Characterization of a bacterial collar and rhizome rot of banana (Musa paradisiaca) caused by strains of Erwinia chrysanthemi pv. paradisiaca

Nagrale, Dipak T.; Borkar, S. G.; Gawande, S. P.; Mandal, Asit; Raut, S.A.

doi:10.31018/jans.v5i2.349

Cited by 5 publications

(6 citation statements)

References 15 publications

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“…An inhibition zone was observed around the filter paper disc containing streptomycin solution whereas no inhibition zone was observed around the filter paper disc containing erythromycin. P. carotovorum is reported to be resistant to erythromycin and susceptible to streptomycin (Dickey and Victoria, 1980;Snehalatharani and Khan, 2010;Nagrale et al, 2013;Akbar et al, 2015). Since the isolates in this study are resistant to erythromycin and susceptibility to streptomycin, it is confirmed that they belong to Pectobacterium carotovorum.…”

Section: Kbsupporting

confidence: 71%

“…Deteriorating of the pseudostem has started and finally the plant chop down as the pseudostem got entirely rotten and emitted foul smell, after 16 days of inoculation. Nagaraj et al, (2012) and Nagrale et al, (2013) reported that complete rotting of healthy rhizome bits were observed after 37 DAI under in vivo condition. The similar plant symptoms were observed by Thomas et al, (2008) who demonstrated that plants inoculated with pathogenic Pectobacterium carotovorum subsp.…”

Section: Pathogenicity and Virulence Testmentioning

confidence: 99%

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Molecular Detection of the Causative Agent of Soft Rot (Pectobacterium carotovorum subsp carotovorum) in Banana (Musa sp.)

Ragavi¹,

Muthamilan²,

Nakkeeran³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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Soft rot disease of Banana caused by Pectobacterium carotovorum subsp. Carotovorum is found to be a serious disease and cause considerable yield loss. Common cultivars viz., Grand Naine, Rasthali and Nendran are extremely prone to this disease. Understanding the disease diagnosis and pathogen virulence is important in management programs. The pathogen was isolated from infected rhizomes from five locations of Theni district of Tamil Nadu. Pathogenicity of all isolates was confirmed by in vitro inoculation in rhizome bits and in vivo inoculation in pseudostems of three months old tissue cultured plants of cv. Grand Naine. The virulent isolates Icb 4 and Ikg 2 were characterized according to the morphological and microscopic characteristics, along with the physiological and biochemical assays; which indicates that the bacteria are gram negative, rod shaped, peritrichously flagellated. The colonies form characteristic deep cavities or pits in Crystal violet pectate medium (CVP). Results of biochemical tests showed that isolates Icb 4 and Ikg 2 have a positive reaction for catalase, H 2 S production and nitrate reductase and a negative reaction for oxidase test, Indole test and gelatin liquefaction. The results determining the optimal temperature for the growth of isolates Icb 4, and Ikg 2 have revealed that the temperature at 30 ºC -37 ºC were suitable to obtain profuse growth of Pectobacterium. Total DNA extracted from the isolates Icb 4, and Ikg 2 were subjected to PCR analysis of 16S-23S intergenic transcribed spacer region. PCR amplified 1.5 kb amplicons were sequenced. Sequence Homology and phylogeny analyses had shown that the isolates Icb 4 and Ikg 2 were belonged to Pectobacterium carotovorum subsp. carotovorum.

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Section: Kbsupporting

confidence: 71%

Section: Pathogenicity and Virulence Testmentioning

confidence: 99%

Molecular Detection of the Causative Agent of Soft Rot (Pectobacterium carotovorum subsp carotovorum) in Banana (Musa sp.)

Ragavi¹,

Muthamilan²,

Nakkeeran³

et al. 2019

Int.J.Curr.Microbiol.App.Sci

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“…The coloration occurs as a result of reductive deamination of tryptophan by the action of tryptophanases (Schaad et al, 2001), which confirms that these isolates belong to the species D. chrysanthemi. These results agree with those of Nagrale et al (2013) for E. chrysanthemi pv. paradisiaca, Ashmawy et al (2015), Bhupendra and Yogendra (2015), and Vasundhara and Thammaiah (2017), who observed that D. chrysanthemi caused production of indole acid when performing this biochemical test to characterize bacterial isolates.…”

Section: A B Csupporting

confidence: 91%

“…Colonies of D. chrysanthemi, D. paradisiaca and P. carotovorum subsp. carotovorum were isolated from different crops, including bananas using NA culture medium, with similar results to those obtained in the present work (Nagrale et al, 2013;Akbar et al, 2015;Czajkowski et al, 2015;Vasundhara and Thammaiah, 2017).…”

Section: A B Csupporting

confidence: 90%

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CHARACTERIZATION OF RHIZOME AND PSEUDOSTEM WET ROT OF ORGANIC BANANA (Musa sp.) IN PIURA, PERU

Rafael-Rutte¹,

Zavala²,

Maldonado³

et al. 2022

Chil. j. agric. anim. sci.

