A species delimitation approach in the Trochulus sericeus/hispiduscomplex reveals two cryptic species within a sharp contact zone

Dépraz, A.; Hausser, Jacques; Pfenninger, Markus

doi:10.1186/1471-2148-9-171

Cited by 53 publications

(46 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The non-monophyly of Partula taeniata on both COI and 28S trees is a good example: this result may indicate that species delineations are in this case not accurately assessed. Once again, FTA cards are particularly useful from a conservation perspective: the lack of knowledge on the status of the populations (endangered or not), often severely impacted by habitat destruction and introduction of invasive species and in which the taxonomic delimitation of species are often unclear (Depraz et al 2009;Uit de Weerd 2008), would push the collector to apply the precautionary principle and to not destroy the specimens. In the particular case of species delimitation and description, the FTA card method could be also very interesting.…”

Section: Discussionmentioning

confidence: 99%

Foot mucus stored on FTA® cards is a reliable and non-invasive source of DNA for genetics studies in molluscs

Régnier

Gargominy

Falkner

et al. 2010

Conservation Genet Resour

View full text Add to dashboard Cite

In this study, a non-invasive method for DNA sampling of terrestrial molluscs using mucus stored on FTA cards is described. DNA was isolated from FTA cards impregnated with terrestrial mollusc mucus, which were collected from four mollusc families. DNA was stored for approximately 2 months at room temperature. For two mollusc families, DNA was extracted from foot tissue as a cross-check against the DNA obtained from mucus. Amplification of mitochondrial and nuclear DNA was successful for all mucus samples and in all cases the sequences matched those obtained from tissue samples, but also sequences from the same species, genus or family (depending on their availability) from GenBank. These results show that isolating mucus on FTA cards is a very efficient alternative to sampling tissue for genetic studies of terrestrial molluscs, and particularly for conservation genetic studies. This non-invasive method is rapid, reliable and very easy to put in practice in field situations.

show abstract

Section: Discussionmentioning

confidence: 99%

Foot mucus stored on FTA® cards is a reliable and non-invasive source of DNA for genetics studies in molluscs

Régnier

Gargominy

Falkner

et al. 2010

Conservation Genet Resour

View full text Add to dashboard Cite

show abstract

“…This genus has frequently been the focus of taxonomic questions, which have been addressed using morphological (Focart, 1965; Gittenberger, Backhuys & Ripken, 1970; Schileyko, 1978; Falkner, 1995; Falkner, Ripken & Falkner, 2002; Proćków, 2009; Duda et al ., 2011) and genetic data (Pfenninger et al ., 2005; Dépraz, Hausser & Pfenninger, 2009; Kruckenhauser et al ., 2014). The species with the widest distribution within the genus is T. hispidus (Linnaeus, 1758).…”

Section: Introductionmentioning

confidence: 99%

“…Proćków, 2009). Based on molecular analyses, some authors have suggested splitting T. hispidus into several cryptic species (Pfenninger et al ., 2005; Dépraz et al ., 2009). In a survey of Trochulus species from Germany, Switzerland and France, Pfenninger et al (2005) found several highly distinct mt clades which could, however, not be classified unambiguously.…”

Section: Introductionmentioning

confidence: 99%

“…In a survey of Trochulus species from Germany, Switzerland and France, Pfenninger et al (2005) found several highly distinct mt clades which could, however, not be classified unambiguously. Due to the complicated taxonomic situation and the ambiguous differentiation of T. hispidus and T. sericeus , Dépraz et al (2009) suggested that these taxa should be subsumed under the term ‘ T. hispidus/sericeus complex’. We have subsumed such snails appearing in the various mt clades detected by Kruckenhauser et al .…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differentiation in the Trochulus hispidus complex and related taxa (Pulmonata: Hygromiidae): morphology, ecology and their relation to phylogeography

