Characterization and quantification of red cell lipids in normal man

Ways, Peter; Hanahan, Donald J.

doi:10.1016/s0022-2275(20)40200-7

Cited by 438 publications

(34 citation statements)

References 36 publications

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“…Lipid analysis Sebaceous lipids were extracted using the Folch-Lees extraction method (Folch et al, 1957) as modified by Ways and Hanahan (1964) and described by Christie (1982). The lipid yields were determined gravimetrically.…”

Section: [U-14c]acetate [U-14c]glucose [U-14c]lactate [U-14c]glutamine and [U-14c]isoleucine Incorporationmentioning

confidence: 99%

Lipogenesis in the Human Sebaceous Gland: Glycogen and Glycerophosphate are Substrates for the Synthesis of Sebum Lipids

Downie

Kealey

1998

Journal of Investigative Dermatology

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The lipid class compositions of freshly isolated and overnight maintained human sebaceous glands were determined using high performance thin layer chromatography and were found to be broadly similar. The lipid classes identified in freshly isolated glands were broadly similar to those generated from various radiolabeled precursors in vitro, although lower amounts of wax/sterol esters and cholesterol were observed in vitro. We determined the glycogen content of sebaceous glands and showed that during their incubation with several radiolabeled substrates, with the exception of [U-14C]glucose, significant glycogen breakdown occurred, thus providing acetyl-CoA and NADPH that could affect the pattern of lipids synthesized. We examined glycogen-depleted glands and found that their rates and pattern of lipogenesis resemble that of nondepleted glands, except that the squalene/triacylglycerides ratio for acetate and glutamine doubled from 1.6:1 to 3.4:1 and from 0.7:1 to 1.4:1, respectively. We have shown that exogenous glycerol reduces the squalene/triacylglycerides ratio from acetate from 3.4:1 to 1.7:1, suggesting that glycogen-derived glycerophosphate is important in triacylglycerides synthesis from acetate. Moreover, glands express a glycerokinase activity that may fully account for the rate of triacylglycerides synthesis seen in vitro. We conclude that glycerophosphate generation may be as important as NADPH generation in explaining the directing effects of different substrates within the sebaceous gland.

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Section: [U-14c]acetate [U-14c]glucose [U-14c]lactate [U-14c]glutamine and [U-14c]isoleucine Incorporationmentioning

confidence: 99%

Lipogenesis in the Human Sebaceous Gland: Glycogen and Glycerophosphate are Substrates for the Synthesis of Sebum Lipids

Downie

Kealey

1998

Journal of Investigative Dermatology

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“…The lipids were extracted using a modiÐed Folch procedure. 20 The lyophilized body Ñuids [5 ml of blood plasma and 70 ml of cerebrospinal Ñuid (CSF) combined from four patients] were extracted three times with chloroformÈmethanol (2 : 1), the Ðrst time with 4.5 ml then twice with 1.5 ml. The combined extracts were washed once with 1/4 volume of water and once with 1/4 volume of waterÈmethanol (1 : 1).…”

Section: Samplesmentioning

confidence: 99%

Assignment of mono- and polyunsaturated fatty acids in lipids of tissues and body fluids

Willker

Leibfritz

1998

Magn. Reson. Chem.

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Semi-selective 800 MHz 1H, 13C HSQC NMR spectra provide ultra-high resolution of the double bond region of lipid spectra and the possibility of assigning signals to individual unsaturated fatty acid side-chains such as 18 : 1, 18 : 2 or 20 : 4 even in complex mixtures such as bioÑuids. Additional HSQC-TOCSY spectra correlate the direct double bond signals with the adjacent allylic high-Ðeld proton signals of mono-and polyunsaturated fatty acids. These signals show characteristic chemical shifts to di †erentiate unsaturated fatty acids such as 18 : 1, 18 : 2 and 20 : 4 (or others). By this means, the amount of each type of unsaturated fatty acid can be quantiÐed. Typical lipid spectra of body Ñuids (blood plasma and cerebrospinal Ñuid) and pig brain (grey and white matter) are presented and discussed with respect to the use of this technique for diagnostic purposes.

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“…Liver samples were snap-frozen and stored at −80 • C until analysis. Total lipids were extracted from liver tissue according to a modified Folch procedure as described previously [13,51]. The lipid extract was treated with 0.5 M KOH/MeOH for 20 min at room temperature, and the corresponding FA methyl esters (FAMEs) were formed and analyzed by gas chromatography (GC).…”

Section: Total Lipid Extraction and Gc Lipid Analysismentioning

confidence: 99%

Chronic High Fat Diet Intake Impairs Hepatic Metabolic Parameters in Ovariectomized Sirt3 KO Mice

Pinterić

Podgorski

Hadžija

et al. 2021

IJMS

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High fat diet (HFD) is an important factor in the development of metabolic diseases, with liver as metabolic center being highly exposed to its influence. However, the effect of HFD-induced metabolic stress with respect to ovary hormone depletion and sirtuin 3 (Sirt3) is not clear. Here we investigated the effect of Sirt3 in liver of ovariectomized and sham female mice upon 10 weeks of feeding with standard-fat diet (SFD) or HFD. Liver was examined by Folch, gas chromatography and lipid hydroperoxide analysis, histology and oil red staining, RT-PCR, Western blot, antioxidative enzyme and oxygen consumption analyses. In SFD-fed WT mice, ovariectomy increased Sirt3 and fatty acids synthesis, maintained mitochondrial function, and decreased levels of lipid hydroperoxides. Combination of ovariectomy and Sirt3 depletion reduced pparα, Scd-1 ratio, MUFA proportions, CII-driven respiration, and increased lipid damage. HFD compromised CII-driven respiration and activated peroxisomal ROS scavenging enzyme catalase in sham mice, whereas in combination with ovariectomy and Sirt3 depletion, increased body weight gain, expression of NAFLD- and oxidative stress-inducing genes, and impaired response of antioxidative system. Overall, this study provides evidence that protection against harmful effects of HFD in female mice is attributed to the combined effect of female sex hormones and Sirt3, thus contributing to preclinical research on possible sex-related therapeutic agents for metabolic syndrome and associated diseases.

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Characterization and quantification of red cell lipids in normal man

Cited by 438 publications

References 36 publications

Lipogenesis in the Human Sebaceous Gland: Glycogen and Glycerophosphate are Substrates for the Synthesis of Sebum Lipids

Lipogenesis in the Human Sebaceous Gland: Glycogen and Glycerophosphate are Substrates for the Synthesis of Sebum Lipids

Assignment of mono- and polyunsaturated fatty acids in lipids of tissues and body fluids

Chronic High Fat Diet Intake Impairs Hepatic Metabolic Parameters in Ovariectomized Sirt3 KO Mice

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