J. Agric. Food Chem.

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The objectives of this study were to investigate (1) the protein chemical profile, (2) the protein subfractions partitioned by the Cornell Net Carbohydrate and Protein System (CNCPS), (3) the rumen crude protein (CP) degradation kinetics, (4) the protein supply predicted by the DVE/OEB system, (5) the protein structural features using a Fourier transform infrared (FTIR) spectroscopic technique with attenuated total reflectance (ATR), and (6) the correlations between protein intrinsic structural features and nutritional profiles in three strains of Brassica carinata in yellow and brown seed coats, with comparison to canola seed as a reference. The results showed that carinata seed strains were different in both nutritional values and IR absorbance within the protein spectral region (ca. 1720-1482 cm(-1)). The comparison between yellow and brown B. carinata seeds indicated that the former was lower in acid detergent insoluble crude protein (ADICP; P = 0.002) and undegradable protein fraction (PC; P = 0.002) and greater in the degradable (D) fraction (P = 0.004) and true absorbed protein in the small intestine (DVE; P = 0.02) as well as feed milk value (FMV; P = 0.02) than the latter. The brown canola seed (Brassica napus L.) was also not in full accordance with B. carinata seed on these parameters. The FTIR studies showed significant differences in protein amide II peak height, amide I peak area, and β-sheet height among different B. carinata strains. However, multivariate spectral analyses indicated a similarity in protein structural makeup in these four kinds of oilseed. The not very strong correlations shown in this study implied that the limited sample size and narrow range in biological and spectral variation might be responses for the weak relationships between chemical profile and mid-IR spectral data. Further studies using sufficient samples with wide and diverse range in nutritional properties are needed to illustrate the actual relationship between spectroscopic data and nutritional profiles in oilseeds.

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Section: ■ Introductionmentioning

confidence: 99%

Studies on Brassica carinata Seed. 1. Protein Molecular Structure in Relation to Protein Nutritive Values and Metabolic Characteristics

Xin

Falk

2013

J. Agric. Food Chem.

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“…Although many years of research have been conducted on the breeding and agronomics of B. carniata , ,, we still know little about its nutritive value and biodegradation behavior, which may allow for its use in animal diets. As documented in our accompanying part 1 of this series of study, characterization of protein structure features in relation to protein supply and availability obtained from yellow and brown carinata seeds has been carried out.…”

Section: Introductionmentioning

confidence: 99%

Studies on Brassica carinata Seed. 2. Carbohydrate Molecular Structure in Relation to Carbohydrate Chemical Profile, Energy Values, and Biodegradation Characteristics

Xin

Falk

2013

J. Agric. Food Chem.

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The objectives of this study were to investigate (1) the carbohydrate chemical profile, (2) the energy values, (3) the rumen neutral detergent fiber (NDF) degradation kinetics, (4) the carbohydrate-related functional group structural features using a Fourier transform infrared (FTIR) spectroscopic technique with attenuated total reflectance (ATR), and (5) the correlations between carbohydrate intrinsic structural features and nutritional profiles in three strains of Brassica carinata in yellow and brown seed coats, with comparison to canola seed as a reference. The results showed that yellow B. carinata strains 111000EM and AAC A100 were lower for contents of neutral detergent fiber (NDF), acid detergent fiber (ADF), acid detergent lignin (ADL), and carbohydrate (CHO) and higher for contents of total digestible nutrients (TDN), energy values, and effective degradable NDF (EDNDF) than brown-seeded 110915EM. In comparison, brown canola seed (Brassica napus L.) had more fiber content and less EDNDF. Also, carinata strains showed significantly different IR intensities in structural carbohydrate (SCHO), cellulosic compounds (CELC), and total CHO profiles. These structural variations might be one of the possible reasons for various fiber profile and biodegradation characteristics for ruminants in oilseeds. However, multivariate analyses within carbohydrate regions indicated there were still some structural relationships among the four oilseed samples. Moreover, the correlation study showed that the changes of CELC and CHO peak intensities were highly related with some changes in CHO chemical profile, energy values, and in situ NDF degradation kinetics in B. carinata and canola seeds. Further study with a large sample size is still necessary to figure out whether CHO molecular spectral information could be used to predict nutrient values and biological behavior in oilseeds.

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“…QTL associated with the shoot regeneration efficiency in Arabidopsis have also been identified (Lall et al 2004), and the genetic regulation of gene expression during shoot development is attracting considerable attention. A large variation in the regeneration frequency of 51 genotypes of B. carinata was observed (Gil-Humanes et al 2011). In B. napus, there was a huge variation ranging from 0% to 91% in the 100 cultivars tested (Ono et al 1994).…”

Section: Genotypementioning

confidence: 93%

Advances in in vitro culture of the Brassicaceae crop plants

Park¹,

Ahmed

Kim

et al. 2012

Journal of Plant Biotechnology

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Characterization of a Collection of Brassica carinata Genotypes for in vitro Culture Response and Mode of Shoot Regeneration

Abstract: ABSTRACT

Cited by 5 publications

References 30 publications

Studies on Brassica carinata Seed. 1. Protein Molecular Structure in Relation to Protein Nutritive Values and Metabolic Characteristics

Studies on Brassica carinata Seed. 1. Protein Molecular Structure in Relation to Protein Nutritive Values and Metabolic Characteristics

Studies on Brassica carinata Seed. 2. Carbohydrate Molecular Structure in Relation to Carbohydrate Chemical Profile, Energy Values, and Biodegradation Characteristics

Advances in in vitro culture of the Brassicaceae crop plants

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