Characterization, expression profiling, and functional identification of a gene encoding geranylgeranyl diphosphate synthase from Salvia miltiorrhiza

Kai, Guoyin; Liao, Pan; Zhang, Tong; Zhou, Wei; Wang, Jing; Xu, Hui; Liu, Yuanyun; Zhang, Lin

doi:10.1007/s12257-009-0123-y

Cited by 54 publications

(36 citation statements)

References 27 publications

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“…The results showed that the putative SmHMGS was differentially expressed in various tissues and was found to be highest in leaf followed by stem and root (Fig. 4), which was similar to SmDXR and SmGGPPS expression Kai et al 2010), whereas was contrary to SmHMGR. SmHMGR showed the highest expression in roots, while weak expression was detected in leafs ).…”

Section: Expression Analysis Of the Putative Smhmgs In Different Tissmentioning

confidence: 85%

“…Plant treatment and total RNA isolation RNA of the leaves from S. miltiorrhiza plants was extracted using the method reported previously Liao et al 2009;Kai et al 2010). 4-week-old seedlings of S. miltiorrhiza were spayed with the solution of 50 lM MJ and 10 mg/L SA, respectively, with water as control for each treatment.…”

Section: Methodsmentioning

confidence: 99%

“…In the comparison of SmHMGR ), the expression of the putative SmHMGS is much higher than SmHMGR by comparing the expression of 18s rRNA. What is impressive is that the four genes including SmHMGS, SmHMGR, SmDXR and SmGGPPS showed the highest expression level at 48 h after SA induction treatment Yan et al 2009;Kai et al 2010).…”

Section: Expression Analysis Of the Putative Smhmgs Under Induction Omentioning

confidence: 96%

“…However, until now, there is limited information about the mRNA expression profile of SmHMGS either in different plant tissues or under various kinds of elicitor treatments. Several genes, such as HMGR, DXR and GGPPS involved in tanshinones biosynthetic pathway from S. miltiorrhiza have been isolated by our laboratory recently Yan et al 2009;Kai et al 2010). It is also meaningful to explore expression profiles of SmHMGS and compare it with the above isolated genes involved in tanshinones biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and expression analysis of a new putative gene encoding 3-hydroxy-3-methylglutaryl-CoA synthase from Salvia miltiorrhiza

et al. 2010

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A new putative gene encoding 3-hydroxy-3-methylglutaryl coenzyme A synthase (designated as SmHMGS, GenBank Accession No. FJ785326), which catalyses the condensation of acetyl-CoA and acetoacetylCoA to form 3-hydroxy-3-methylglutaryl-CoA as an early step in the mevalonic acid pathway, was isolated from young leaves of Salvia miltiorrhiza by rapid amplification of cDNA ends (RACE) for the first time. The full-length cDNA of the putative SmHMGS was 1,655 bp containing a 1,381 bp open reading frame (ORF) encoding a polypeptide of 460 amino acids. Comparative and bioinformatic analyses revealed that SmHMGS showed extensive homology with HMGSs from other plant species. Phylogenetic tree analysis indicated that SmHMGS belonged to the plant HMGS super family and had the closest relationship with HMGS from Hevea brasiliensis. Tissue expression pattern analysis revealed that the putative SmHMGS was constitutively expressed in all the tested tissues and strong in leaf, moderate in stem, weak in root, which was in contrast to SmHMGR reported before. The putative SmHMGS was found to be an elicitor-responsive gene, which could be induced by exogenous elicitors, including salicylic acid (SA) and methyl jasmonate (MJ).These results will help in understanding the role of HMGS in tanshinones biosynthesis in S. miltiorrhiza.

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Section: Expression Analysis Of the Putative Smhmgs In Different Tissmentioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Section: Expression Analysis Of the Putative Smhmgs Under Induction Omentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

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Molecular cloning and expression analysis of a new putative gene encoding 3-hydroxy-3-methylglutaryl-CoA synthase from Salvia miltiorrhiza

et al. 2010

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“…A series of genes involved in tanshinone biosynthetic pathway has been cloned and its function identified, such as 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR), which is the initial and rate-limiting enzyme in the MVA pathway ); 1-deoxy-D-xylulose-5-phosphate reductoisomerase (DXR) plays a key role in the committed steps of the DXP pathway ). Isopentenyl diphosphate isomerase (IPPI) and geranylgeranyl diphosphate synthase (GGPPS) are also important in the midstream of the whole pathway (Kai et al 2010;Ma et al 2015). Nevertheless, tanshinone biosynthetic pathway that existed in S. castanea Diels f. tomentosa Stib.…”

