Promotion of Ulcerative Duodenitis in Young Ferrets by Oral Immunization with <i>Helicobacter mustelae</i> and Muramyl Dipeptide

Whary, Mark T.; Palley, Lori S.; Batchelder, Margaret; Murphy, James C.; Yan, Lili; Taylor, Nancy S.; Fox, James G.

doi:10.1111/j.1523-5378.1997.tb00061.x

Cited by 22 publications

(13 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The high anti-H. cetorum IgG levels in these dolphins is consistent with observations made in ferrets in which 100% were found to be infected with H. mustelae, with seroconversion occurring soon after weaning (26), and the correlation of positive serology and prevalence of H. pylori infection in humans, which varies from low to 90% or higher in some populations (19). Serology kits used for screening humans for H. pylori infection have reported a median sensitivity and specificity of 92 and 83%, respectively (22).…”

Section: Fig 3 (A)supporting

confidence: 90%

Comparison of Diagnostic Techniques forHelicobacter cetorumInfection in Wild Atlantic Bottlenose Dolphins (Tursiops truncatus)

et al. 2003

Self Cite

View full text Add to dashboard Cite

Helicobacter cetorum sp. nov. has been cultured from the stomach of Atlantic white-sided dolphins (Lagenorhynchus acutus) and the feces of Pacific white-sided (L. obliquidens) and Atlantic bottlenose (Tursiops truncatus) dolphins and a beluga whale (Delphinapterus leucas). H. cetorum has high homology to Helicobacter pylori as shown by 16S rRNA sequencing, and H. cetorum infection has been associated with gastritis and clinical signs in cetaceans. Because the prevalence of H. cetorum in wild populations is unknown, minimally invasive techniques for detecting H. cetorum were compared for 20 wild bottlenose dolphins sampled as part of a long-term health study. Fecal samples were tested for helicobacter by culture, Southern blotting, and PCR using genus-specific and H. cetorum-specific primers. An enzyme-linked immunosorbent assay (ELISA) was developed to measure H. cetorum immunoglobulin G (IgG). H. cetorum was cultured from 4 of 20 fecal samples, 7 samples were positive using Helicobacter sp. PCR, and 8 samples were positive for H. cetorum using speciesspecific primers. Two additional fecal samples were positive by Helicobacter sp. Southern blotting, suggesting infection with another helicobacter. All 20 sera contained high levels of IgG antibodies to H. cetorum that were significantly lowered by preabsorption of the sera with whole-cell suspensions of H. cetorum (P < 0.02). Until the specificity of the serum ELISA can be determined by testing sera from dolphins confirmed to be uninfected, PCR and Southern blot screenings of feces are the most sensitive techniques for detection of H. cetorum, and results indicate there is at least a 50% prevalence of H. cetorum infection in these dolphins.

show abstract

Section: Fig 3 (A)supporting

confidence: 90%

Comparison of Diagnostic Techniques forHelicobacter cetorumInfection in Wild Atlantic Bottlenose Dolphins (Tursiops truncatus)

et al. 2003

Self Cite

View full text Add to dashboard Cite

show abstract

“…When ferrets naturally infected with H. mustelae were immunized with H. pylori urease, colonization was eliminated in only 30% of animals (60). Immunization of uninfected ferret kits with H. mustelae lysate, together with the adjuvant muramyl dipeptide, was also ineffective at primary prevention (422). These disappointing results may be attributed to many variables in addition to the fact that H. mustelae naturally infects the ferret.…”

Section: Helicobacter Felismentioning

confidence: 91%

Emergence of DiverseHelicobacterSpecies in the Pathogenesis of Gastric and Enterohepatic Diseases

2001

View full text Add to dashboard Cite

Since Helicobacter pylori was first cultivated from human gastric biopsy specimens in 1982, it has become apparent that many related species can often be found colonizing the mucosal surfaces of humans and other animals. These other Helicobacter species can be broadly grouped according to whether they colonize the gastric or enterohepatic niche. Gastric Helicobacter species are widely distributed in mammalian hosts and are often nearly universally prevalent. In many cases they cause an inflammatory response resembling that seen with H. pylori in humans. Although usually not pathogenic in their natural host, these organisms serve as models of human disease. Enterohepatic Helicobacter species are an equally diverse group of organisms that have been identified in the intestinal tract and the liver of humans, other mammals, and birds. In many cases they have been linked with inflammation or malignant transformation in immunocompetent hosts and with more severe clinical disease in immunocompromised humans and animals. The purpose of this review is to describe these other Helicobacter species, characterize their role in the pathogenesis of gastrointestinal and enterohepatic disease, and discuss their implications for our understanding of H. pylori infection in humans

show abstract

“…Muramyl dipeptide (N‐aetylmuramyl‐ L ‐alanyl‐ D ‐isoglutamine) has been tested in therapeutic immunization of H. mustelae infected ferrets, with infected animals being dosed three times in 5 days and then weekly for 7 further weeks. No effect on bacterial colonization was observed, although as the authors pointed out, this may have been due to excessive numbers of immunizations 18 . In our experience with prophylactic immunization of mice against H. pylori , four weekly immunizations is less effective than two immunizations given 3 weeks apart 19 .…”

Section: Adjuvantsmentioning

confidence: 84%

Helicobacter pylori vaccines—the current status

Sutton

Lee

2000

Aliment Pharmacol Ther

View full text Add to dashboard Cite

In this review, we take a look at the current status in the development of a vaccine against the human pathogenic bacterium, Helicobacter pylori, a major aetiological factor in peptic ulcer disease and gastric adenocarcinoma. Various animal models are now in use from mice infected with H. pylori, through gnotobiotic pigs and primates to ferrets naturally infected with their own Helicobacter, H. mustelae. A significant problem remains the requirement for a suitable mucosal adjuvant. Detoxification or the use of low doses of adjuvants already available may provide a solution and new immune stimulating compounds have been tested with some success. New approaches include the delivery of Helicobacter antigens by DNA immunization, microparticles or live vectors such as attenuated salmonella and the examination of alternative routes of vaccine administration. The phenomenon of post-immunization gastritis and improvements in vaccine efficacy are also discussed. A major area of interest is the mechanism by which immunization actually influences Helicobacter colonization. This remains a mystery: antibodies appear to be unimportant whereas CD4+ T-cells essential. Finally, a viewpoint is given on whom should be immunized when a final vaccine becomes available.

show abstract

Promotion of Ulcerative Duodenitis in Young Ferrets by Oral Immunization with Helicobacter mustelae and Muramyl Dipeptide

Cited by 22 publications

References 47 publications

Comparison of Diagnostic Techniques forHelicobacter cetorumInfection in Wild Atlantic Bottlenose Dolphins (Tursiops truncatus)

Comparison of Diagnostic Techniques forHelicobacter cetorumInfection in Wild Atlantic Bottlenose Dolphins (Tursiops truncatus)

Emergence of DiverseHelicobacterSpecies in the Pathogenesis of Gastric and Enterohepatic Diseases

Helicobacter pylori vaccines—the current status

Contact Info

Product

Resources

About