Characterization of 1-aminocyclopropane-1-carboxylic acid synthase (ACS) genes during nectarine fruit development and ripening

Zeng, Wenfang; Pan, Lei; Liu, Hui; Niu, Liang; Lü, Zhenhua; Cui, Guochao; Wang, Zhiqiang

doi:10.1007/s11295-015-0833-6

Cited by 23 publications

(26 citation statements)

References 32 publications

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“…Ethylene production and flesh firmness were measured as described previously ( Zeng et al , 2015 ). After the measurement flesh firmness, part of the mesocarp was squeezed and SSC was measured with an Atago digital refractometer (Atago, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

PpYUC11, a strong candidate gene for the stony hard phenotype in peach (Prunus persicaL. Batsch), participates in IAA biosynthesis during fruit ripening

Pan

Zeng

Niu

et al. 2015

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Section: Methodsmentioning

confidence: 99%

PpYUC11, a strong candidate gene for the stony hard phenotype in peach (Prunus persicaL. Batsch), participates in IAA biosynthesis during fruit ripening

Pan

Zeng

Niu

et al. 2015

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113

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“…Flesh firmness and ethylene production were measured according to protocols described previously 40 . The ethylene concentration was measured with a GC2010 gas chromatograph (Shimadzu, Kyoto, Japan) equipped with a flame ionization detector.…”

Section: Measurement Of Ethylene Productionmentioning

confidence: 99%

“…The parameters for the remaining biochemical reactions were optimized on the basis of available proteomic and transcriptomic data on protein abundance and mRNA fold-changes derived from experimental measurements [YUC11, ACS1 mRNA fold-changes measured in; 24,40 MetE and ACO1 mRNA fold-changes measured in this study (Fig. 3)] and hormone production rates measured in the laboratory [IAA production rates at different stages were measured in 24 in the same cultivar, CN13; ethylene production rates were obtained in this study].…”

Section: Estimation Of Modeling Parameters For Quantificationmentioning

confidence: 99%

Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model

et al. 2020

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Peach (Prunus persica) is a typical climacteric fruit that produces ethylene rapidly during ripening, and its fruit softens quickly. Stony hard peach cultivars, however, do not produce large amounts of ethylene, and the fruit remains firm until fully ripe, thus differing from melting flesh peach cultivars. To identify the key proteins involved in peach fruit ripening, an antibody-based proteomic analysis was conducted. A mega-monoclonal antibody (mAb) library was generated and arrayed on a chip (mAbArray) at a high density, covering~4950 different proteins of peach. Through the screening of peach fruit proteins with the mAbArray chip, differentially expressed proteins recognized by 1587 mAbs were identified, and 33 corresponding antigens were ultimately identified by immunoprecipitation and mass spectrometry. These proteins included not only important enzymes involved in ethylene biosynthesis, such as ACO1, SAHH, SAMS, and MetE, but also novel factors such as NUDT2. Furthermore, protein-protein interaction analysis identified a metabolon containing SAHH and MetE. By combining the antibody-based proteomic data with the transcriptomic and metabolic data, a mathematical model of ethylene biosynthesis in peach was constructed. Simulation results showed that MetE is an important regulator during peach ripening, partially through interaction with SAHH.

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“…(pectins) of the cell walls, is a candidate gene for controlling the MF/NMF flesh texture [9][10][11]. SH fruits barely exhibit softening on the tree or after harvest and remain crisp due to a lack of ethylene production [12][13][14]. PpYUCCA11 (YUCCA Flavin mono-oxygenase gene), a candidate gene for the SH phenotype in peach, regulates auxin biosynthesis during peach fruit ripening [12,15].…”

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confidence: 99%

Genome-wide identification and transcriptome profiling reveal that E3 ubiquitin ligase genes relevant to ethylene, auxin and abscisic acid are differentially expressed in the fruits of melting flesh and stony hard peach varieties

Tan

Lian

Cheng

et al. 2019

Preprint

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Background: Ubiquitin ligases (E3) are the enzymes in the ubiquitin/26S proteasome pathway responsible for targeting proteins to the degradation pathway and play major roles in multiple biological activities. However, the E3 family and their functions are yet to be identified in the fruit of peach. Results: In this study, genome-wide identification, classification and characterization of the E3 ligase genes within the genome of peach (Prunus persica) was carried out. In total, 765 E3 (PpE3) ligase genes were identified in the peach genome. The PpE3 ligase genes were divided into eight subfamilies according to the presence of known functional domains. The RBX subfamily was not detected in peach. The PpE3 ligase genes were not randomly distributed among the 8 chromosomes, with a greater concentration on the longer chromosomes. The primary mode of gene duplication of the PpE3 ligase genes was dispersed gene duplication (DSD). Four subgroups of the BTB subfamily never characterized before were newly identified in peach, namely BTBAND, BTBBL, BTBP and BTBAN. The expression patterns of the identified E3 ligase genes in two peach varieties that display different types of fruit softening (melting flesh, MF, and stony hard, SH) were analyzed at 4 different stages of ripening using Illumina technology. Among the 765 PpE3 ligase genes, 515 (67.3%) were expressed (FPKM >1) in the fruit of either MF or SH during fruit ripening. In same-stage comparisons, 231 differentially expressed genes (DEGs) were identified between the two peach cultivars. The number of DEGs in each subfamily varied. Most DEGs were members of the BTB, F-box, U-box and RING subfamilies. PpE3 ligase genes predicted to be involved in ethylene, auxin, or ABA synthesis or signaling and DNA methylation were differentially regulated. Eight PpE3 ligase genes with possible roles in peach flesh texture and fruit ripening were discussed. Conclusions: The results of this study provide useful information for further understanding the functional roles of the ubiquitin ligase genes in peach. The findings also provide the first clues that E3 ligase genes may function in the regulation of peach ripening.

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Characterization of 1-aminocyclopropane-1-carboxylic acid synthase (ACS) genes during nectarine fruit development and ripening

Cited by 23 publications

References 32 publications

PpYUC11, a strong candidate gene for the stony hard phenotype in peach (Prunus persicaL. Batsch), participates in IAA biosynthesis during fruit ripening

PpYUC11, a strong candidate gene for the stony hard phenotype in peach (Prunus persicaL. Batsch), participates in IAA biosynthesis during fruit ripening

Application of an antibody chip for screening differentially expressed proteins during peach ripening and identification of a metabolon in the SAM cycle to generate a peach ethylene biosynthesis model

Genome-wide identification and transcriptome profiling reveal that E3 ubiquitin ligase genes relevant to ethylene, auxin and abscisic acid are differentially expressed in the fruits of melting flesh and stony hard peach varieties

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