Characterization of 3′untranslated region (3′UTR) of the MICB gene

Pan, Fenghua; Liu, XueXiang; Huang, Jufang; Tian, Wei

doi:10.1016/j.humimm.2013.01.028

Cited by 6 publications

(22 citation statements)

References 33 publications

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“…11725 11735 11745 11755 11765 11775 11785 11795 11805 | 11995 12005 12015 12025 12035 12045 12055 12065 12075 | | | | | | | | | MICB-UTR1 TCCTGCCAAC AGAAGGTTTG CTGAGGGCAT TCACTCCATG GTGCTCATTG GAGTTATCTA CTGGGTCATC TAGAGCCTAT TGTTTGAGGA MICB-UTR2 - 12085 12095 12105 12115 12125 12135 12145 12155 12265 12275 12285 12295 12305 12315 12325 12335 12345 The purpose of this study was to investigate polymorphism of the 3'UTR of MICA and MICB genes in European IHW cell line DNA, as previous studies have focussed on a specific population of Chinese Han. 13,17 We previously investigated the 5'UTR of MICA and MICB using the same reference DNA samples 9 and this study extends those findings and provides a more complete picture of the relationship between 5'UTR promoter, coding and 3'UTR polymorphisms and implications for ligand expression levels and functional potential of the various MICA/B alleles. We identified 17 MICA extended haplotypes when considering the allele type and 3'UTR polymorphism.…”

Section: Micb-utr1supporting

confidence: 59%

“…Including the two novel sequence types, we identified six of the nine MICB 3'UTR types among IHW cell line DNA. Of those we did not observe, the MICA‐null haplotype (MICB‐UTR3) had an allele frequency of 2.9% and MICB‐UTR6, and − 7 were rare (<1%) in Chinese Han individuals . As observed with MICA 3'UTR polymorphism, the majority of MICB‐UTR typed IHW cell lines had either MICB‐UTR1 or UTR2, with UTR1 being the most common.…”

Section: Resultsmentioning

confidence: 77%

“…A MICA promoter fragment of 647 bp and MICB fragment of 581 bp was amplified by polymerase chain reaction (PCR) and sequenced in both directions for analysis of positions −560 to −1. This polymorphic region in the 5'UTR of both genes was shown to contain most regulatory elements involved in promoter transcriptional activity as discussed in our previous report and studies published by Luo et al and Pan et al…”

Section: Methodsmentioning

confidence: 99%

“…Within the MICB-3'UTR, there are seven known polymorphic positions derived from five nucleotide substitutions and two insertion/deletion variable positions. 13 Together, these polymorphisms make up seven distinct sequences or MICB 3'UTR types, designated MICA-UTR1-7. In the present study, we further characterised this polymorphism to include one more nucleotide substitution and an extended deletion.…”

Section: Micb 3'utr Polymorphism and Extended Haplotypes Of The Micmentioning

confidence: 99%

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Diversity and characterisation of polymorphic 3' untranslated region haplotypes of MICA and MICB genes

Cox

Hernandez

Danby

et al. 2018

HLA

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MICA and MICB genes encode ligands that interact with the natural killer (NK) cell activating receptor, NKG2D. These ligands display a highly polymorphic allelic repertoire, although the true functional significance of this polymorphism remains elusive. We previously reported additional polymorphism in the 5' untranslated region (UTR) proximal promoter region of these genes by sequencing international histocompatibility workshop (IHW) cell line DNA promoter and coding regions. The present study extends this analysis by further characterising the 3'UTR region of the same IHW reference panel to achieve a more complete understanding of MICA and MICB haplotype diversity and possible functional relevance. We found 17 extended MICA haplotypes encompassing the coding region and 3'UTR, including four novel haplotypes identified in IHW cell line DNA. This increased to 21 when also considering the 5'UTR proximal promoter region. Analysis of the MICB 3'UTR revealed two novel sequences in cell lines KLO and WIN designated MICB‐UTR8 and UTR9, respectively. A total of 11 MICB haplotypes were identified in this study and five were unique. The present study, characterising MICA/B 3'UTR polymorphism utilising IHW reference cell lines, could be useful for future studies investigating the role of microRNA in post‐transcriptional repression of gene expression and for immunotherapy strategies to combat cancer progression.

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Section: Micb-utr1supporting

confidence: 59%

Section: Resultsmentioning

confidence: 77%

Section: Methodsmentioning

confidence: 99%

Section: Micb 3'utr Polymorphism and Extended Haplotypes Of The Micmentioning

confidence: 99%

See 2 more Smart Citations

Diversity and characterisation of polymorphic 3' untranslated region haplotypes of MICA and MICB genes

Cox

Hernandez

Danby

et al. 2018

HLA

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“…However, information on sequence variation in promoter region of different MICB alleles from more diverse ethnic groups is still limited. Recently, we studied the coding region (exons 2–5) and the 3′ UTR of MICB gene in a Chinese Han population recruited from central Inner Mongolia Autonomous Region, northern China . Here we extended our study to investigate the variation of the 5′ promoter of MICB locus in this population.…”

Section: Introductionmentioning

confidence: 99%

The 5′ promoter region of MHC class I chain‐related gene B

Pan

Luo

et al. 2014

Tissue Antigens

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In this study, the 5' promoter region of MHC class I chain-related gene B (MICB) was investigated in 104 healthy, unrelated Han individuals recruited from northern China, using polymerase chain reaction (PCR)-sequencing methodology. Fifteen variable sites were detected, which showed a mosaic pattern of significant global linkage disequilibrium (LD). Eleven different 5' promoter haplotypes were identified. MICB 5' promoter haplotype-9 carrying CT deletion at positions -139/-138, which is associated with decreased MICB promoter activity, was present in 26.9% of MICB alleles and was linked to MICB*002:01, MICB*008, and MICB*014 in this population. In addition, rs3828913 (position -176) and rs3828914 (position -152) were located adjacent to HSRE and GC box in MICB promoter, respectively. Sixteen extended haplotypes (EH) incorporating the MICB 5' promoter and MICB coding region were observed in this population, eight of which were in significant LD. Phylogenetic analysis of 5' promoter refined MICB sub-lineage structure indicated that MICB*005:02, the most common MICB allele, consists of five sub-lineages. Ewens-Watterson homozygosity statistics at MICB 5' promoter region were consistent with neutral expectations. Our study has shed new insight into MICB genetic variation at population level, suggesting that binding sites for transcription factors in MICB promoter could be interrupted by polymorphisms within this region, resulting in allele-specific regulation. The data will facilitate the understanding of regulation of MICB gene transcription, and will inform studies of evolution of the major histocompatibility complex (MHC) gene complex.

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