Duda

Kruckenhauser

Sattmann

et al. 2014

Journal of Molluscan Studies

View full text Add to dashboard Cite

In this study we investigated the morphology and ecology of representatives of the taxonomically ambiguous genus Trochulus. The main focus was on the T. hispidus complex, which comprises several genetically highly divergent mitochondrial clades, as determined in a parallel molecular genetic study. We analysed shell morphology and anatomical traits and asked whether the clades are differentiated in these characters. In addition, the related species T. oreinos and T. striolatus were investigated and compared with the T. hispidus complex. Finally, we compared the ecological requirements of the taxa. Among the genetic clades of the T. hispidus complex there was no clear morphological differentiation and geographic populations could not be distinguished based on their morphology. The investigated characters of the genital anatomy did not allow discrimination of any of the T. hispidus clades and were not even diagnostic for the group as a whole. The morphotype of T. sericeus is present in all clades and thus cannot be assigned to a genetic group or any specific population. Thus, our morphological data do not provide evidence that any of the mitochondrial T. hispidus clades represent separate species. Concerning interspecific delimitation, the T. hispidus complex was clearly differentiated from T. striolatus and T. oreinos by shell morphological and anatomical characters, e.g. sculpture of shell surface and details of the penis. Finally, the habitat of T. oreinos is different from those of the other two species. In contrast to the lack of correspondence between genetic and morphological differentiation within the T. hispidus complex, related species display intraspecific morphological differentiation corresponding with mitochondrial clades: within T. striolatus there was a slight morphological differentiation between the subspecies T. s. striolatus, T. s. juvavensis and T. s. danubialis. The two subspecies of T. oreinos could be discriminated by a small but consistent difference in the cross-section of the penis. The unequal levels of intraspecific differentiation are caused by different evolutionary histories as a consequence of disparities in ecological demands, dispersal ability and use of glacial refugia: both the T. hispidus complex and T. striolatus are fast-spreading, euryoecious organisms which are able to (re-)colonize habitats and survive under different climate conditions. While the T. hispidus complex probably survived the Pleistocene in several glacial refugia, for T. striolatus one glacial refugium is suggested. Trochulus oreinos differs from the other taxa, as it is a slow disperser with a narrow ecological niche. We suggest that its subspecies spent at least the last glaciation in or close to the presently inhabited areas.

show abstract

“…Since morphological taxonomy relies on visible traits, it has been suggested that cryptic species may be more frequent when non-visual cues dominate intra-species interactions and hence selection for morphological traits is limited [8]. The soil is one such case, and analyses for springtails [9], oribatids [10] and molluscs [11,12] have all revealed a high degree of crypsis. Earthworms have high potential for local population isolation, and many named earthworm species are probably species complexes [13].…”

Section: Introductionmentioning

confidence: 99%

Identifying biochemical phenotypic differences between cryptic species

et al. 2014

View full text Add to dashboard Cite

Molecular genetic methods can distinguish divergent evolutionary lineages inwhat previously appeared to be single species, but it is not always clear what functional differences exist between such cryptic species. We used a metabolomic approach to profile biochemical phenotype (metabotype) differences between two putative cryptic species of the earthworm Lumbricus rubellus. There were no straightforward metabolite biomarkers of lineage, i.e. no metabolites that were always at higher concentration in one lineage. Multivariate methods, however, identified a small number of metabolites that together helped distinguish the lineages, including uncommon metabolites such as N1-trimethyllysine, which is not usually found at high concentrations. This approach could be useful for characterizing functional trait differences, especially as it is applicable to essentially any species group, irrespective of its genome sequencing status.

show abstract

A species delimitation approach in the Trochulus sericeus/hispiduscomplex reveals two cryptic species within a sharp contact zone

Cited by 53 publications

References 52 publications

Foot mucus stored on FTA® cards is a reliable and non-invasive source of DNA for genetics studies in molluscs

Foot mucus stored on FTA® cards is a reliable and non-invasive source of DNA for genetics studies in molluscs

Differentiation in the Trochulus hispidus complex and related taxa (Pulmonata: Hygromiidae): morphology, ecology and their relation to phylogeography

Identifying biochemical phenotypic differences between cryptic species

Contact Info

Product

Resources

About