Quantitative analysis of povidone-iodine thin films by X-ray photoelectron spectroscopy and their physicochemical properties

Abstract

In this study, povidone-iodine (PVP-I) has been formulated as a topical spray to produce a thin film for the controlled release of I 2 . By means of experimental design, 27 formulations containing glycerol, ethanol, PEG 400, copovidone and HFA 134a as a propellant were prepared. The pH values of all formulations were in the range of 6-7. The viscosity was within the range of 11.9-85.9 mPa s. The surface tensions were 20.3 to 24.6 mN m -1 and the contact angles were between 19.3 and 38.7°. The assays for the iodine contents were within acceptable range (80-120 %). X-ray photoelectron spectroscopy analysis revealed the ionized form of iodine was much higher than the unionized form. The MIC and MBC values of the PVP-I sprays against Staphylococcus aureus, S. epidermidis and Pseudomonas aeruginosa were higher than that of commercial PVP-I solution. The cytotoxicity study confirmed that the PVP-I spray had lower toxic effects on keratinocytes and fibroblasts compared to the commercial PVP-I solution. The formulation containing 59 % ethanol, 18 % copovidone and 12 % PEG 400 showed good antibacterial activity.Keywords: povidone-iodine, topical spray, bioactivity, cytotoxicity, X-ray photoelectron spectroscopy Human skin harbors many kinds of bacteria, including potential pathogenic bacteria such as Staphylococcus aureus, Streptococcus pyogenes, S. epidermidis, Pseudomonas aeruginosa and even some anaerobic bacteria such as Clostridium spp. that could induce tetanus. These microbes can initiate diseases such as gangrene and dermatitis, when the skin is damaged (1). At procedural and surgical sites, bacteria can create infections that lead to complex

Antibiofilm Properties of Antiseptic Agents Used on Pseudomonas aeruginosa Isolated from Diabetic Foot Ulcers

“…We observed the same trend using PVPI. The MIC was 12,500 mg·L −1 (1:8 dilution factor), higher than the values found in previous studies [ 37 , 38 , 39 , 40 ]. However, although the values obtained were high, their dilution ratios were low, varying between 0.04% and 5% (1:2 to 1:250 dilution factor) [ 37 , 38 , 39 , 40 ].…”

“…The MIC was 12,500 mg·L −1 (1:8 dilution factor), higher than the values found in previous studies [ 37 , 38 , 39 , 40 ]. However, although the values obtained were high, their dilution ratios were low, varying between 0.04% and 5% (1:2 to 1:250 dilution factor) [ 37 , 38 , 39 , 40 ]. Moreover, we noted no difference in the MIC values of the other antiseptics compared with previous studies, suggesting that the BHI medium was not solely responsible for the results obtained with the two first antiseptics.…”

Povidone-Iodine Fails to Eradicate Chronic Suppurative Otitis Media and Demonstrates Ototoxic Risk in Mice

“…There are a variety of possibilities that could explain the dichotomy between the in vitro and in vivo efficacy. Povidone-iodine has a viscosity as high as 85.9 cP, which is similar to the value seen for olive oil (33). In contrast, fluoroquinolones have such low viscosity at baseline that methylcellulose needs to be added in to allow it to be easily administered from a dropper (34).…”

Assessment of Long Distance Chasing Photometer (NAG-ADF-300-2) by Estimating the Drug Atenolol with Povidone Iodine Via CFIA

“…100 µL as a sample volume and that passes through the measuring cell (A and B) to quench of povidone iodine molecule is shown in Scheme 1. Shows a proposed expected mechanism for the reaction of atenolol with povidone iodine [37][38][39].…”