Characterization of a canine long-term T cell line (DLC 01) established from a dog with Sézary syndrome and producing retroviral particles

Abstract

The canine DLC 01 cell line derives from a lymph node of a dog with Sé zary syndrome. The DLC 01 cell phenotype is CD4 ؊ , CD8؉ , CD45 ؉ , DQ ؉ , similar to that of original cells after treatment with dimethylsulfoxide or phorbol myristate. Canine cutaneous T cell lymphoma are usually CD4Ϫ , CD8 ؉ in contrast to their human counterparts which are CD4 ؉ , CD8 ؊ . Therefore, the DLC 01 cell line appears to be a unique model to study the mechanism of all surface molecule expression in vitro. Viral particles with retrovirus type-C morphology were found in ultrathin sections of DLC 01 cell pellets. Retroviral particles are spontaneously produced after the 50th cell passage or after induction with 0.5% dimethylsulfoxide. This is the first description of a dog lymphoid cell line spontaneously growing and producing a retrovirus. It was found to share several features in common with feline and murine leukemia viruses.

Epitheliotrophic Cutaneous T Cell Lymphoma (CETL) in a Labrador Retriever Dog. A Case Report

“…Sezary syndrome is characterized by dermatological clinical signs accompanied with lymphocytic and neutrophilic leukocytosis (59.000/mm 3 ) (Ghernati et al, 1999). This case showed several dermatological signs that were further confirmed by histopathology, including fungoid mycoses and a progressive increase of leukocyte counts until neutrophilia with left deviation, anemia and hypoproteinemia, but normal platelet count.…”

Canine Large Granular Lymphocyte Leukemia and its Derived Cell Line Produce Infectious Retroviral Particles

“…Interestingly, this cell line, termed DLC 01, also produces a type C retrovirus associated with a manganesedependent RT activity. 3 This retrovirus has now been fully characterized (in preparation) and belongs to the mammalian type C oncovirus genus. Preliminary results indicate that the viruses isolated from the DLC 01 and DLC 02 cell lines are related but distinct.…”

Síndrome de Sézary em cadela

“…Em cães e seres humanos, a síndrome de Sézary é uma forma de linfoma epiteliotrópico de ocorrência rara, evolução subaguda e fatal (Thrall et al, 1984;Ghernati et al,1999). Na avaliação imunoistoquímica, observa-se a expressão de CD3 (linfoma de células T) (Fournel-Fleury et al, 2002), e na citometria de fluxo a expansão clonal em sangue periférico de CD4 e CLA (antígenos de linfócitos cutâneos) (Wojdylo et al, 2005).…”