Characterization and Separation of an Inhibitor of Viral Hemagglutination Present in Urine

Tamm, Igor; Horsfall, Frank L.

doi:10.3181/00379727-74-17825

Cited by 450 publications

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“…Thus, in canines this complex floats during centrifugation, rather than precipitating [27]. Since both retinol and retinyl esters are associated with the protein, a specific binding as observed in other binding proteins of retinoid metabolism is less probable [16].…”

Section: Discussionmentioning

confidence: 99%

Vitamin A excreted in the urine of canines is associated with a Tamm-Horsfall like protein

Schweigert¹,

Raila²,

Haebel

2002

Vet. Res.

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-Under physiological conditions canines transport vitamin A in blood plasma primarily as retinyl esters bound to lipoproteins and excrete substantial amounts of vitamin A as retinol and retinyl esters with urine. In the aqueous environment of urine, the hydrophobic vitamin A has to be associated with a protein. This vitamin A-protein complex was purified to homogeneity, prepared by preparative ultracentrifugation (density 1.21 g/mL), native polyacrylamide gel electrophoresis (PAGE) and size exclusion chromatography. The vitamin A-protein complex has a high molecular mass of > 5 000 kDa under native conditions. SDS PAGE under reduced conditions revealed a single band with a molecular mass of about 100 kDa for the protein moiety. Peptides obtained after limited proteolysis with trypsin from the 100 kDa protein were characterised by MALDI-TOF mass spectrometry and showed amino acid sequence homology to the human Tamm-Horsfall Protein (THP). This was further confirmed by a positive immunoreaction of the isolated protein with crossreacting human THP antibodies. The localisation of THP in dog kidneys was determined by using immunohistology. The reaction was strong along the entire thick ascending limb of the Henle loop and distal convoluted tubule. Our data point to the possibility that THP functions as a novel carrier for vitamin A in the urine of canines. l'environnement aqueux de l'urine, la vitamine A hydrophobe doit être associée à une protéine. Ce complexe protéine-vitamine A a été purifié et homogénéisé, préparé par ultracentrifugation (1,21 g/mL de densité), électrophorèse sur gel de polyacrylamide natif (PAGE) et chromatographie d'exclusion de taille. Le complexe protéine-vitamine A a une masse moléculaire élevée supérieure à 5 000 kDa dans les conditions natives. Le SDS PAGE effectué dans des conditions réduites a révélé une bande unique avec une masse moléculaire d'environ 100 kDa pour la partie protéique. Les peptides obtenus à partir de la protéine de 100 kDa, après protéolyse limitée avec de la trypsine, ont été caractérisés par spectrométrie de masse MALDI-TOF et ont montré une homologie de séquence en acides aminés avec la protéine Tamm-Horsfall (PTH) humaine. Ceci a été confirmé par une immuno-réaction positive entre la protéine isolée et les anticorps présentant une réaction croisée avec la PTH humaine. La localisation de la PTH dans le rein de chien a été déterminée par immunohistologie. La réaction a été forte tout le long de la section ascendante épaisse de l'anse de Henle, et du tubule contourné distal. Nos résultats suggèrent que la PTH fonctionne comme un nouveau transporteur pour la vitamine A dans l'urine des canidés.vitamine A / canidés / rein / urine / protéine Tamm-Horsfall

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Section: Discussionmentioning

confidence: 99%

Vitamin A excreted in the urine of canines is associated with a Tamm-Horsfall like protein

Schweigert¹,

Raila²,

Haebel

2002

Vet. Res.

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“…Uromodulin, also known as Tamm-Horsfall protein (THP), was first described by Tamm and Horsfall in 1950 as a urinary mucoprotein that is able to inhibit viral agglutination [1]. In 1985, Muchmore and Decker isolated an 85-kDa glycoprotein with potent immunosuppressive properties from the urine of pregnant women and named it "uromodulin" to reflect its origin in the urine and its ability to inhibit antigen-induced T-cell proliferation and monocyte cytotoxicity in vitro [2].…”

Section: Introductionmentioning

confidence: 99%

Uromodulin: old friend with new roles in health and disease

Iorember

Vehaskari

2013

Pediatr Nephrol

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“…1 Although it is the most abundant urine protein under physiological conditions, its physiological role still remains unknown. Its participation in some pathological conditions of the kidney and urinary tract has been discussed.…”

Section: Introductionmentioning

confidence: 99%

Urinary excretion of Tamm–Horsfall protein in normotensive and hypertensive elderly patients

Duława

Kokot

et al. 1998

J Hum Hypertens

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Characterization and Separation of an Inhibitor of Viral Hemagglutination Present in Urine

Cited by 450 publications

References 0 publications

Vitamin A excreted in the urine of canines is associated with a Tamm-Horsfall like protein

Vitamin A excreted in the urine of canines is associated with a Tamm-Horsfall like protein

Uromodulin: old friend with new roles in health and disease

Urinary excretion of Tamm–Horsfall protein in normotensive and hypertensive elderly patients

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