Characterization of a Cell-Assembled extracellular Matrix and the effect of the devitalization process

Magnan, Laure; Labrunie, Gaëlle; Marais, Sébastien; Rey, Sylvie; Dusserre, Nathalie; Bonneu, Marc; Lacomme, Sabrina; Gontier, Étienne; L’Heureux, Nicolas

doi:10.1016/j.actbio.2018.10.006

Cited by 21 publications

(28 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It could be seen that the expression of decorin decreased with increasing GelMa concentrations, which was similar to the case of MMP-9. Decorin is a component of the cellular matrix and is commonly found binding to type I collagen fibrils [50]. From the above observation, it was hypothesized that increasing concentrations of gelatin led to increased secretion of MMP-2, which then led to increased remodeling, seen in the form of lower levels of decorin in our results.…”

Section: Protein Expression Of Hdf-laden Scaffoldsupporting

confidence: 54%

Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior

Shie

Lee

et al. 2020

Polymers

View full text Add to dashboard Cite

Gelatin-methacryloyl (GelMa) is a very versatile biomaterial widely used in various biomedical applications. The addition of methacryloyl makes it possible to have hydrogels with varying mechanical properties due to its photocuring characteristics. In addition, gelatin is obtained and derived from natural material; thus, it retains various cell-friendly motifs, such as arginine-glycine-aspartic acid, which then provides implanted cells with a friendly environment for proliferation and differentiation. In this study, we fabricated human dermal fibroblast cell (hDF)-laden photocurable GelMa hydrogels with varying physical properties (5%, 10%, and 15%) and assessed them for cellular responses and behavior, including cell spreading, proliferation, and the degree of extracellular matrix remodeling. Under similar photocuring conditions, lower concentrations of GelMa hydrogels had lower mechanical properties than higher concentrations. Furthermore, other properties, such as swelling and degradation, were compared in this study. In addition, our findings revealed that there were increased remodeling and proliferation markers in the 5% GelMa group, which had lower mechanical properties. However, it was important to note that cellular viabilities were not affected by the stiffness of the hydrogels. With this result in mind, we attempted to fabricate 5–15% GelMa scaffolds (20 × 20 × 3 mm3) to assess their feasibility for use in skin regeneration applications. The results showed that both 10% and 15% GelMa scaffolds could be fabricated easily at room temperature by adjusting several parameters, such as printing speed and extrusion pressure. However, since the sol-gel temperature of 5% GelMa was noted to be lower than its counterparts, 5% GelMa scaffolds had to be printed at low temperatures. In conclusion, GelMa once again was shown to be an ideal biomaterial for various tissue engineering applications due to its versatile mechanical and biological properties. This study showed the feasibility of GelMa in skin tissue engineering and its potential as an alternative for skin transplants.

show abstract

Section: Protein Expression Of Hdf-laden Scaffoldsupporting

confidence: 54%

Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior

Shie

Lee

et al. 2020

Polymers

View full text Add to dashboard Cite

show abstract

“…A zoom performed on the ECM part allowed to detect the characteristic banding pattern of collagens fibrils (Fig1D). Of note, fibril-forming collagens types I, III, and V, characterized by their ability to assemble into highly orientated supramolecular fibrils, are the main components of the human dermal extracellular matrix (Gelse 2003) and of the human dermal substitutes as assessed by proteomics (Magnan et al 2018). This result correlated with the second-harmonic generation (SHG) signal, detected by two-photon microscopy, which confirms the proper organization of these fibrillar collagens within our tissue model (Fig1E).…”

Section: Resultssupporting

confidence: 78%

“…These calibrated electric parameters were applied to a sophisticated in vitro tissue-engineered human dermal substitute, produced through the self-assembly approach in which cells produce their own extracellular matrix (Athanasiou et al 2013). Thus, the final tissue substitutes are rich in endogenous ECM proteins, as demonstrated by proteomic analyses (Magnan et al 2018), demonstrating its complex composition and similarity to the native dermis.…”

Section: Introductionmentioning

confidence: 99%

Pulsed Electric Fields Induce Extracellular Matrix Remodeling through Matrix Metalloproteinases Activation and Decreased Collagen Production

Gouarderes

Ober

Doumard

et al. 2022

Journal of Investigative Dermatology

View full text Add to dashboard Cite

“…Recently, a thorough characterization of cell-assembled ECM and the effect of the devitalization process by dehydration was performed. This study revealed the complex composition of more than 50 different proteins and no changes in the biological architecture and mechanical properties after devitalization [ 93 ]. Despite these positive results [ 94 , 95 ], the extremely long production time remained an issue for future clinical use.…”

Section: Strategies To Generate or Mimic Endothelial Basement Membranesmentioning

confidence: 99%

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Lau

Gossen

Lendlein

2021

IJMS

View full text Add to dashboard Cite

Insufficient endothelialization of cardiovascular grafts is a major hurdle in vascular surgery and regenerative medicine, bearing a risk for early graft thrombosis. Neither of the numerous strategies pursued to solve these problems were conclusive. Endothelialization is regulated by the endothelial basement membrane (EBM), a highly specialized part of the vascular extracellular matrix. Thus, a detailed understanding of the structure–function interrelations of the EBM components is fundamental for designing biomimetic materials aiming to mimic EBM functions. In this review, a detailed description of the structure and functions of the EBM are provided, including the luminal and abluminal interactions with adjacent cell types, such as vascular smooth muscle cells. Moreover, in vivo as well as in vitro strategies to build or renew EBM are summarized and critically discussed. The spectrum of methods includes vessel decellularization and implant biofunctionalization strategies as well as tissue engineering-based approaches and bioprinting. Finally, the limitations of these methods are highlighted, and future directions are suggested to help improve future design strategies for EBM-inspired materials in the cardiovascular field.

show abstract

Characterization of a Cell-Assembled extracellular Matrix and the effect of the devitalization process

Abstract: Characterization of a Cell-Assembled extracellular Matrix (CAM) and effect of the devitalization process.

Cited by 21 publications

References 50 publications

Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior

Effects of Gelatin Methacrylate Bio-ink Concentration on Mechano-Physical Properties and Human Dermal Fibroblast Behavior

Pulsed Electric Fields Induce Extracellular Matrix Remodeling through Matrix Metalloproteinases Activation and Decreased Collagen Production

Designing Cardiovascular Implants Taking in View the Endothelial Basement Membrane

Contact Info

Product

Resources

About