Advantages of human umbilical vein scaffolds derived from cesarean section vs. vaginal delivery for vascular tissue engineering

Abstract

Umbilical vein function and birth mode Abstra ctThe current study investigated whether the mode of delivery and the mode of sample collection affect the functional properties of umbilical veins as scaffolds for vascular tissue engineering purposes. HUV from planned c-sections (PCS) showed a 1.7-fold higher maximum contraction with potassium chloride compared to spontaneous vaginal deliveries (VD, p=0.029). The maximum contractions with histamine were 2.0-fold and 2.9-fold higher in the PCS and emergency c-section (ECS) groups, respectively, compared to the VD group (p=0.003). The dose-response curves of serotonin were shifted to the right approx. 6-fold and 5-fold in the VD group compared to PCS and ECS, respectively (p=0.009). There were no differences between the birth groups in terms of tetrazolium dye reduction, platelet adhesion, and the structural integrity. The release of the antithrombotic compound prostacyclin from vessels of the PCS and ECS groups was 6.6-fold and 3.5-fold higher, respectively, than in the VD group (p<0.001). There was no correlation between the duration of ischemia and any of the functional parameters. This study provides evidence that vessels obtained from PCS are to be preferred for tissue engineering purposes, as they can be harvested in a sterile fashion and show superior vasoconstrictor responses and antithrombotic properties. The data also support a once-per-day pickup schedule for umbilical cords without a deterioration of the functional properties.

Tissue Engineering of Small Caliber Vessel Grafts from Human Umbilical Veins

“…HUV were found to contract to sero tonin and to U46619, to possess a biphasic stress-strain rela tionship [6], to retain part of the contractile function and of tissue viability after cryopreservation, and to release the an tithrombotic agent prostacyclin even after denuding. Vascu lar function and antithrombotic properties were best in HUV from cesarean sections [7].…”

Neue Verwendungsmöglichkeiten von Nachgeburtsgewebe für die Regenerative Medizin

“…Die Besiedelbarkeit dieses Materials mit vaskulären Zellen wurde gezeigt. Auch die Umbilikalarterie wurde auf ihre Eignung für das vaskuläre Tissue Engineering untersucht [ 35 , 36 ] Um nach Möglichkeit die optimale Auswahl bei den Nabelschnü-ren für Tissue-Engineering-Anwendungen zu treff en, wurde untersucht, ob der Geburtsmodus einen Einfl uss auf die Umbilikalvenenfunktion hat [ 39 ] . Die Nabelschnüre wurden hierbei in 3 Gruppen unterteilt: (1) vaginale Geburten sowie (2) Literatur…”

Evaluation of decellularized human umbilical vein (HUV) for vascular tissue engineering - comparison with endothelium-denuded HUV

“…We have previously investigated human umbilical veins (HUVs) as a source for TEBV and demonstrated that endothelium‐denuded HUVs can be seeded with autologous cells from CABG patients to form a confluent and flow‐resistant neoendothelium (Hoenicka et al ., , , ). The present study was undertaken to investigate the utility of decellularized HUV as scaffolds for vascular tissue engineering and to compare these two approaches.…”