Section: Introductionmentioning

confidence: 99%

Establishment of Salvia castanea Diels f. tomentosa Stib. hairy root cultures and the promotion of tanshinone accumulation and gene expression with Ag+, methyl jasmonate, and yeast extract elicitation

Wang

et al. 2015

Protoplasma

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Salvia castanea Diels f. tomentosa Stib. is an endemic medicinal plant distributed in China, and the notably high content of tanshinone IIA in the root is proven effective for the therapy of heart diseases. Hairy root induction of this Salvia species was inoculated with Agrobacterium rhizogenes strain ATCC 15834. Transformed hairy root was cultured in 6,7-V liquid medium for growth kinetics assessment and elicitation. An S curve was present in the hairy root cultures based on the fresh and dry weights with an interval of 3 days. An optimum concentration of the applied elicitors (15 μM Ag(+), 200 μM methyl jasmonate, and 200 mg l(-1) yeast extract elicitor) benefitted both the growth status and tanshinone accumulation in the hairy root cultures. Tanshinone IIA contents were mostly stimulated 1.8-fold and 1.99-fold compared with the control by Ag(+) and methyl jasmonate elicitation, respectively. Yeast extract dramatically enhanced dry mass accumulation, while it promoted cryptotanshinone content of 2.84 ± 0.33 mg g(-1) dry weight at most in the hairy root cultures. Selected elicitors diversely influenced tanshinone accumulation in the time courses of hairy root cultures within 7 days. Furthermore, transcripts of selected genes in the tanshinone biosynthetic pathway were remarkably upregulated with elicitation. Yeast extract elicitor heightened 13.9-fold of isopentenyl diphosphate isomerase expression level at 12 h, while it increased 16.7-fold of geranylgeranyl diphosphate synthase transcript at 24 h compared with that of the control, which was more effective than Ag(+) and methyl jasmonate. This study provided a convenient hairy root culture system of S. castanea Diels f. tomentosa Stib. for tanshinone production for the first time.

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Effects of methyl jasmonate and salicylic acid on tanshinone production and biosynthetic gene expression in transgenic Salvia miltiorrhiza hairy roots

Hao

Shi

Cui

et al. 2014

Biotech and App Biochem

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108

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Tanshinone is a group of active diterpenes, which are widely used in the treatment of cardiovascular disease. In this study, methyl jasmonate (MJ) and salicylic acid (SA) were used to investigate their effects on tanshinone accumulation and biosynthetic gene expression in the hairy roots of geranylgeranyl diphosphate synthase (SmGGPPS) overexpression line (G50) in Salvia miltiorrhiza. High-performance liquid chromatography analysis showed that total tanshinone content in G50 was obviously increased by 3.10-fold (11.33 mg/g) with MJ at 36 H and 1.63 times (5.95 mg/g) after SA treatment for 36 H in comparison with their mimic treatment control. Furthermore, quantitative reverse-transcription PCR analysis showed that the expression of isopentenyl-diphosphate delta-isomerase (SmIPPI), SmGGPPS, copalyl diphosphate synthase (SmCPS), and kaurene synthase-like (SmKSL) increased significantly with MJ treatment. However, the expression of SmIPPI reached the highest level at 144 H, whereas those of SmGGPPS, SmCPS, and SmKSL only increased slightly with SA treatment. The two elicitor treatments suggested that tanshinone accumulation positively correlated to the expression of key genes such as SmGGPPS, SmCPS, and SmKSL. Meanwhile, the study also indicated that it was a feasible strategy to combine elicitor treatment with transgenic technology for the enhancement of tanshinone, which paved the way for further metabolic engineering of tanshinone biosynthesis.

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Characterization, expression profiling, and functional identification of a gene encoding geranylgeranyl diphosphate synthase from Salvia miltiorrhiza

Cited by 54 publications

References 27 publications

Molecular cloning and expression analysis of a new putative gene encoding 3-hydroxy-3-methylglutaryl-CoA synthase from Salvia miltiorrhiza

Molecular cloning and expression analysis of a new putative gene encoding 3-hydroxy-3-methylglutaryl-CoA synthase from Salvia miltiorrhiza

Establishment of Salvia castanea Diels f. tomentosa Stib. hairy root cultures and the promotion of tanshinone accumulation and gene expression with Ag+, methyl jasmonate, and yeast extract elicitation

Effects of methyl jasmonate and salicylic acid on tanshinone production and biosynthetic gene expression in transgenic Salvia miltiorrhiza hairy roots

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