Characterization and selectivity studies of molecular imprinted membranes of Puerarin using scanning electron microscopy

Abstract

Molecular-imprinted membranes of Puerarin were prepared by phase inversion technique with acrylonitrile-acrylic acid copolymer (P (AN-co-AA)). To characterize P (AN-co-AA), ubbelohde viscometer was used to measure its viscosity-molecular weight. P (AN-co-AA) with different molecular weight was used to prepare membranes. The copolymer-dimethyl sulfoxide solution with Puerarin (PU) template was coagulated in water at various temperatures. The increase in P (AN-co-AA) molecular weight and the decrease in coagulation temperature caused an increase in PU recognition property of the resultant membrane. The PU imprinted membrane prepared with P (AN-co-AA) showed good selective ability to PU. The purity of PU increased from 56.51 to 98.41 wt%. Surface and cross-section morphology of the membranes were analyzed by using scanning electron micrograph. High-performance liquid chromatography was used for the quantification of Puerarin in isolated fraction.

Molecular imprinting science and technology: a survey of the literature for the years 2004-2011

“…Traditionally, spectroscopic methods have been invaluable for the study of fundamental aspects of the molecular imprinting process, in particular, events taking place in prepolymerization mixtures. The work of recent years has seen a continued use of NMR, IR and UV‐Vis spectroscopies for characterizing monomer‐template interactions, to the point where such studies are becoming essential aspects of MIP design, either for the screening of monomers for interaction with template or for the validation of computational design data. A recent trend is the significant increase in the number of spectroscopic studies of ligand‐MIP interactions.…”

Molecularly Imprinted Membranes

“…MIMs templated with puerarin [52] were prepared by coagulation with P(AN-co-AA). The copolymer–DMSO solution with puerarin, a recently studied plant-derived molecule having several effects on human health, was obtained at various temperatures: increasing P(AN-co-AA)’s molecular weight and decreasing the coagulation temperature (25 °C) led to an improvement in puerarin recognition by the membrane and also in the purity of puerarin, which rose from 56.51 to 98.41 wt %.…”