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Organic banana (Musa sp.) is one of the most important export crops in Peru, especially in the Department of Piura. The cultivation of the crop is mainly affected by biotic factors, resulting in important economic losses. The aim of this research was to characterize the causal agent of banana bacteriosis disease in Piura. Bacterial isolates were extracted from rhizome and pseudostem samples of organic bananas with typical symptoms of soft rot from different banana growing areas of Piura. Sixty colonies were obtained, while 10 isolates were selected for presenting cultural and morphological characteristics similar to those of Pectobacterium (01MB, 04MB, 05MB, 06MB and 10MB) and Dickeya (02MB, 03MB, 08MB, 07MB and 09MB); different biochemical and molecular tests were conducted. The pathogenicity of the isolates was confirmed by in vitro and in vivo inoculation of rhizome pieces and pseudostems from cultivated plants. The 10 isolates were gram negative and with perimeter flagella. The biochemical tests showed that all the isolates were positive on nutrient agar medium, Mac Conkey, catalase, gelatin hydrolysis and growth at 37°C, whereas they were negative on King's B medium, nitrate reductase and oxidase tests. Isolates 02MB, 03MB, 08MB, 07MB and 09MB also tested positive for erythromycin and indole, while isolates 01MB, 04MB, 05MB, 06MB and 10MB were positive for CVP (Crystal violet pectate). Molecular identification and phylogenetic analysis confirmed that isolates 01MB, 04MB, 05MB and 10MB corresponded to Pectobacterium carotovorum subsp. carotovorum; isolates 03MB and 08MB corresponded to Dickeya chrysanthemi; and 02MB and 07MB corresponded to Dickeya paradisiaca and Klebsiella variicola, respectively. It was concluded that rhizome and pseudostem wet rot of banana grown organically in Piura is caused by the bacterial complex formed by P. carotovorum subsp. carotovorum, D. chrysanthemi, D. paradisiaca and K. variicola. This is valuable information for the implementation of appropriate disease management practices in the area.

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RNAi-mediated protection against banana diseases and pests

Ghag

Ganapathi

2019

3 Biotech

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Pests and pathogens restrict the production potential of many crop plants. The losses incurred due to pests and diseases are huge threatening food security. Management strategies include use of chemical pesticides which can be detrimental to human health and environment and other physical and biological methods which have serious limitations. An alternative would be to utilize the advanced technology such as RNA interference (RNAi) to engineer disease resistance in crop plants. The phenomenon of RNAi is very well studied in organisms across genera and found to be conserved. Taking advantage of this, dsRNAs have been delivered into pests and pathogens and showed significant growth inhibition. Banana is susceptible to various groups of pathogens which results in poor yield. The proof-of-principle studies using RNAi technology have already been demonstrated in banana to develop resistance to two important groups of pathogens. Transgenic banana plants expressing small interfering RNA targeting BBTV and Fusarium pathogen have shown high level of resistance. In this review, we summarize and discuss the studies utilizing RNAi as a strategy to develop resistance to major banana diseases and encourage further research in exploiting RNAi-based resistance in other crop plants.

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Characterization of a bacterial collar and rhizome rot of banana (Musa paradisiaca) caused by strains of Erwinia chrysanthemi pv. paradisiaca

Cited by 5 publications

References 15 publications

Molecular Detection of the Causative Agent of Soft Rot (Pectobacterium carotovorum subsp carotovorum) in Banana (Musa sp.)

Molecular Detection of the Causative Agent of Soft Rot (Pectobacterium carotovorum subsp carotovorum) in Banana (Musa sp.)

CHARACTERIZATION OF RHIZOME AND PSEUDOSTEM WET ROT OF ORGANIC BANANA (Musa sp.) IN PIURA, PERU

RNAi-mediated protection against banana diseases and pests

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