RNAi-mediated protection against banana diseases and pests

Ghag, Siddhesh B.; Ganapathi, T. R.

doi:10.1007/s13205-019-1650-7

Cited by 7 publications

(5 citation statements)

References 57 publications

(50 reference statements)

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“…VIGS is widely used to study plant gene functions under abiotic stress (such as drought [26], salt stress [27], chilling stress [28]) and during development [29]. In addition, RNAi has become an effective tool for studying the function of plant genes under the biotic stresses caused by various pests, e.g., whiteflies [30], and for crop protection from pathogens, such as insects, viruses, and fungi [31][32][33][34][35]. RNAi, in addition to model plants, is also used in fruit trees [36,37].…”

Section: Virus-induced Gene Silencing (Vigs)mentioning

confidence: 99%

Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

Motorina,

Galimova,

Battulina

et al. 2024

IJMS

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At present, there are a variety of different approaches to the targeted regulation of gene expression. However, most approaches are devoted to the activation of gene transcription, and the methods for gene silencing are much fewer in number. In this review, we describe the main systems used for the targeted suppression of gene expression (including RNA interference (RNAi), chimeric transcription factors, chimeric zinc finger proteins, transcription activator-like effectors (TALEs)-based repressors, optogenetic tools, and CRISPR/Cas-based repressors) and their application in eukaryotes—plants and animals. We consider the advantages and disadvantages of each approach, compare their effectiveness, and discuss the peculiarities of their usage in plant and animal organisms. This review will be useful for researchers in the field of gene transcription suppression and will allow them to choose the optimal method for suppressing the expression of the gene of interest depending on the research object.

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Section: Virus-induced Gene Silencing (Vigs)mentioning

confidence: 99%

Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

Motorina,

Galimova,

Battulina

et al. 2024

IJMS

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“…As an alternative to the fungicides commonly used to control the disease, the external application of RNAi is a promising alternative in controlling fungal diseases in the field and during postharvest handling because it does not require transgenic modification, uses specific sequences, and allows the action on highly specific targets (Islam & Sherif, 2020; Ghag & Ganapathi, 2019; Wang & Jin, 2017; Wang et al., 2016).…”

Section: Biofungicides Based On Rnai and Gene Silencing To Control Postharvest Fruit Pathogensmentioning

confidence: 99%

Control of postharvest fungal diseases in fruits using external application of RNAi

Filho

Silva

Cipriano

et al. 2021

Journal of Food Science

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Contamination with a variety of filamentous fungi can cause deterioration of food and agricultural products. Fungal contaminations reduce the quality and the shelf life of fresh fruits and are one of the main causes of economic loss in the global fresh fruit industry. Although chemical fungicides are effective and traditionally used to control postharvest fungal diseases, they are harmful to human health. In this context, use of RNA interference (RNAi)-based fungicides is a promising alternative strategy. Spray-induced gene silencing (SIGS) is an innovative RNAi-based approach for silencing target genes in phytopathogens. This review aims to discuss the recent findings on the use of RNAi-based fungicides to control the postharvest spoilage of fresh fruits. Practical Application: Control of postharvest fungal diseases is one of the most important strategies to make food available to consumers longer. In this sense, the external application of RNAi seems to be technologically advantageous and efficient as it helps to maintain the characteristics of plant products. In this sense, this review discussed what is possible to find in the literature regarding this new technology.

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“…There are good examples of RNAi-mediated silencing of targeted genes as in wheat against F. culmorum (Chen et al 2016) and F. graminearum (Koch et al 2016;Kamran et al 2020), banana against F. oxysporum f. sp. cubense (Ghag and Ganapathi 2019), tomato against F. oxysporum f. sp. lycopersici (Singh et al 2020;Tetorya and Rajam 2018), barley against F. graminearum (Koch et al 2016) and tobacco against F. verticillioides (Tinoco et al 2010).…”

Section: Host-induced Gene Silencingmentioning

confidence: 99%

Potato dry rot disease: current status, pathogenomics and management

et al. 2020

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Potato dry rot disease caused by Fusarium species is a major threat to global potato production. The soil and seed-borne diseases influence the crop stand by inhibiting the development of potato sprouts and cause severe rots in seed tubers, table and processing purpose potatoes in cold stores. The symptoms of the dry rot include sunken and wrinkled brown to black tissue patches on tubers having less dry matter and shriveled flesh. Fungal infection accompanied by toxin development in the rotten tubers raises more concern for consumer health. The widespread dry rot causing fungal species (Fusarium graminearum) is reported to have a hemibiotrophic lifestyle. A cascade of enzymes, toxins and small secreted proteins are involved in the pathogenesis of these hemibiotrophs. With the availability of the genome sequence of the most devastating species Fusarium sambucinum, it is important to identify the potential pathogenicity factors and small secreted proteins that will help in designing management strategies. Limited resistant cultivars and the emergence of fungicide-resistant strains have made it more threatening for potato cultivation and trade. Several novel fungicide molecules (Azoxystrobin, chlorothalonil and fludioxonil), are found very effective as tuber treatment chemicals. Besides, many beneficial bioagents and safer chemicals have shown antibiosis and mycoparasitism against this pathogen. Germplasm screening for dry rot resistance is important to assist the resistance breeding program for the development of resistant cultivars. This review aims to draw attention to the symptomatology, infection process, pathogenomics, the role of toxins and management approaches for potato dry rot disease, which is very much critical in designing better management strategies.

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RNAi-mediated protection against banana diseases and pests

Cited by 7 publications

References 57 publications

Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

Systems for Targeted Silencing of Gene Expression and Their Application in Plants and Animals

Control of postharvest fungal diseases in fruits using external application of RNAi

Potato dry rot disease: current status, pathogenomics and management

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