Effects of methyl jasmonate and salicylic acid on tanshinone production and biosynthetic gene expression in transgenic Salvia miltiorrhiza hairy roots

Abstract

Tanshinone is a group of active diterpenes, which are widely used in the treatment of cardiovascular disease. In this study, methyl jasmonate (MJ) and salicylic acid (SA) were used to investigate their effects on tanshinone accumulation and biosynthetic gene expression in the hairy roots of geranylgeranyl diphosphate synthase (SmGGPPS) overexpression line (G50) in Salvia miltiorrhiza. High-performance liquid chromatography analysis showed that total tanshinone content in G50 was obviously increased by 3.10-fold (11.33 mg/g) with MJ at 36 H and 1.63 times (5.95 mg/g) after SA treatment for 36 H in comparison with their mimic treatment control. Furthermore, quantitative reverse-transcription PCR analysis showed that the expression of isopentenyl-diphosphate delta-isomerase (SmIPPI), SmGGPPS, copalyl diphosphate synthase (SmCPS), and kaurene synthase-like (SmKSL) increased significantly with MJ treatment. However, the expression of SmIPPI reached the highest level at 144 H, whereas those of SmGGPPS, SmCPS, and SmKSL only increased slightly with SA treatment. The two elicitor treatments suggested that tanshinone accumulation positively correlated to the expression of key genes such as SmGGPPS, SmCPS, and SmKSL. Meanwhile, the study also indicated that it was a feasible strategy to combine elicitor treatment with transgenic technology for the enhancement of tanshinone, which paved the way for further metabolic engineering of tanshinone biosynthesis.

Five‐descriptor model to predict the chromatographic sequence of natural compounds

“…The case of Radix Salvia miltiorrhiza was used for demonstrating the potential application of this method. Substantial evidence has shown that Tanshinone IIA is one of the main pharmacologically active ingredients in Radix Salvia miltiorrhiza . Our radar chart analysis suggests that Tanshinone IIA is a typical strong retention compound in the C 18 column (Supporting Information Fig.…”

Transcriptome Analysis of Salicylic Acid Treatment in Rehmannia glutinosa Hairy Roots Using RNA-seq Technique for Identification of Genes Involved in Acteoside Biosynthesis

“…In this report, we found that SA and MeJA could increase accumulation of acteoside in R. glutinosa hairy roots, but SA was much more effective than MeJA in stimulating the accumulation of acteoside (Figure 1B). SA is a well-known inducer of plant systematic acquired resistance in plant-pathogen (Jian et al, 2005), and often used to improve the accumulation of plant secondary metabolites (Hao et al, 2014; Saini et al, 2014). The content of total PeG of C. deserticola cell cultures was obviously elevated by SA at concentration range of 10 ~ 100 μmol/L (Xu et al, 2005).…”

Transcriptome Analysis of Genes Associated with the Artemisinin Biosynthesis by Jasmonic Acid Treatment under the Light in Artemisia annua

“…Jasmonic acid (JA) can regulate the secondary metabolism in several plant species (De Geyter et al, 2012; Zhou and Memelink, 2016), including some traditional Chinese medicinal plants, like Scutellaria baicalensis (Zhao et al, 2016), Salvia miltiorrhiza (Hao et al, 2015), and Catharanthus roseus (van der Fits and Memelink, 2000). It is reported that the JA treatment could simultaneously stimulate artemisinin biosynthesis and promote the formation of glandular trichomes in A. annua (Maes et al, 2011).…”