Characterization of 13 polymorphic microsatellite loci for two land snail species, <i>Trochulus villosus</i> and <i>T. sericeus</i> (Gastropoda: Pulmonata: Hygromiidae)

Dépraz, A.; Rathey, E.; Hausser, Jacques

doi:10.1111/j.1471-8286.2007.02055.x

Cited by 5 publications

(3 citation statements)

References 9 publications

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“…Furthermore, six microsatellite loci were amplified: TROA3, TROA4, TROA5, TROA6, TROB5 and TROB105. All microsatellite loci, their length and repeat size along with amplification conditions were previously described by Dépraz et al [44]. Amplification was performed in three multiplex reactions (two loci in each multiplex) with a Qiagen Multiplex PCR Kit (in 10 μl volume) according to the producer’s manual.…”

Section: Methodsmentioning

confidence: 99%

Ongoing Speciation and Gene Flow between Taxonomically Challenging Trochulus Species Complex (Gastropoda: Hygromiidae)

et al. 2017

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Geographical isolation, selection and genetic drift can cause the geographical diversification of populations and lead to speciation. Land snail species in the genus Trochulus show overlaps in geographical ranges as well as in morphology, but genetic data do not always support the species-level taxonomy based on morphological characters. Such a group offers an excellent opportunity to explore the processes involved. We have addressed the problem by determining the status of the restricted endemic T. graminicola within the larger context of Trochulus taxonomy. We used an integrated approach based on morphological features, ecological preferences and two molecular markers: mitochondrial COI sequences and microsatellites. Comparison of these results demonstrated: (i) conchological distinction of T. striolatus and T. sericeus; (ii) anatomical, ecological and genetic differentiation of T. graminicola and (iii) concordance between morphological characters and mtDNA markers in T. striolatus. Moreover, our data showed an intricate evolutionary history within the genus Trochulus, which can be best explained by: (i) recent or ongoing gene flow between taxa or (ii) their large ancestral polymorphism. Both of these hypotheses suggest that diversification within this group of snails has occurred relatively recently. The mismatches between species defined on morphology and on molecular genetics indicate the complexity of the processes involved in the diversification of this genus.

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Section: Methodsmentioning

confidence: 99%

Ongoing Speciation and Gene Flow between Taxonomically Challenging Trochulus Species Complex (Gastropoda: Hygromiidae)

et al. 2017

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“…The 172 individuals were scored at eight microsatellite loci: TROA3, TROA4, TROA5, TROA105, TROA122, TROB6, TROB108 and TROB111. PCR conditions were according to Dépraz et al . (in press).…”

Section: Methodsmentioning

confidence: 99%

Postglacial recolonization at a snail's pace (Trochulus villosus): confronting competing refugia hypotheses using model selection

et al. 2008

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The localization of Last Glacial Maximum (LGM) refugia is crucial information to understand a species' history and predict its reaction to future climate changes. However, many phylogeographical studies often lack sampling designs intensive enough to precisely localize these refugia. The hairy land snail Trochulus villosus has a small range centred on Switzerland, which could be intensively covered by sampling 455 individuals from 52 populations. Based on mitochondrial DNA sequences (COI and 16S), we identified two divergent lineages with distinct geographical distributions. Bayesian skyline plots suggested that both lineages expanded at the end of the LGM. To find where the origin populations were located, we applied the principles of ancestral character reconstruction and identified a candidate refugium for each mtDNA lineage: the French Jura and Central Switzerland, both ice-free during the LGM. Additional refugia, however, could not be excluded, as suggested by the microsatellite analysis of a population subset. Modelling the LGM niche of T. villosus, we showed that suitable climatic conditions were expected in the inferred refugia, but potentially also in the nunataks of the alpine ice shield. In a model selection approach, we compared several alternative recolonization scenarios by estimating the Akaike information criterion for their respective maximum-likelihood migration rates. The 'two refugia' scenario received by far the best support given the distribution of genetic diversity in T. villosus populations. Provided that fine-scale sampling designs and various analytical approaches are combined, it is possible to refine our necessary understanding of species responses to environmental changes.

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“…The individuals belonging to the two mitochondrial lineages in focus were scored at 6 microsatellite loci – TRO A6, TRO A111, TRO B5, TRO B108, TRO B111 and TRO B112 – following the published PCR conditions [ 53 ]. Amplifications were run on an ABI 3100 capillary sequencer (Applied Biosystems) and scored with G ENEMAPPER v.3 (Applied Biosystems).…”

Section: Methodsmentioning

confidence: 99%

A species delimitation approach in the Trochulus sericeus/hispiduscomplex reveals two cryptic species within a sharp contact zone

2009

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Background: Mitochondrial DNA sequencing increasingly results in the recognition of genetically divergent, but morphologically cryptic lineages. Species delimitation approaches that rely on multiple lines of evidence in areas of co-occurrence are particularly powerful to infer their specific status. We investigated the species boundaries of two cryptic lineages of the land snail genus Trochulus in a contact zone, using mitochondrial and nuclear DNA marker as well as shell morphometrics.

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Characterization of 13 polymorphic microsatellite loci for two land snail species, Trochulus villosus and T. sericeus (Gastropoda: Pulmonata: Hygromiidae)

Cited by 5 publications

References 9 publications

Ongoing Speciation and Gene Flow between Taxonomically Challenging Trochulus Species Complex (Gastropoda: Hygromiidae)

Ongoing Speciation and Gene Flow between Taxonomically Challenging Trochulus Species Complex (Gastropoda: Hygromiidae)

Postglacial recolonization at a snail's pace (Trochulus villosus): confronting competing refugia hypotheses using model selection

A species delimitation approach in the Trochulus sericeus/hispiduscomplex reveals two cryptic species within a sharp contact zone

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