Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species

Fadel, Hanaa; Afifi, Rabab; Al-Qabili, Dheyazan Mohammed

doi:10.14202/vetworld.2017.1118-1128

Cited by 12 publications

(13 citation statements)

References 45 publications

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“…Shiga toxin-producing E. coli have been largely detected in domestic and wild animals, including gulls [ 7 , 14 ]. Although the prevalence was lower, as compared to other surveys conducted on gulls across Europe [ 12 ], the STEC percentage is in line with other studies conducted on wild birds recovered in urban surroundings [ 34 , 35 ]. Unfortunately, it was not possible to establish the O:H serotypes, in order to determine the seropathotypes according to Karmali et al [ 36 ].…”

Section: Discussionsupporting

confidence: 88%

Prevalence and Antimicrobial Resistance of Enteropathogenic Bacteria in Yellow-Legged Gulls (Larus michahellis) in Southern Italy

Russo

Pace

Varriale

et al. 2021

Animals

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Wild birds may host and spread pathogens, integrating the epidemiology of infectious diseases. Particularly, Larus spp. have been described as responsible for the spread of many enteric diseases, primarily because of their large populations at landfill sites. The aim of this study was to examine the role of yellow-legged gulls as a source of enteropathogenic bacteria such as Campylobacter spp., Salmonella spp., Shiga toxin-producing Escherichia coli and Yersinia spp., with particular attention to antibiotic-resistant strains. Enteropathogenic bacteria were isolated from 93/225 yellow-legged gulls examined from April to July, during a four-year period (2016–2019). Specifically, Campylobacter spp. was isolated from 60/225 samples (26.7%), and identified as C. coli (36/60) and as C. jejuni (24/60). Salmonella spp. was isolated from 3/225 samples (1.3%), and identified as Salmonella arizonae. Shiga toxin-producing E. coli were isolated from 30/225 samples (13.3%) samples, and serotyped as E. coli O128 (12/30) O26 (9/30), O157 (6/30) and O11 (3/30); Yersinia spp. was never detected. Isolated strains exhibited multidrug resistance, including vitally important antibiotics for human medicine (i.e., fluoroquinolones, tetracyclines). Our study emphasizes the importance of yellow-legged gulls as potential reservoirs of pathogenic and resistant strains and their involvement in the dissemination of these bacteria across different environments, with resulting public health concerns.

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Section: Discussionsupporting

confidence: 88%

Prevalence and Antimicrobial Resistance of Enteropathogenic Bacteria in Yellow-Legged Gulls (Larus michahellis) in Southern Italy

Russo

Pace

Varriale

et al. 2021

Animals

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“…The occurrence among the examined ducks was 0.48%, while STEC was detected only among Anas crecca duck species with an occurrence rate 0.92%, with such result was lower than that obtained by Fadel et al . in Egypt, who isolated STEC with occurrence rate 10% (1/10) among the examined Anas crecca [ 30 ] and Bertelloni et al . in Italy, who recovered STEC with occurrence rate 4.5% (1/22) [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…[ 13 ] while Fadel et al . [ 30 ] found both stx1 and stx2 genes in only one positive duck sample. On the other hand, the occurrence of STEC among the examined migratory quails was 0.71%, which is considered lower than that found by Dipineto et al .…”

Section: Discussionmentioning

confidence: 99%

Migratory birds as a potential overseas transmitter of Shiga toxin-producing Escherichia coli

Seleem

Sabry

Abdel-Moein

2021

International Journal of Veterinary Science and Medicine

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Shiga toxin-producing Escherichia coli (STEC) has a great public health importance. This study was conducted to investigate the potential role of migratory birds in the transmission of STEC. For this purpose, cloacal swabs were collected from 349 migratory birds (209 ducks and 140 quails) from Damietta governorate, Egypt. The collected swabs were cultured for isolation of STEC using the STEC CHROMagar. STEC isolates were identified based on colonial characteristics, Gram’s stain, conventional biochemical tests and molecular detection of stx1, stx2 and eae genes. Positive isolates were serotyped and examined for their antibiotic susceptibility pattern. Furthermore, gene sequencing was performed for genes stx1 and stx2 . Of the examined birds, two STEC isolates were a obtained with an overall occurrence rate 0.57% (2/349), one isolate carried stx2 gene from a migratory quail 0.71% (1/140), and another isolate from a migratory duck carried stx1 gene 0.48% (1/209), whereas both isolates were negative for eae gene. Moreover, the duck isolate was serotyped O86, while the quail isolate was serotyped O125; both isolates were multidrug resistant. The phylogenetic analysis of the obtained stx1 and stx2 genes revealed high genetic relatedness to those isolated from human cases in the countries where such birds either lived or were in their migratory pathway. In conclusion, this study highlights the potential role of migratory birds in transmitting multidrug-resistant STEC across their migratory pathway.

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“…It has been shown that birds can harbor STEC, but, like cattle, they seem refractory to Stx and could be a spillover host. Starlings and pigeons would be likely culprits in a spillover scenario because these two avian species can inhabit city buildings, parks and playgrounds, where humans may be exposed to these animals and their feces [ 28 , 29 , 30 ]. Wild birds were first identified as a potential source of STEC infection in 1997 [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Two Paralogous Gb3/CD77 Synthases in Birds Show Different Preferences for Their Glycoprotein and Glycosphingolipid Substrates

Bereznicka

Mikołajczyk

Szymczak-Kulus

et al. 2021

IJMS

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Most glycosyltransferases show remarkable gross and fine substrate specificity, which is reflected in the old one enzyme-one linkage paradigm. While human Gb3/CD77 synthase is a glycosyltransferase that synthesizes the Galα1→4Gal moiety mainly on glycosphingolipids, its pigeon homolog prefers glycoproteins as acceptors. In this study, we characterized two Gb3/CD77 synthase paralogs found in pigeons (Columba livia). We evaluated their specificities in transfected human teratocarcinoma 2102Ep cells by flow cytofluorometry, Western blotting, high-performance thin-layer chromatography, mass spectrometry and metabolic labelling with 14C-galactose. We found that the previously described pigeon Gb3/CD77 synthase (called P) can use predominately glycoproteins as acceptors, while its paralog (called M), which we serendipitously discovered while conducting this study, efficiently synthesizes Galα1→4Gal caps on both glycoproteins and glycosphingolipids. These two paralogs may underlie the difference in expression profiles of Galα1→4Gal-terminated glycoconjugates between neoavians and mammals.

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Characterization and zoonotic impact of Shiga toxin producing Escherichia coli in some wild bird species

Cited by 12 publications

References 45 publications

Prevalence and Antimicrobial Resistance of Enteropathogenic Bacteria in Yellow-Legged Gulls (Larus michahellis) in Southern Italy

Prevalence and Antimicrobial Resistance of Enteropathogenic Bacteria in Yellow-Legged Gulls (Larus michahellis) in Southern Italy

Migratory birds as a potential overseas transmitter of Shiga toxin-producing Escherichia coli

Two Paralogous Gb3/CD77 Synthases in Birds Show Different Preferences for Their Glycoprotein and Glycosphingolipid Substrates

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