PpYUC11, a strong candidate gene for the stony hard phenotype in peach (Prunus persicaL. Batsch), participates in IAA biosynthesis during fruit ripening

Pan, Lei; Zeng, Wenfang; Niu, Liang; Lü, Zhenhua; Liu, Hui; Cui, Guochao; Zhu, Yunqin; Chu, Jinfang; Li, Weiping; Fang, Weichao; Cai, Zuguo; Li, Guohuai; Wang, Zhiqiang

doi:10.1093/jxb/erv400

Cited by 91 publications

(116 citation statements)

References 65 publications

(82 reference statements)

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“…PpYUC11 was highly expressed in ripening ‘Akatsuki’ fruit, but it was not detectable in ‘Manami’. This result agrees with that of a previous study (Pan et al ., ). These results indicate that low PpYUC11 expression may be responsible for the IAA deficiency in ripening stony hard peaches.…”

Section: Resultsmentioning

confidence: 97%

“…In a previous study, diverse (TC)n (n is the repeat number) microsatellites were found in the first intron of PpYUC11 , and all stony hard peaches were homozygous for (TC) 20 [shown here as simple sequence repeat (SSR)20/20] (Pan et al ., ). We also investigated the SSR in the first intron of PpYUC11 of various cultivars.…”

Section: Resultsmentioning

confidence: 97%

“…The (TC) repeat numbers that were observed were 20, 25, 28 and 33, and the stony hard cultivars were SSR20/20 (Table ). Most genotypes of SSR20 coincided with the transposon insertion ( hd‐t ), and were consistent with the results of a previous report (Table , Pan et al ., ). However, the (TC) repeat number of the Hd genotype was not consistent with the result reported by Pan et al .…”

Section: Resultsmentioning

confidence: 97%

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Insertion of a transposon‐like sequence in the 5′‐flanking region of the YUCCA gene causes the stony hard phenotype

et al. 2018

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Melting-flesh peaches produce large amounts of ethylene, resulting in rapid fruit softening at the late-ripening stage. In contrast, stony hard peaches do not soften and produce little ethylene. The indole-3-acetic acid (IAA) level in stony hard peaches is low at the late-ripening stage, resulting in low ethylene production and inhibition of fruit softening. To elucidate the mechanism of low IAA concentration in stony hard peaches, endogenous levels of IAA and IAA intermediates or metabolites were analysed by ultra-performance liquid chromatography-tandem mass spectrometry. Although the IAA level was low, the indole-3-pyruvic acid (IPyA) level was high in stony hard peaches at the ripening stage. These results indicate that YUCCA activity is reduced in ripening stony hard peaches. The expression of one of the YUCCA isogenes in peach, PpYUC11, was suppressed in ripening stony hard peaches. Furthermore, an insertion of a transposon-like sequence was found upstream of the PpYUC11 gene in the 5'-flanking region. Analyses of the segregation ratio of the stony hard phenotype and genotype in F1 progenies indicated that the transposon-inserted allele of PpYUC11, hd-t, correlated with the stony hard phenotype. On the basis of the above findings, we propose that the IPyA pathway (YUCCA pathway) is the main auxin biosynthetic pathway in ripening peaches of 'Akatsuki' and 'Manami' cultivars. Because IAA is not supplied from storage forms, IAAde novo synthesis via the IPyA pathway (YUCCA pathway) in mesocarp tissues is responsible for auxin generation to support fruit softening, and its disruption can lead to the stony hard phenotype.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 97%

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Insertion of a transposon‐like sequence in the 5′‐flanking region of the YUCCA gene causes the stony hard phenotype

et al. 2018

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“…The SH trait (hdhd) is related to absence of ethylene production for a mutation in the Pp-ACS1 gene and altered indole-3-acetic acid levels due to regulation of PpYUC11 [6] [32] [33] [34]. In SH 7-28, homozygous-recessive for the stony hard gene (hdhd) [35], InDel analysis shows presence of the ~420 bp amplicon (Figure 2) attributable, by CAPS analysis, to a Pp-endoPG SH -like sequence ( Figure 6(c)).…”

Section: Discussionmentioning

confidence: 99%

Endopolygalacturonase Gene Polymorphisms: Asset of the Locus in Different Peach Accessions

Morgutti¹,

Negrini²,

Ghiani³

et al. 2017

AJPS

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“…Thus far, little is known about the role of PG family members in fruit softening. Pan et al (2015) indicated that PpPG21 ( ppa006839 ) and - 22 ( ppa006857 ) were relatively highly expressed in ripening peach fruit, but that their transcripts were almost undetectable in fruit during development [52]. Gu et al (2016) also demonstrated that PpPG22 and -21 are responsible for stone adhesion and melting flesh, respectively [25].…”

Section: Discussionmentioning

confidence: 99%

Identification and Expression Analysis of Polygalacturonase Family Members during Peach Fruit Softening

Qian

Zhang

Yan

et al. 2016

IJMS

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Polygalacturonase (PG) is an important hydrolytic enzyme involved in pectin degradation during fruit softening. However, the roles of PG family members in fruit softening remain unclear. We identified 45 PpPG genes in the peach genome which are clustered into six subclasses. PpPGs consist of four to nine exons and three to eight introns, and the exon/intron structure is basically conserved in all but subclass E. Only 16 PpPG genes were expressed in ripening fruit, and their expression profiles were analyzed during storage in two peach cultivars with different softening characteristics. Eight PGs (PpPG1, -10, -12, -13, -15, -23, -21, and -22) in fast-softening “Qian Jian Bai” (QJB) fruit and three PGs (PpPG15, -21, and -22) in slow-softening “Qin Wang” (QW) fruit exhibited softening-associated patterns; which also were affected by ethylene treatment. Our results suggest that the different softening characters in QW and QJB fruit is related to the amount of PG members. While keeping relatively lower levels during QW fruit softening, the expression of six PGs (PpPG1, -10, -12, -11, -14, and -35) rapidly induced by ethylene. PpPG24, -25 and -38 may not be involved in softening of peach fruit.

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PpYUC11, a strong candidate gene for the stony hard phenotype in peach (Prunus persicaL. Batsch), participates in IAA biosynthesis during fruit ripening

Abstract: Highlight PpYUC11 is a strong candidate gene for the control of the stony hard phenotype in peach.

Cited by 91 publications

References 65 publications

Insertion of a transposon‐like sequence in the 5′‐flanking region of the YUCCA gene causes the stony hard phenotype

Insertion of a transposon‐like sequence in the 5′‐flanking region of the YUCCA gene causes the stony hard phenotype

<i>Endopolygalacturonase</i> Gene Polymorphisms: Asset of the Locus in Different Peach Accessions

Identification and Expression Analysis of Polygalacturonase Family Members during Peach Fruit Softening

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PpYUC11, a strong candidate gene for the stony hard phenotype in peach (Prunus persicaL. Batsch), participates in IAA biosynthesis during fruit ripening

Abstract: Highlight PpYUC11 is a strong candidate gene for the control of the stony hard phenotype in peach.

Cited by 91 publications

References 65 publications

Insertion of a transposon‐like sequence in the 5′‐flanking region of the YUCCA gene causes the stony hard phenotype

Insertion of a transposon‐like sequence in the 5′‐flanking region of the YUCCA gene causes the stony hard phenotype

&lt;i&gt;Endopolygalacturonase&lt;/i&gt; Gene Polymorphisms: Asset of the Locus in Different Peach Accessions

Identification and Expression Analysis of Polygalacturonase Family Members during Peach Fruit Softening

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<i>Endopolygalacturonase</i> Gene Polymorphisms: Asset of the Locus in Different Peach Accessions