Characterization and Therapy for Experimental Infection by Helicobacter mustelae in Ferrets

Czinn, Steven J.; Bierman, John C.; Diters, R. W.; Blanchard, Thomas G.; Leunk, R D

doi:10.1111/j.1523-5378.1996.tb00007.x

Cited by 13 publications

(7 citation statements)

References 22 publications

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“…This finding suggests that developing an immune response before challenge is necessary to mediate protection. However, animals successfully treated with antibiotics to eradicate helicobacters are not resistant to reinfection, despite the immune response generated in response to the primary infection (7,13,14). In addition, several studies performed in both the mouse and ferret models of helicobacter infection demonstrate that therapeutic immunization of infected animals results in the eradication of the bacteria from the gastric mucosa (8,9,15).…”

Section: Discussionmentioning

confidence: 99%

Local and Systemic Antibody Responses in Humans withHelicobacter pyloriInfection

Blanchard

Nedrud²,

Czinn³

1999

Canadian Journal of Gastroenterology

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Immunization can prevent or cure an otherwise chronic helicobacter infection in several animal models despite the chronic nature of natural helicobacter infections. Differences in the antigenic specificity of the antibodies may contribute to the protection observed in these experimental animals. The goal of the present study was to compare the local and systemic antibody responses of humans with chronic Helicobacter pylori infection with those of an individual with spontaneous resolution of infection to find an immunological correlate of protection. Spontaneous resolution of infection was accompanied by a change in immunoblot profiles. Whereas a broad range of H pylori antigens was recognized in chronically infected patients (including the patient who ultimately cleared the infection spontaneously), resolution of infection in the absence of therapeutic agents resulted in the recognition of only several immunodominant antigens. The most dominant antigen was approximately 66 kDa in molecular mass. Immunoblot analysis demonstrated that these antibodies were specific for the structural subunits of the urease enzyme. These studies suggest that the success of antihelicobacter immunization may be due to the ability of vaccination to induce an immune response against antigens that are normally not immunodominant during the course of infection.

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Section: Discussionmentioning

confidence: 99%

Local and Systemic Antibody Responses in Humans withHelicobacter pyloriInfection

Blanchard

Nedrud²,

Czinn³

1999

Canadian Journal of Gastroenterology

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“…Because of notable interest in development of a vaccine that would prevent or eliminate coloniza- tion of the gastric mucosa with H. pylori , several animal models of Helicobacter infection and pathogenesis have been investigated for utility in vaccine and therapeutic protocols [10][11][12][13][14][15][16]. Clearly, the immune response to H. mustelae induced by natural exposure, as is true for H. pylori in humans, is incapable of eliminating active infection or preventing reinfection after antibiotic therapy [17,18]. Therefore, a compelling need exists for establishing effective oral adjuvants to stimulate a protective mucosal immune response.…”

mentioning

confidence: 99%

Promotion of Ulcerative Duodenitis in Young Ferrets by Oral Immunization with Helicobacter mustelae and Muramyl Dipeptide

et al. 1997

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Oral vaccination of young ferrets with Hml and 50 micrograms MDP increased the risk of Helicobacter-associated mucosal ulceration in the proximal duodenum, which was associated with low humoral (but significant cell-mediated) immune responses to H. mustelae. In retrospect, the frequency of vaccination may have suppressed the systemic humoral immune response, thereby promoting mucosal damage by H. mustelae. The 50-microgram dose of MDP enhanced the cell-mediated immune response, which indirectly contributed to development of severe lesions. The increased frequency of mucosal damage associated with this vaccination regimen enhances the value of the ferret model for studying duodenal ulceration secondary to Helicobacter infection.

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“…Furthermore, emergence of antibiotic resistant H. pylori is relatively common. Finally, studies in animal models indicate that although antibiotics can cure Helicobacter infections, immunity to re‐infection does not develop [8–10]. Thus, in the early 1990s we and others began to investigate vaccination as an alternate therapy for control of H. pylori infections.…”

Section: Rationale For An H Pylori Vaccinementioning

confidence: 99%

Helicobacter pylori Vaccines: Lessons from Small Animal Models

Nedrud

2001

Scand J Immunol

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Characterization and Therapy for Experimental Infection by Helicobacter mustelae in Ferrets

Cited by 13 publications

References 22 publications

Local and Systemic Antibody Responses in Humans withHelicobacter pyloriInfection

Local and Systemic Antibody Responses in Humans withHelicobacter pyloriInfection

Promotion of Ulcerative Duodenitis in Young Ferrets by Oral Immunization with Helicobacter mustelae and Muramyl Dipeptide

Helicobacter pylori Vaccines: Lessons from Small Animal Models

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