Characterization of 7-Methylguanosine Identified Biochemical Recurrence and Tumor Immune Microenvironment in Prostate Cancer

Abstract

Prostate cancer (PCa) has a high incidence rate, mortality rate, and biochemical recurrence (BCR) rate. 7-Methylguanosine (m7G), as one of the RNA modifications, has been considered to be actively involved in cancer-related translation disorders in recent years. Therefore, we first used The Cancer Genome Atlas (TCGA) database to identify prognosis and m7G-related long non-coding RNAs (lncRNAs). Then we randomly divided the samples into the training set and test set and then constructed and verified the m7G lnRNA prognostic model (m7Gscore) by the least absolute shrinkage and selection operator (LASSO) regression analysis. The m7Gscore has been proved to be an independent marker of BCR-free survival in patients with PCa. Furthermore, the m7Gscore was significantly correlated with the tumor immune microenvironment (TIME) and somatic mutation of PCa patients and had the potential to be an indicator for the selection of drug treatment. We also clustered TCGA cohort into three m7G-related patterns (C1, C2, and C3). The Kaplan–Meier survival analysis revealed that C1 had the best BCR-free survival and C3 had the worst. The TIME was also significantly distinct among the three m7G-related patterns. According to the TIME characteristics of the patterns, we defined C1, C2, and C3 as immune-desert phenotype, immune-inflamed phenotype, and immune-excluded phenotype, respectively.

M7G-related molecular subtypes can predict the prognosis and correlate with immunotherapy and chemotherapy responses in bladder cancer patients

“…They also identified that the m7G-related signature could predict the prognosis of prostate cancer. Similarly, Liu et al [ 20 ] also identified the prognostic value of m7G-related lncRNAs in prostate cancer. Moreover, three studies demonstrated the prognostic value of m7G-related mRNAs, lncRNAs, and miRNAs in clear cell renal cell carcinoma [ 16 – 18 ].…”

“…Furthermore, in the field of urological tumors, three studies demonstrated the prognostic value of m7G-related mRNAs, lncRNAs and miRNAs in clear cell renal cell carcinoma [ 16 – 18 ]. Meanwhile, M7G-related genes could influence the immune infiltration of prostate cancer [ 19 , 20 ]. However, a few studies have discussed the correlation between m7G and BC through single m7G-related gene and review [ 13 , 21 ].…”

m6A/ m1A /m5C/m7G-related methylation modification patterns and immune characterization in prostate cancer

“…In recent years, m 7 G has been thought to be actively implicated in cancer-related translation problems. The m 7 G-score has been shown to be an independent measure of BCR-free survival in patients with PCa ( Xin et al, 2022 ). Additionally, recent research has shown that RNA modification regulators may serve as biomarkers for cancer diagnosis and prognosis surveillance ( Haruehanroengra et al, 2020 ).…”

Epigenetic Modifications in Prostate Cancer Metastasis and Microenvironment

“…[ 72 ]. At present, it is believed that m 7 G has a potential role in regulating the tumor immune microenvironment and metastasis of prostate cancer [ 73 ].…”