Characterization of a bacterioruberin‐producing Haloarchaea isolated from the marshlands of the Odiel river in the southwest of Spain

Abstract

In this work, we describe the isolation, identification, pigment characterization, and optimization of the culture conditions for a haloarchaea strain isolated from salt evaporation ponds in the Odiel river, at Southwest of Spain. The haloarchaea belongs to the genus Halorobrum, as deduced from the analysis of its 16S rRNA encoding gene and has been designated as Halorubrum sp. SH1. The growth conditions for the new strain were optimized studying temperature, NaCl concentration, agitation rate and light intensity. The C50-carotenoids, bacterioruberin, and its derivatives bisanhydrobacterioruberin and trisanhydrobacterioruberin, were found to be the predominant pigments produced by this strain of Halorubrum, as determined using HPLC-DAD and UHPLC-ESI-MS/MS techniques. This extremely halophilic archaeon could be a good candidate for the production of bacterioruberins of high added-value due to their coloring, antioxidant, and possible anticancer properties. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 32:592-600, 2016.

Haloterrigena sp. Strain SGH1, a Bacterioruberin-Rich, Perchlorate-Tolerant Halophilic Archaeon Isolated From Halite Microbial Communities, Atacama Desert, Chile

“…The identification and composition of C-50 carotenoids in strain Haloterrigena sp. SGH1 was based on the data analyses of their chromatographic migrations, UV-Vis spectra, spectral fine structure and MS fragmentation patterns (Fong et al, 2001;Mandelli et al, 2012;De la Vega et al, 2016). The absorbance spectrum of the methanol extract of strain SGH1 showed the photometric fingerprint or three-fingered maxima profile that include maxima at 468, 494, and 526 nm (Figure 3), as it has been previously reported in archaea (Mandelli et al, 2012; Results are shown as mean ± standard error with n values in parentheses.…”

“…Lorantfy et al, 2014; De la Vega et al, 2016). This was a preliminary evidence for the presence of BR-type molecules in SGH1 and the presence of a BR-type molecule in strain SGH1 cells was confirmed by Raman spectroscopy (Table 4) which showed three major Raman displacement signals (1.502, 1.147, and 995 cm −1 ) that were in close proximity with those informed for extracts from other haloarchaea (Jehlička et al, 2014;Dina et al, 2017).…”

“…Absorption spectrum (350-600 nm) of carotenoid extracts were obtained in a Shimadzu spectrophotometer UV-1601 and absorbance values at 494 nm were used to compute total SGH1 carotenoids concentration in SGH1 methanol extracts, using the following equation and the extinction coefficient for BR in methanol (2,660 g% −1 cm −1 ) (Mandelli et al, 2012;De la Vega et al, 2016):…”

“…C-50 carotenoid identification from strain Haloterrigena sp. SGH1 was conducted by data analyses of Raman spectroscopy, chromatographic migrations, UV-Vis spectra, spectral fine structure and MS fragmentation patterns (Fong et al, 2001;Mandelli et al, 2012;Jehlička et al, 2014;De la Vega et al, 2016;Dina et al, 2017).…”

Halorubrum sp. TBZ112, an Extremely Halophilic Carotenoid- Producing Archaeon Isolated from Urmia Lake

“…11 With the exception of our previous study reported recently, 6 to the best of our knowledge, there are two articles that describe carotenoid production from a Halorubrum strain. 12,13 Urmia Lake is the largest brine lake in the Middle East and the second-largest saltwater lake in the earth. 14 Even so, very few studies of the lake have been reported, and no study was published on the carotenoidproducing prokaryotics that live in this hyper-saline lake.…”