Characterization, mechanical behavior and in vitro evaluation of a melt-drawn scaffold for esophageal tissue engineering

Tan, Yu; Yeong, Wai Yee; Tan, Xipeng; An, Jia; Chian, Kerm Sin; Leong, Kah Fai

doi:10.1016/j.jmbbm.2015.12.015

Cited by 30 publications

(28 citation statements)

References 53 publications

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“…Relationship of zero-shear viscosities of PLC melt at different temperatures are shown in the inset. PLC melts at 110 °C [12] with a viscosity of 9889 Pa·s. It starts to flow when the temperature reaches 130 °C.…”

Section: Resultsmentioning

confidence: 99%

“…A model to empirically link the fiber diameter to the processing parameters was developed for the melt-drawing process [12]. The fiber diameter of PLC can be mathematically expressed in Equation (1) as:

normalD = {[\frac{\sqrt{2 gh}}{v} tanh (\frac{sans-serifρ normald \sqrt{2 gh}}{12 normalA} exp (\frac{- T_{g} {(T_{g} + 150)}^{2}}{0.164 normalT {(0.23 T_{g} + 150)}^{2}}))]}^{\frac{1}{2}}

where h = 50 mm,

ρ

= 1.215 g/cm 3 , d = 2 mm, T = 423.2 K and T g = 286.9 K [12] respectively. v represents the different melt-drawing speeds and A was calculated from Equations (2) and (3) to be 5.607 × 10 −5 Pa·s when η = 1245 Pa·s at 150 °C.…”

Section: Resultsmentioning

confidence: 99%

“…In this work, we propose a new melt-drawing method which requires only bench-top equipment and is less sensitive to external environmental effects. Melt-drawing of fiber can be used to fabricate polymeric tubular scaffolds in one step in a layer-by-layer manner based on the concept of additive manufacturing (AM) [11,12]. AM has been demonstrated as a viable technique in 3D printing of biocompatible materials such as metals (e.g., titanium [13,14] and stainless steel [15]), ceramics [16] and polymers [17] for biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

“…Since most of the tubular organs exhibit certain elasticity for fluid and/or food bolus flow, elastic PLC is suitable to resemble them. PLC was used in our previous papers [11,12] for esophageal TE and it was shown to have tensile properties comparable to the native esophagus.…”

Section: Introductionmentioning

confidence: 99%

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Additive Manufacturing of Patient-Customizable Scaffolds for Tubular Tissues Using the Melt-Drawing Method

Tan

Yeong

et al. 2016

Materials

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Polymeric fibrous scaffolds for guiding cell growth are designed to be potentially used for the tissue engineering (TE) of tubular organs including esophagi, blood vessels, tracheas, etc. Tubular scaffolds were fabricated via melt-drawing of highly elastic poly(l-lactide-co-ε-caprolactone) (PLC) fibers layer-by-layer on a cylindrical mandrel. The diameter and length of the scaffolds are customizable via 3D printing of the mandrel. Thickness of the scaffolds was varied by changing the number of layers of the melt-drawing process. The morphology and tensile properties of the PLC fibers were investigated. The fibers were highly aligned with a uniform diameter. Their diameters and tensile properties were tunable by varying the melt-drawing speeds. These tailorable topographies and tensile properties show that the additive-based scaffold fabrication technique is customizable at the micro- and macro-scale for different tubular tissues. The merits of these scaffolds in TE were further shown by the finding that myoblast and fibroblast cells seeded onto the scaffolds in vitro showed appropriate cell proliferation and distribution. Human mesenchymal stem cells (hMSCs) differentiated to smooth muscle lineage on the microfibrous scaffolds in the absence of soluble induction factors, showing cellular shape modulation and scaffold elasticity may encourage the myogenic differentiation of stem cells.

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Section: Resultsmentioning

confidence: 99%

normalD = {[\frac{\sqrt{2 gh}}{v} tanh (\frac{sans-serifρ normald \sqrt{2 gh}}{12 normalA} exp (\frac{- T_{g} {(T_{g} + 150)}^{2}}{0.164 normalT {(0.23 T_{g} + 150)}^{2}}))]}^{\frac{1}{2}}

where h = 50 mm,

ρ

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Additive Manufacturing of Patient-Customizable Scaffolds for Tubular Tissues Using the Melt-Drawing Method

Tan

Yeong

et al. 2016

Materials

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“…This can be attributed to the random distribution of the isotropic microspheres. It is suggested that cell-laden microfibrils or microfibers45 can be used in bioprinting to provide anisotropic alignment when an aligned tissue is needed such as with neural46 and gastrointestinal tract tissues4748.…”

Section: Discussionmentioning

confidence: 99%

Hybrid microscaffold-based 3D bioprinting of multi-cellular constructs with high compressive strength: A new biofabrication strategy

Tan

Yeong

et al. 2016

Sci Rep

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106

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A hybrid 3D bioprinting approach using porous microscaffolds and extrusion-based printing method is presented. Bioink constitutes of cell-laden poly(D,L-lactic-co-glycolic acid) (PLGA) porous microspheres with thin encapsulation of agarose-collagen composite hydrogel (AC hydrogel). Highly porous microspheres enable cells to adhere and proliferate before printing. Meanwhile, AC hydrogel allows a smooth delivery of cell-laden microspheres (CLMs), with immediate gelation of construct upon printing on cold build platform. Collagen fibrils were formed in the AC hydrogel during culture at body temperature, improving the cell affinity and spreading compared to pure agarose hydrogel. Cells were proven to proliferate in the bioink and the bioprinted construct. High cell viability up to 14 days was observed. The compressive strength of the bioink is more than 100 times superior to those of pure AC hydrogel. A potential alternative in tissue engineering of tissue replacements and biological models is made possible by combining the advantages of the conventional solid scaffolds with the new 3D bioprinting technology.

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Biofabrication of an Esophageal Tissue Construct from a Polymer Blend Using 3D Extrusion‐Based Printing

et al. 2022

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Many tissue engineering approaches are being explored to offer solutions for diseased esophageal tissue and its repair. However, classical techniques in tissue engineering are not capable of recapitulating the structural and functional parameters of native esophagi. Esophageal 3D bioprinting is yet an emerging field but holds great promise in meeting this challenge. Herein, the use of extrusion‐based 3D printing is examined to generate esophageal substitutes from a polymer blend‐based formulation. The fabricated 3D esophageal structures are printed at a very high speed without collapse and without the use of supporting material. In vitro analysis reveals that the printed material enables cell adhesion, proliferation, and migration into the deeper zones. Moreover, the designed construct is suturable to the native esophagus and exhibits no leakage while offering mechanical properties similar to that of the native tissue. Overall, these biochemical and biomechanical features make the reported artificial esophagus a promising solution for the repair of damaged esophagi.

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Characterization, mechanical behavior and in vitro evaluation of a melt-drawn scaffold for esophageal tissue engineering

Cited by 30 publications

References 53 publications

Additive Manufacturing of Patient-Customizable Scaffolds for Tubular Tissues Using the Melt-Drawing Method

Additive Manufacturing of Patient-Customizable Scaffolds for Tubular Tissues Using the Melt-Drawing Method

Hybrid microscaffold-based 3D bioprinting of multi-cellular constructs with high compressive strength: A new biofabrication strategy

Biofabrication of an Esophageal Tissue Construct from a Polymer Blend Using 3D Extrusion‐Based Printing

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