Characterization of 5-HT receptor subtypes mediating contraction in human umbilical vein. 2. Evidence of involvement of 5-HT 1B receptors using functional studies

Rogines-Velo, María Pía; Pelorosso, Facundo Germán; Zold, Camila Lidia; Brodsky, Paula Tamara; Rothlin, Rodolfo Pedro

doi:10.1007/s00210-002-0637-8

Cited by 12 publications

(5 citation statements)

References 15 publications

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“…In those cases where the slope of the Schild's plot was not significantly different from unity, the regression was recalculated with Schild's slope constrained to unity ( Neubig et al ., 2003 ) and the affinity value obtained was then referred as p K B . In the case of pirenzepine (0.17 μ M ) vs ACh, pirenzepine (0.17 μ M ) vs McN‐A‐343 and pFHHSiD (0.03 μ M ) vs ACh, antagonist affinities were obtained by ‘single concentration’ analysis (assuming a Schild's regression slope of 1) according to the equation: p A 2 =−log [Antagonist]+log( r −1) ( Lachnit et al ., 1997 ; Rogines‐Velo et al ., 2002a ). Statistical analysis was performed by means of paired or unpaired Student's t ‐test and one‐way analysis of variance followed by Tukey's post‐test, when appropiate.…”

Section: Methodsmentioning

confidence: 99%

Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein

Pujol-Lereis

Hita

Gobbi

et al. 2006

British J Pharmacology

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1 The present study attempted to pharmacologically characterize the muscarinic receptor subtypes mediating contraction of human umbilical vein (HUV). 2 HUV rings were mounted in organ baths and concentration-response curves were constructed for acetylcholine (ACh) (pEC 50 : 6.1670.04; maximum response 80.0071.98% of the responses induced by serotonin 10 mM). The absence of endothelium did not modify the contractile responses of ACh in this tissue. 3 The role of cholinesterases was evaluated: neither neostigmine (acetylcholinesterase inhibitor) nor iso-OMPA (butyrylcholinesterase inhibitor) modified ACh responses. When both enzymes were simultaneously inhibited, a significantly but little potentiation was observed (control: pEC 50 6.3370.03; double inhibition: pEC 50 6.5770.05). 4 Atropine, nonselective muscarinic receptors antagonist, inhibited ACh-induced contraction (pK B 9.67). The muscarinic receptors antagonists pirenzepine (M 1 ), methoctramine (M 2 ) and pFHHSiD (M 3 ) also antagonized responses to ACh. The affinity values estimated for these antagonists against responses evoked by ACh were 7.58, 6.78 and 7.94, respectively. On the other hand, PD 102807 (M 4 selective muscarinic receptors antagonist) was ineffective against ACh-induced contraction. 5 In presence of a blocking concentration of pirenzepine, pFHHSiFD produced an additional antagonism activity on ACh-induced responses. 6 The M 1 muscarinic receptors agonist McN-A-343 produced similar maximum but less potent responses than ACh in HUV. The calculated pA 2 for pirenzepine against McN-A-343 induced responses was 8.54. 7 In conclusion, the data obtained in this study demonstrate the role of M 1 muscarinic receptor subtypes and suggest the involvement of M 3 muscarinic receptor subtypes in ACh-induced vasoconstriction in HUV rings. In addition, the vasomotor activity evoked by ACh does not seem to be modulated by endothelial factors, and their enzymatic degradation appears to have little functional relevance in this tissue.

