Characterization and study of the antibacterial mechanisms of silver nanoparticles prepared with microalgal exopolysaccharides

Gallón, Sandra M. Navarro; Alpaslan, Ece; Wang, Mian; Larese-Casanova, Phillip; Londoño, Martha Elena; Atehortúa, Lucía; Pavón, Juan José; Webster, Thomas J.

doi:10.1016/j.msec.2019.01.134

Cited by 92 publications

(75 citation statements)

References 54 publications

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“…Interestingly, this activity has been also demonstrated using Ag-NPs obtained by 'biological methods' which are considered a great tool to reduce the negative effects associated with traditional nanoparticle synthesis commonly used in the laboratory [13]. In particular, two recent studies have shown the antimicrobial activity of Ag-NPs from apple pomace and from exopolysaccharides isolated from green microalgae against E. coli and S. aureus [14,15].…”

Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles against Foodborne Bacteria. Effects at Intestinal Level and Health Limitations

et al. 2020

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Foodborne diseases are one of the factors that endanger the health of consumers, especially in people at risk of exclusion and in developing countries. The continuing search for effective antimicrobials to be used in the food industry has resulted in the emergence of nanotechnology in this area. Silver nanoparticles (Ag-NPs) are the nanomaterial with the best antimicrobial activity and therefore, with great potential of application in food processing and packing. However, possible health effects must be properly addressed to ensure food safety. This review presents a detailed description on the main applications of Ag-NPs as antimicrobial agents for food control, as well as the current legislation concerning these materials. Current knowledge about the impact of the dietary exposure to Ag-NPs in human health with special emphasis on the changes that nanoparticles undergo after passing through the gastrointestinal tract and how they alter the oral and gut microbiota, is also summarized. It is concluded that given their potential and wide properties against foodborne pathogens, research in Ag-NPs is of great interest but is not exempt from difficulties that must be resolved in order to certify the safety of their use.

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Section: Introductionmentioning

confidence: 99%

Silver Nanoparticles against Foodborne Bacteria. Effects at Intestinal Level and Health Limitations

et al. 2020

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“…For the mechanism of nano-silver inhibiting bacteria, there are several opinions at present. Silver nanoparticles contact with the bacterial cell wall and destroy the permeability of the bacterial cell membrane by binding with proteins and allowing intracellular substances of the bacterial cells to leak out, leading to cell death [ 19 ]. Silver nanoparticles enter bacterial cells to destroy protein and DNA structures and produce oxygen free radicals to destroy the cell structure further, causing bacterial death [ 20 ].…”

Section: Methodsmentioning

confidence: 99%

Sunlight-Induced Synthesis of Non-Target Biosafety Silver Nanoparticles for the Control of Rice Bacterial Diseases

Shang¹,

Zhou²,

Wu³

et al. 2020

Nanomaterials

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Silver is an important and efficient bactericide. Nanoscale silver has a large specific surface area, high target adhesion, strong permeability and high bactericidal activity. At present, the control of plant bacterial diseases is difficult, and the resistance of plant bacterial pathogens develops rapidly. Silver nanoparticles are expected to become a new generation of agrochemical to control plant bacterial diseases. In this study, a simple and green natural sunlight-induced method was used to prepare carboxymethylcellulose sodium-stabilized silver nanoparticles (CMC-SNs) with a particle size of around 13.53 ± 4.72 nm. CMC-SNs were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), energy-dispersive spectrometry (EDS), X-ray diffraction (XRD) and UV-vis spectroscopy and found to be spherical and evenly dispersed. The bacteriostatic activity of the CMC-SNs toward Xanthomonas oryzae pv. oryzae (Xoo) was tested. The minimum inhibitory concentration (MIC) of CMC-SNs to Xoo was 1 mg/L, and the minimum bactericidal concentration (MBC) was 2 mg/L. In addition, the antibacterial mechanism was studied by scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM), which confirmed that the CMC-SNs had high antibacterial activity. In order to verify its impact on the environment, we conducted an acute toxicity test on zebrafish and found that Half lethal concentration (LC50) > 100 mg/L in zebrafish, or no acute toxicity. The ability of CMC-SNs to control rice bacterial blight was verified by a pot experiment.

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“…The difference in efficiency may be due, among other things, to the differences in cell wall structure, which is simpler in Gram positive bacteria and more complex in Gram negative bacteria. On the other hand, the antibacterial mechanism of AgNPs is very complex as recently demonstrated [24][25][26]. Certainly, the nanostructured materials, like nano forsterite, used as carrier of AgNPs are offering new opportunities for orthopedic implant applications [27], particularly against bone infections.…”

Section: Agnps Characterizationmentioning

confidence: 99%

In-vitro Antibacterial Activity of Novel Nanostructured Composites Based on Forsterite and Silver Nanoparticles

Avram¹,

Gorea²,

Răpuntean³

et al. 2020

Rev. Chim.

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There is a continuous need for discovering new nanomaterials with antibacterial activity against various pathogens, like Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli). This study was performed to assess the antimicrobial activity of two novel nanostructured forsterites, both in the absence and the presence of silver nanoparticles (AgNPs). The two nano forsterites (FS) were prepared by advanced sol-gel (FSsg) and precipitation (FSpp) methods. Preparation of colloidal AgNPs systems was realized by using the precursor, AgNO3, and the trisodium citrate and tanic acid assuring the formation and stabilization of AgNPs. The characterization of nano forsterite powders was carried out using complementary physical methods: XRD, SEM, and AFM. The AgNPs were characterized by UV-Vis spectra, STEM and AFM imaging. The antimicrobial activity was studied by the agar well diffusion method both in the FS native state, as FSsg and FSpp, and in their mixture with silver nanoparticles (AgNPs). The inhibitory effect of synthesized forsterites, FSsg and FSpp, particularly variants with AgNPs was found only on the S. aureus strain, the zones of inhibition being between 8 and 10 mm, and more intensely expressed in the FSpp-AgNPs dispersions. These findings open new orthopedic applications of these systems, particularly for antimicrobial coated metallic implants.

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Characterization and study of the antibacterial mechanisms of silver nanoparticles prepared with microalgal exopolysaccharides

Cited by 92 publications

References 54 publications

Silver Nanoparticles against Foodborne Bacteria. Effects at Intestinal Level and Health Limitations

Silver Nanoparticles against Foodborne Bacteria. Effects at Intestinal Level and Health Limitations

Sunlight-Induced Synthesis of Non-Target Biosafety Silver Nanoparticles for the Control of Rice Bacterial Diseases

In-vitro Antibacterial Activity of Novel Nanostructured Composites Based on Forsterite and Silver Nanoparticles

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