Characterization and structure identification of an antimicrobial peptide, hominicin, produced by Staphylococcus hominis MBBL 2–9

Kim, Pyoung Il; Sohng, Jae Kyung; Sung, Changmin; Joo, Hwang-Soo; Kim, Eun‐Mi; Yamaguchi, Tokutaro; Park, Daejoong; Kim, Byung‐Gee

doi:10.1016/j.bbrc.2010.07.024

Cited by 48 publications

(34 citation statements)

References 26 publications

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“…; Kim et al . ). Besides offering the potential for treatment of mastitis, they may offer a preventive basis for and contribute to the development of a healthy udder microbiome, also when colonizing the bovine teat skin.…”

Section: Introductionmentioning

confidence: 97%

Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens

et al. 2014

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Aims: To explore antibacterial activities of coagulase-negative staphylococci (CoNS) from teat apices of dairy cows towards mastitis-causing pathogens. Methods and Results: Of 254 CoNS, 38 displayed bacteriocin-like activity after a first screening. Seven of these strains displayed activity against at least one mastitis-related pathogen (Streptococcus uberis, Streptococcus dysgalactiae and Staphylococcus aureus). Staphylococcus chromogenes L217 displayed the strongest inhibitory effect, being active against all tested mastitis-related pathogens and most tested CoNS. Based on cation exchange and reversed-phase chromatography, in addition to N-terminal Edman degradation and PCR, the antibacterial peptide was identified as a nukacin-type bacteriocin and named nukacin L217. Although staphylococcal bacteriocins are generally found in the cell-free supernatants of liquid cultures, Staph. chromogenes L217 only led to detectable activity when grown on agar medium. Conclusions: Bacteriocin-like activities are not uncommon among CoNS from teat apices and may inhibit mastitis-causing pathogens, as found for nukacin L217 production by Staph. chromogenes L217. Significance and Impact of the Study: Nukacin L217 is the first identified bacteriocin of the species Staph. chromogenes and displays unusual production kinetics, that is, requiring surface growth of its producer. The fact that nukacins are produced by different CoNS species suggests a role in the teat skin ecosystem.

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Section: Introductionmentioning

confidence: 97%

Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens

et al. 2014

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“…To date, five such peptides (Pep5, epidermin, epicidin 280, epilancin K7, and epidermicin NI01) have been characterized from Staphylococcus epidermidis (Heidrich et al 1998; Kaletta et al 1989; Sandiford and Upton 2011; Schnell et al 1988; van de Kamp et al 1995). Among other bacteriocins, gallidermin was isolated from Staphylococcus gallinarum (Kellner et al 1988), hominicin was isolated from Staphylococcus hominis (Kim et al 2010), and nukacin ISK-1 was isolated from Staphylococcus warneri (Sashihara et al 2000). Strains of S .…”

Section: Introductionmentioning

confidence: 99%

Isolation, biochemical characterization, and cloning of a bacteriocin from the poultry-associated Staphylococcus aureus strain CH-91

Władyka

Wielebska

Wloka

et al. 2012

Appl Microbiol Biotechnol

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Staphylococcus aureus strain CH-91, isolated from a broiler chicken with atopic dermatitis, has a highly proteolytic phenotype that is correlated with the disease. We describe the isolation and biochemical and molecular characterization of the AI-type lantibiotic BacCH91 from S. aureus CH-91 culture medium. The bacteriocin was purified using a three-stage procedure comprising precipitation with ammonium sulfate, extraction with organic solvents, and reversed-phase HPLC. The BacCH91 peptide is thermostable and highly resistant to cleavage by both prokaryotic and eukaryotic peptidases. The MIC for the Gram-positive bacteria ranged from 2.5 nM for Microococcus luteus through 1.3–6.0 μM for staphylococcal strains up to more than 100 μM for Lactococcus lactis. BacCH91 was ineffective against the Gram-negative strains tested at the maximal concentration (100 μM). The amino acid sequence of BacCH91 is similar to that of epidermin and gallidermin. The encoding gene (bacCH91) occurred in two allelic variants distinguishable in the restriction fragment length polymorphism assay. Variant I, identified in S. aureus CH-91, dominated in S. aureus strains of poultry origin, although strains with variant II were also identified in this group. S. aureus strains of human origin were characterized exclusively by variant II.Electronic supplementary materialThe online version of this article (doi:10.1007/s00253-012-4578-y) contains supplementary material, which is available to authorized users.

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“…The minimal growth inhibitory concentration (MIC) was determined by a broth microdilution method (16). Briefly, 100 mL of the serially diluted PVP-I spray formulation and Siribuncha ® PVP-I solution (concentrations varied from 1.25 to 0.039 (%, m/V)) in BHI broth were added to each well of a 96 well plate.…”

Section: Antibacterial Activity Of the Pvp-i Topical Spray Formulationmentioning

confidence: 99%

Quantitative analysis of povidone-iodine thin films by X-ray photoelectron spectroscopy and their physicochemical properties

Sa¹,

Sawatdee²,

Phadoongsombut

et al. 2017

Acta Pharmaceutica

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In this study, povidone-iodine (PVP-I) has been formulated as a topical spray to produce a thin film for the controlled release of I 2 . By means of experimental design, 27 formulations containing glycerol, ethanol, PEG 400, copovidone and HFA 134a as a propellant were prepared. The pH values of all formulations were in the range of 6-7. The viscosity was within the range of 11.9-85.9 mPa s. The surface tensions were 20.3 to 24.6 mN m -1 and the contact angles were between 19.3 and 38.7°. The assays for the iodine contents were within acceptable range (80-120 %). X-ray photoelectron spectroscopy analysis revealed the ionized form of iodine was much higher than the unionized form. The MIC and MBC values of the PVP-I sprays against Staphylococcus aureus, S. epidermidis and Pseudomonas aeruginosa were higher than that of commercial PVP-I solution. The cytotoxicity study confirmed that the PVP-I spray had lower toxic effects on keratinocytes and fibroblasts compared to the commercial PVP-I solution. The formulation containing 59 % ethanol, 18 % copovidone and 12 % PEG 400 showed good antibacterial activity.Keywords: povidone-iodine, topical spray, bioactivity, cytotoxicity, X-ray photoelectron spectroscopy Human skin harbors many kinds of bacteria, including potential pathogenic bacteria such as Staphylococcus aureus, Streptococcus pyogenes, S. epidermidis, Pseudomonas aeruginosa and even some anaerobic bacteria such as Clostridium spp. that could induce tetanus. These microbes can initiate diseases such as gangrene and dermatitis, when the skin is damaged (1). At procedural and surgical sites, bacteria can create infections that lead to complex

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Characterization and structure identification of an antimicrobial peptide, hominicin, produced by Staphylococcus hominis MBBL 2–9

Cited by 48 publications

References 26 publications

Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens

Antibacterial activities of coagulase-negative staphylococci from bovine teat apex skin and their inhibitory effect on mastitis-related pathogens

Isolation, biochemical characterization, and cloning of a bacteriocin from the poultry-associated Staphylococcus aureus strain CH-91

Quantitative analysis of povidone-iodine thin films by X-ray photoelectron spectroscopy and their physicochemical properties

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