Characterization of 5-HT receptor subtypes mediating contraction in human umbilical vein. 1. Evidence of involvement of 5-HT 2A receptors using functional and radioligand binding assays

Rogines-Velo, María Pía; Pelorosso, Facundo Germán; Zold, Camila Lidia; Nowak, W; Pesce, Guido; Sardi, S. Pablo; Brodsky, Paula Tamara; Rothlin, Rodolfo Pedro

doi:10.1007/s00210-002-0636-9

Cited by 11 publications

(8 citation statements)

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“…Thus, when a functional study is performed using ring preparations, the circular layer is the one evaluated, whereas the longitudinal layer is tested using longitudinal strips segments and both layers using helical strips segments. In previous reports, significant differences in potency or efficacy of several vasoactive agents were observed between rings and strips preparation of HUV ( Rogines‐Velo et al ., 2002b ; Daray et al ., 2003 ). Hence, the anatomical differences described regarding the tissue preparations employed provides an explanation for the pharmacological differences observed between the results published by Altura et al .…”

Section: Discussionmentioning

confidence: 77%

Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein

Pujol-Lereis

Hita

Gobbi

et al. 2006

British J Pharmacology

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1 The present study attempted to pharmacologically characterize the muscarinic receptor subtypes mediating contraction of human umbilical vein (HUV). 2 HUV rings were mounted in organ baths and concentration-response curves were constructed for acetylcholine (ACh) (pEC 50 : 6.1670.04; maximum response 80.0071.98% of the responses induced by serotonin 10 mM). The absence of endothelium did not modify the contractile responses of ACh in this tissue. 3 The role of cholinesterases was evaluated: neither neostigmine (acetylcholinesterase inhibitor) nor iso-OMPA (butyrylcholinesterase inhibitor) modified ACh responses. When both enzymes were simultaneously inhibited, a significantly but little potentiation was observed (control: pEC 50 6.3370.03; double inhibition: pEC 50 6.5770.05). 4 Atropine, nonselective muscarinic receptors antagonist, inhibited ACh-induced contraction (pK B 9.67). The muscarinic receptors antagonists pirenzepine (M 1 ), methoctramine (M 2 ) and pFHHSiD (M 3 ) also antagonized responses to ACh. The affinity values estimated for these antagonists against responses evoked by ACh were 7.58, 6.78 and 7.94, respectively. On the other hand, PD 102807 (M 4 selective muscarinic receptors antagonist) was ineffective against ACh-induced contraction. 5 In presence of a blocking concentration of pirenzepine, pFHHSiFD produced an additional antagonism activity on ACh-induced responses. 6 The M 1 muscarinic receptors agonist McN-A-343 produced similar maximum but less potent responses than ACh in HUV. The calculated pA 2 for pirenzepine against McN-A-343 induced responses was 8.54. 7 In conclusion, the data obtained in this study demonstrate the role of M 1 muscarinic receptor subtypes and suggest the involvement of M 3 muscarinic receptor subtypes in ACh-induced vasoconstriction in HUV rings. In addition, the vasomotor activity evoked by ACh does not seem to be modulated by endothelial factors, and their enzymatic degradation appears to have little functional relevance in this tissue.

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Section: Discussionmentioning

confidence: 77%

Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein

Pujol-Lereis

Hita

Gobbi

et al. 2006

British J Pharmacology

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“…(1972) found significant differences in potency and efficacy of several vasoactive agents between helical and longitudinal HUV strip preparations. Recently, significative pharmacological differences were observed among our group employing rings and the group of Tuncer employing helical strips in the characterization of 5HT 2A receptor in HUV (Rogines‐Velo et al ., 2002b). Taking into account the pharmacological differences observed between both layers of HUV and the relevance of the circular layer in the in vivo vasoconstriction, reducing blood flow from the placenta to the foetus in different pathophysiological conditions, we consider important the pharmacological studies employing ring preparations.…”

Section: Discussionmentioning

confidence: 98%

Pharmacological characterization of prostanoid receptors mediating vasoconstriction in human umbilical vein

Daray

Minvielle

Puppo

et al. 2003

British J Pharmacology

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1 This study was undertaken to characterize pharmacologically the prostanoid receptor subtypes mediating contraction in human umbilical vein (HUV). 2 HUV rings were mounted in organ baths and concentration -response curves to U-46619 (TXA 2 mimetic) were constructed in the absence or presence of SQ-29548 or ICI-192,605 605 (100 nm) was completely ineffective against PGF 2a or fluprostenol. In addition, lack of antagonistic effect of AH 6809 (1 mm) against PGF 2a was observed. 5 In conclusion, the findings obtained with TP-selective agonist and antagonists provide strong evidence of the involvement of TP receptors promoting vasoconstriction in HUV. Furthermore, the action of the natural and synthetic EP receptor agonists appears to be mediated via TP receptors. On the other hand, the results employing FP receptor agonists and antagonists of different prostanoid receptors suggest the presence of FP receptors mediating vasoconstriction in this vessel.

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“…Contraction of HUV by serotonin is mediated by both 5-HT 2A and 5-HT 1B receptors [29][30][31]. Cord blood serotonin levels were reported to be 2.8 times higher after VD compared to PCS in bovine [32].…”

Section: Discussionmentioning

confidence: 99%

Advantages of human umbilical vein scaffolds derived from cesarean section vs. vaginal delivery for vascular tissue engineering

Hoenicka¹,

Jacobs²,

Huber³

et al. 2008

Biomaterials

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Umbilical vein function and birth mode Abstra ctThe current study investigated whether the mode of delivery and the mode of sample collection affect the functional properties of umbilical veins as scaffolds for vascular tissue engineering purposes. HUV from planned c-sections (PCS) showed a 1.7-fold higher maximum contraction with potassium chloride compared to spontaneous vaginal deliveries (VD, p=0.029). The maximum contractions with histamine were 2.0-fold and 2.9-fold higher in the PCS and emergency c-section (ECS) groups, respectively, compared to the VD group (p=0.003). The dose-response curves of serotonin were shifted to the right approx. 6-fold and 5-fold in the VD group compared to PCS and ECS, respectively (p=0.009). There were no differences between the birth groups in terms of tetrazolium dye reduction, platelet adhesion, and the structural integrity. The release of the antithrombotic compound prostacyclin from vessels of the PCS and ECS groups was 6.6-fold and 3.5-fold higher, respectively, than in the VD group (p<0.001). There was no correlation between the duration of ischemia and any of the functional parameters. This study provides evidence that vessels obtained from PCS are to be preferred for tissue engineering purposes, as they can be harvested in a sterile fashion and show superior vasoconstrictor responses and antithrombotic properties. The data also support a once-per-day pickup schedule for umbilical cords without a deterioration of the functional properties.

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Characterization of 5-HT receptor subtypes mediating contraction in human umbilical vein. 1. Evidence of involvement of 5-HT 2A receptors using functional and radioligand binding assays

Cited by 11 publications

References 0 publications

Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein

Pharmacological characterization of muscarinic receptor subtypes mediating vasoconstriction of human umbilical vein

Pharmacological characterization of prostanoid receptors mediating vasoconstriction in human umbilical vein

Advantages of human umbilical vein scaffolds derived from cesarean section vs. vaginal delivery for vascular tissue engineering

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