Characterization and rheological properties analysis of the succinoglycan produced by a high-yield mutant of Rhizobium radiobacter ATCC 19358

Gao, Hongliang; Yang, Li; Tian, Jiangtao; Huang, Lingling; Huang, Dating; Zhang, Wei; Xie, Fangrui; Niu, Yan‐Ning; Jin, Mei; Chen, Jia; Zou, Chunjing; Huang, Jing; Chang, Zhongyi; Yang, Xu-Zhao; Jiang, Deming

doi:10.1016/j.ijbiomac.2020.10.087

Cited by 20 publications

(28 citation statements)

References 60 publications

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“…The polysaccharide had glucose and galactose in an average ratio of 6.6:1.0. Similar values were found by Gao et al (2021) for succinoglycans produced by a high-yielding mutant strain (glucose:galactose molar ratio of 6.65:1.00 and 6.86:1.00). The succinoglycan monomer is an octasaccharide consisting of seven glucose residues and one galactose residue, with non-saccharide substituents such as acetate, succinate and pyruvate.…”

Section: Determination Of Monosaccharide Composition Homogeneity and ...supporting

confidence: 85%

“…Its functional properties as thickeners, gelling agents, stabilizers or emulsifiers provide high technological application potential for food processing, pharmaceuticals, medical products, cosmetics (Freitas, Alves, & Reis, 2011;Moscovici, 2015), oil extraction and nanoparticle synthesis (Halder et al, 2017). Yang et al (2021) demonstrated that their applicabilities go beyond, and that many have medicinal properties, such as prebiotics, control and reduction of blood cholesterol, ability to stimulate the immune system, and antitumor activity (Gao et al, 2021;Moscovici, 2015;Yang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

“…The rheological properties of succinoglycan solutions are determined by the chemical composition of the exopolysaccharide, mainly by the amount and type of non-saccharide substituents. The rheological behavior of succinoglycan is non-Newtonian of the pseudoplastic type (Gao et al, 2021;Kavitake et al, 2019;Ruiz et al, 2015;Simsek, Mert, Campanella, & Reuhs, 2009;Zhou et al, 2014). They have a high thickening capacity in aqueous solutions due to the high molar mass and the presence of different substituents in the chemical structure of the molecule (Gao et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Rheological and structural characterization of succinoglycan obtained by bioconversion of the agroindustrial residue deproteinized whey

Delani

Sampaio

Palacios

et al. 2022

Preprint

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Succinoglycan is a bacterial exopolysaccharide with thickening, gelling, stabilizing and emulsifying properties. In this study succinoglycan was produced from whey and its rheological and structural characteristics were elucidated. Four culture media were prepared with different ions for bioconversion of whey into succinoglycan. The formulation composed of deproteinized whey, monobasic potassium phosphate and magnesium sulfate allowed the production of 13.7 ± 0.43 g/L of succinoglycan. The rheological results showed that the apparent viscosity of the succinoglycan solutions was directly proportional to the concentration, and the solution showed pseudoplastic behavior. Dynamic frequency sweep tests identified that a concentration of 2.0% of succinoglycan is required for formation of the gel system. Temperature influenced the viscoelastic behavior of succinoglycan and revealed the melting point and reversibility of the gel. The apparent molar mass of succinoglycan was estimated to be 9.033 × 105 g/mol and the polydispersity index was 1.044, representing the homogeneity of the sample. The monosaccharide composition of glucose and galactose for the succinoglycan produced was 6.6:1.0. The 1H RMN analysis revealed the non-saccharide substituent content of 1.2%, 3.0% and 8.1% for acetyl, succinate and pyruvate, respectively. Whey was shown to be a promising carbon source for the production of succinoglycan with thickening potential and viscosity modifier.

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Section: Determination Of Monosaccharide Composition Homogeneity and ...supporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Rheological and structural characterization of succinoglycan obtained by bioconversion of the agroindustrial residue deproteinized whey

Delani

Sampaio

Palacios

et al. 2022

Preprint

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“…It is used as a drug delivery carrier for the sustained release of drugs and as a support matrix for the immobilization of enzymes ( 45 ). Rhizobium radiobacter is another Gram-negative bacterium, which can yield 31 g/L of curdlan after 72 h of fermentation ( 46 ). A recent study confirmed that curdlan obtained from R. radiobacter is highly thermostable with excellent water-holding capacity and can be used as an antioxidant and prebiotic agent.…”

