Characterization and Quantitative Analysis of Phenylpropanoid Amides in Eggplant (Solanum melongena L.) by High Performance Liquid Chromatography Coupled with Diode Array Detection and Hybrid Ion Trap Time-of-Flight Mass Spectrometry

Abstract

Eggplant (Solanum melongena L.) is a famous edible and medicinal plant. Despite being widely cultivated and used, data on certain parts other than the fruit are limited. The present study focused on the qualitative and quantitative analysis of the chemical constituents, particularly phenylpropanoid amides (PAs), in eggplant. The mass fragmentation patterns of PAs were proposed using seven authentic compounds with the assistance of a hybrid ion trap time-of-flight mass spectrometer. Thirty-seven compounds (27 PAs and 10 others) were detected and plausibly assigned in the different parts of eggplant. Afterward, a reliable method based on liquid chromatography coupled with diode array detection was developed, validated, and applied for the simultaneous determination of seven PAs and three caffeoylquinic acids in 17 batches of eggplant roots with satisfactory accuracy, precision, and reproducibility, which could not only provide global chemical insight of eggplant but also offer a reliable tool for quality control.

Feruloyl dopamine-O-hexosides are efficient marker compounds as orthogonal validation for authentication of black cohosh (Actaea racemosa)—an UHPLC-HRAM-MS chemometrics study

“…S3) demostrated consistent fragmentation patterns. Accurate mass measurement MS and MS 2 spectra showed that the cleavage of the amide bond to eliminate the amine moiety is preferred, and produces a predominant ion corresponding to the hydroxycinnamoyl group, which is in accordance with previous reports [34, 35]. The amine moiety could be determined by the exact neutral loss value.…”

Characterization of tropane and cinnamamide alkaloids from Scopolia tangutica by high‐performance liquid chromatography with quadrupole time‐of‐flight tandem mass spectrometry

“…HPLC–MS/MS has been applied in the identification of compounds in a variety of TCMs, such as anthocyanins in Lycium ruthenicum Murray , alkaloids in Piper longum L ., phenolic amides in Cortex Lycii , etc . Additionally, the fragmentation of atropine analogs and cinnamamide alkaloids has been studied in some earlier research . Based on these works, it is feasible and practical to identify more potential alkaloids including tropanes and cinnamamide ones from S. tangutica .…”

Discovery of new muscarinic acetylcholine receptor antagonists from Scopolia tangutica

“…Furthermore, six tropane compounds and three cinnamic acid amides were discovered from S. tangutica for the first time. They are characterized as dihydroanisodamine ( 5 ), dihydroanisodine ( 6 ), noranisodine31 ( 7 ), noranisodamine ( 9 )32, deepoxyanisodine33 ( 10 ), norhyoscyamine ( 11 )3334, N-trans-p- Coumaroylputrescine3536 ( 13 ), N-trans-isoferuloylputrescine3738 ( 15 ) and N-cis- isoferuloylputrescine38 ( 16 ), based on their mass fragment and NMR spectrum. The elucidation and specific assignments of these compounds were in supporting information.…”

“…The elucidation and specific assignments of these compounds were in supporting information. Other known compounds are isolated as scopolamine3940 ( 1 ), anisodamine4142 ( 2 ), hyoscyamine4344 ( 3 ), anisodine31 ( 4 ), N-Caffeoylputrescine364546 ( 14 ), N1,N10-di-dihydrocaffeoylspermidine2147 ( 17 ), Scotanamine D21 (19), and N1-caffeoyl-N3-dihydrocaffeoyl spermidine48 ( 20 )…”