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Section: Methodsmentioning

confidence: 99%

Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein

Pujol-Lereis

Hita

Gobbi

et al. 2006

British J Pharmacology

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“…Serotonin is usually considered to be a neurotransmitter, but surprisingly, only 1% of serotonin in the human body is found in the CNS [ 4 ]. The remaining 99% is found in other tissues, primarily plasma, the gastro-intestinal tract, and immune tissues, where serotonin acts as a hormone regulating various physiological functions including vasodilation[ 5 ], clotting[ 6 ], recruitment of immune cells [ 7 - 9 ], gastro-intestinal motility,[ 10 ] and initiation of uterine contraction [ 11 , 12 ]. Serotonin also has peripheral functions in a wide variety of animal phyla [ 13 - 16 ] and is similar in chemical structure to auxin, which regulates plant cell shape, growth, and movement [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

An overlooked connection: serotonergic mediation of estrogen-related physiology and pathology

et al. 2005

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BackgroundIn humans, serotonin has typically been investigated as a neurotransmitter. However, serotonin also functions as a hormone across animal phyla, including those lacking an organized central nervous system. This hormonal action allows serotonin to have physiological consequences in systems outside the central nervous system. Fluctuations in estrogen levels over the lifespan and during ovarian cycles cause predictable changes in serotonin systems in female mammals.DiscussionWe hypothesize that some of the physiological effects attributed to estrogen may be a consequence of estrogen-related changes in serotonin efficacy and receptor distribution. Here, we integrate data from endocrinology, molecular biology, neuroscience, and epidemiology to propose that serotonin may mediate the effects of estrogen. In the central nervous system, estrogen influences pain transmission, headache, dizziness, nausea, and depression, all of which are known to be a consequence of serotonergic signaling. Outside of the central nervous system, estrogen produces changes in bone density, vascular function, and immune cell self-recognition and activation that are consistent with serotonin's effects. For breast cancer risk, our hypothesis predicts heretofore unexplained observations of the opposing effects of obesity pre- and post-menopause and the increase following treatment with hormone replacement therapy using medroxyprogesterone.SummarySerotonergic mediation of estrogen has important clinical implications and warrants further evaluation.

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“…Contraction of HUV by serotonin is mediated by both 5-HT 2A and 5-HT 1B receptors [29][30][31]. Cord blood serotonin levels were reported to be 2.8 times higher after VD compared to PCS in bovine [32].…”

Section: Discussionmentioning

confidence: 99%

Advantages of human umbilical vein scaffolds derived from cesarean section vs. vaginal delivery for vascular tissue engineering

Hoenicka¹,

Jacobs²,

Huber³

et al. 2008

Biomaterials

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Umbilical vein function and birth mode Abstra ctThe current study investigated whether the mode of delivery and the mode of sample collection affect the functional properties of umbilical veins as scaffolds for vascular tissue engineering purposes. HUV from planned c-sections (PCS) showed a 1.7-fold higher maximum contraction with potassium chloride compared to spontaneous vaginal deliveries (VD, p=0.029). The maximum contractions with histamine were 2.0-fold and 2.9-fold higher in the PCS and emergency c-section (ECS) groups, respectively, compared to the VD group (p=0.003). The dose-response curves of serotonin were shifted to the right approx. 6-fold and 5-fold in the VD group compared to PCS and ECS, respectively (p=0.009). There were no differences between the birth groups in terms of tetrazolium dye reduction, platelet adhesion, and the structural integrity. The release of the antithrombotic compound prostacyclin from vessels of the PCS and ECS groups was 6.6-fold and 3.5-fold higher, respectively, than in the VD group (p<0.001). There was no correlation between the duration of ischemia and any of the functional parameters. This study provides evidence that vessels obtained from PCS are to be preferred for tissue engineering purposes, as they can be harvested in a sterile fashion and show superior vasoconstrictor responses and antithrombotic properties. The data also support a once-per-day pickup schedule for umbilical cords without a deterioration of the functional properties.

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Characterization of 5-HT receptor subtypes mediating contraction in human umbilical vein. 2. Evidence of involvement of 5-HT 1B receptors using functional studies

Cited by 12 publications

References 15 publications

Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein

Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein

An overlooked connection: serotonergic mediation of estrogen-related physiology and pathology

Advantages of human umbilical vein scaffolds derived from cesarean section vs. vaginal delivery for vascular tissue engineering

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