Sunlight-Induced Synthesis of Non-Target Biosafety Silver Nanoparticles for the Control of Rice Bacterial Diseases

Abstract

Silver is an important and efficient bactericide. Nanoscale silver has a large specific surface area, high target adhesion, strong permeability and high bactericidal activity. At present, the control of plant bacterial diseases is difficult, and the resistance of plant bacterial pathogens develops rapidly. Silver nanoparticles are expected to become a new generation of agrochemical to control plant bacterial diseases. In this study, a simple and green natural sunlight-induced method was used to prepare carboxymethylcellulose sodium-stabilized silver nanoparticles (CMC-SNs) with a particle size of around 13.53 ± 4.72 nm. CMC-SNs were characterized by dynamic light scattering (DLS), transmission electron microscopy (TEM), energy-dispersive spectrometry (EDS), X-ray diffraction (XRD) and UV-vis spectroscopy and found to be spherical and evenly dispersed. The bacteriostatic activity of the CMC-SNs toward Xanthomonas oryzae pv. oryzae (Xoo) was tested. The minimum inhibitory concentration (MIC) of CMC-SNs to Xoo was 1 mg/L, and the minimum bactericidal concentration (MBC) was 2 mg/L. In addition, the antibacterial mechanism was studied by scanning electron microscope (SEM) and confocal laser scanning microscope (CLSM), which confirmed that the CMC-SNs had high antibacterial activity. In order to verify its impact on the environment, we conducted an acute toxicity test on zebrafish and found that Half lethal concentration (LC50) > 100 mg/L in zebrafish, or no acute toxicity. The ability of CMC-SNs to control rice bacterial blight was verified by a pot experiment.

Adjusting the Dose of Ag-Ion Implantation on TiN–Ag-Modified SLA-Ti Creates Different Micronanostructures: Implications on Bacteriostasis, Biocompatibility, and Osteogenesis in Dental Implants

“…Antibacterial mechanisms of nanosilver particles have been reported in several studies; however, they remain controversial. The following three antibacterial mechanisms of nanosilver particles are widely accepted: (1) nanosilver particles directly damage the outer membrane, cell wall, and cell membrane of bacteria, changing the permeability of the cell membrane and finally causing cell leakage. , (2) The presence of Ag ions and Ag particles in bacterial cells leads to the production of reactive oxygen species (ROS), which cause oxidative stress and ultimately apoptosis . (3) Silver ions can bind to thiol groups on bacterial proteins, resulting in DNA degradation and metabolic dysfunctions, such as respiratory reactions, lipid synthesis, and protein synthesis impairment .…”

Selenium nanoparticles are effective in penetrating pine and causing high oxidative damage to Bursaphelenchus xylophilus in pine wilt disease control

“…While selenite (product of selenium dioxide and water) has been identified as a highly toxic chemical, 38 the morphology of nanoparticles may show reduced toxicity. 39 In this study, in vitro cell viability tests were performed to preliminarily explore the cytotoxicity of selenium (IV) oxide and selenium (0) nanoparticles. The preosteoblast cell line (MC3T3) was used to evaluate the cell survival rate at the test concentration.…”

Biosynthesis and Characterization of Silver Nanoparticles Using Tribulus terrestris Seeds: Revealed Promising Antidiabetic Potentials

“…Studies have shown that AgNPs have strong microorganism-inhibiting properties [ 10 ] and possess free radical scavenging and anti-inflammatory properties [ 11 ]. Additionally, AgNPs have been found to have wound healing properties, anti-tumor [ 12 ], antiviral, antibacterial, and anti-angiogenic effects [ 13 ]. AgNPs have been shown to trigger apoptosis and reduce the level of matrix metalloproteinase in wounds, limiting angiogenesis [ 14 ] and vascular permeability with the help of growth factors, i.e., VEGF, interleukin (IL)-1, and advanced glycation in endothelial cells of the retina [ 15 ].…”