Section: Biosynthesis Of Curdlanmentioning

confidence: 99%

Therapeutic and Industrial Applications of Curdlan With Overview on Its Recent Patents

et al. 2021

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Curdlan is an exopolysaccharide, which is composed of glucose linked with β-(1,3)-glycosidic bond and is produced by bacteria, such as Alcaligenes spp., Agrobacterium spp., Paenibacillus spp., Rhizobium spp., Saccharomyces cerevisiae, Candida spp., and fungal sources like Aureobasidium pullulan, Poria cocos, etc. Curdlan has been utilized in the food and pharmaceutical industries for its prebiotic, viscosifying, and water-holding properties for decades. Recently, the usefulness of curdlan has been further explored by the pharmaceutical industry for its potential therapeutic applications. Curdlan has exhibited immunoregulatory and antitumor activity in preclinical settings. It was observed that curdlan can prevent the proliferation of malarial merozoites in vivo; therefore, it may be considered as a promising therapy for the treatment of end-stage malaria. In addition, curdlan has demonstrated potent antiviral effects against human immunodeficiency virus (HIV) and Aedes aegypti virus. It has been suggested that the virucidal properties of curdlans should be extended further for other deadly viruses, such as severe acute respiratory syndrome (SARS), Middle East respiratory syndrome (MERS), and the current severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2/COVID-19). The prebiotic property of curdlan would confer beneficial effects on the host by promoting the growth of healthy microbiota in the gut and consequently help to reduce gastrointestinal disorders. Therefore, curdlan can be employed in the manufacture of prebiotics for the management of various gastrointestinal dysbiosis problems. Studies on the mechanism of action of curdlan-induced suppression in microbial and tumor cells at the cellular and molecular levels would not only enhance our understanding regarding the therapeutic effectiveness of curdlan but also help in the discovery of new drugs and dietary supplements. The primary focus of this review is to highlight the therapeutic interventions of curdlan as an anticancer, anti-malaria, antiviral, and antibacterial agent in humans. In addition, our review provides the latest information about the chemistry and biosynthesis of curdlan and its applications for making novel dairy products, functional foods, and nutraceuticals and also details about the recent patents of curdlan and its derivatives.

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“…Succinoglycan, one of the bacterial EPS, is an anionic and water-soluble extracellular polysaccharide produced by Rhizobium, Agrobacterium, and other soil bacteria. This anionic exopolysaccharide plays an important role in building an effective system of symbiotic nodules, especially with legumes [35][36][37][38][39][40][41][42], because it is highly stable in high temperature and pressure and suitable for a wide range of applications in other industries, including foods, pharmaceuticals, and thickeners, stabilizers, emulsifiers, texture treatments, and gelling agents, under optimized operating conditions, like high shear rates and extreme pH [43,44]. Table 1 shows general information regarding the physiochemical properties, and some applications of various anionic bacterial EPS.…”

Section: Introductionmentioning

confidence: 99%

Bacterial Succinoglycans: Structure, Physical Properties, and Applications

Jeong

Kim

et al. 2022

Polymers

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Succinoglycan is a type of bacterial anionic exopolysaccharide produced from Rhizobium, Agrobacterium, and other soil bacteria. The exact structure of succinoglycan depends in part on the type of bacterial strain, and the final production yield also depends on the medium composition, culture conditions, and genotype of each strain. Various bacterial polysaccharides, such as cellulose, xanthan, gellan, and pullulan, that can be mass-produced for biotechnology are being actively studied. However, in the case of succinoglycan, a bacterial polysaccharide, relatively few reports on production strains or chemical and structural characteristics have been published. Physical properties of succinoglycan, a non-Newtonian and shear thinning fluid, have been reported according to the ratio of substituents (pyruvyl, succinyl, acetyl group), molecular weight (Mw), and measurement conditions (concentration, temperature, pH, metal ion, etc.). Due to its unique rheological properties, succinoglycan has been mainly used as a thickener and emulsifier in the cosmetic and food industries. However, in recent reports, succinoglycan and its derivatives have been used as functional biomaterials, e.g., in stimuli-responsive drug delivery systems, therapeutics, and cell culture scaffolds. This suggests a new and expanded application of succinoglycan as promising biomaterials in biomedical fields, such as tissue engineering, regenerative medicine, and pharmaceuticals using drug delivery.

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Characterization and rheological properties analysis of the succinoglycan produced by a high-yield mutant of Rhizobium radiobacter ATCC 19358

Cited by 20 publications

References 60 publications

Rheological and structural characterization of succinoglycan obtained by bioconversion of the agroindustrial residue deproteinized whey

Rheological and structural characterization of succinoglycan obtained by bioconversion of the agroindustrial residue deproteinized whey

Therapeutic and Industrial Applications of Curdlan With Overview on Its Recent Patents

Bacterial Succinoglycans: Structure, Physical Properties, and Applications

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