Characterization and quantification of eight water-soluble constituents in tubers of Pinellia ternata and in tea granules from the Chinese multiherb remedy Xiaochaihu-tang

Chen, Ping; Li, Chuan; Liang, Shipiao; Song, Guoqiang; Sun, Yan; Shi, Yanhong; Xu, Songlin; Zhang, Jiwen; Sheng, Shuqun; Yang, Yiming; Li, Min

doi:10.1016/j.jchromb.2006.05.028

Cited by 27 publications

(15 citation statements)

References 25 publications

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“…As part of our ongoing studies of Xiaochaihu-tang [4,5] [6]. We report the development of an LC-MS/MS-based method that can simultaneously quantify six licorice flavonoids in rat plasma.…”

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confidence: 99%

Simultaneous quantification of multiple licorice flavonoids in rat plasma

Liang

et al. 2007

J. Am. Soc. Mass Spectrom.

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Flavonoids are important naturally occurring polyphenols with antioxidant properties. In this study, we report the development of a liquid chromatography tandem mass spectrometry (LC-MS/MS)-based method capable of simultaneously quantifying multiple active licorice flavonoids (including liquiritin apioside, liquiritin, liquiritigenin, isoliquiritin apioside, isoliquiritin, and isoliquiritigenin) in plasma. Electrospray ionization was used to efficiently generate precursor deprotonated molecules of all the analytes and the [M-H] Ϫ ions were used to produce characteristic product ions for MS/MS analysis. We found that inclusion of a very low concentration of HCOONH 4 (0.01‰) in the LC mobile phase dramatically improved the detection limit for the tested flavonoids and decreased the interference by matrix effects, which have been referred to as "LC-electrolyte effects." Liquid-liquid extraction with ethyl acetate was effective for isolation of all the analytes and resulted in the lowest matrix effects of several tested sample cleanup methods. This bioanalytical method showed good linearity between 0.32 ng/mL and 1 g/mL analyte in 50-L plasma samples. The accuracy and precision at different analyte concentrations varied from 85 to 110% and from 0.8 to 8.8%, respectively. Finally, we demonstrated the applicability of this method in a pilot pharmacokinetic study of rats receiving an oral dose of Xiaochaihu-tang, an important Chinese herbal remedy for chronic hepatitis. The use of a low concentration of HCOONH 4 in the LC mobile phase could be used to improve LC-mass spectroscopy-or LC-MS/MS-based methods. (J Am Soc Mass Spectrom 2007, 18, 778 -782)

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“…As part of our ongoing studies of Xiaochaihu-tang [4,5] [6]. We report the development of an LC-MS/MS-based method that can simultaneously quantify six licorice flavonoids in rat plasma.…”

mentioning

confidence: 99%

Simultaneous quantification of multiple licorice flavonoids in rat plasma

Liang

et al. 2007

J. Am. Soc. Mass Spectrom.

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“…半夏는 오랫동안 동양에서 구토약, 진경약, 가래삭힘 약으 로써 위하수, 임신구토, 기침, 어지러움증, 두통, 급성위염, 인후통 등의 치료에 전통적으로 사용되어온 약재이다 [3][4][5][6][7] . 약 *교신저자 : 김호경, 대전 유성구 유성대로 1672 한국한의학연구원 한약자원그룹 ·Tel : 042-868-9502 ·Fax : 042-869-9434 ·E-mail : hkkim@kiom.re.kr #제1저자 : 조지은, 대전 유성구 유성대로 1672 한국한의학연구원 한약자원그룹 ·Tel : 042-868-9611 ·E-mail : je8556@kiom.re.kr ·접수：2013년 8월 15일 ·수정：2013년 9월 13일 ·채택：2013년 9월 22일 리학적으로는 자궁경부암 8) , 항경련 9) , 항궤양 10) , 구토 및 진정 작용 11,12) 에관한보고가있으며, 성분은 정유와 소량의 지방, 전 분, nicotine, 점액질, asparagine acid, glutamine, β -amino acid 등의 amino acid 과 uridinSe, β -sitosterol, choline, uracil, guanosine, adenosine 등이 함유하는 약재로 [13][14][15] 半夏를 포함한 처방들에 관해서도 여러 연구가 보고되었다 16,17) . 半夏(Pinellia Tuber)는 대한민국약전, 대한민국약전외한약 (생약)규격집에서 법으로 고시하고 있는 547종 한약재 중, 독 성이나 부작용의 원인으로 식품으로 제한하고 있는 162품목 중 하나이다.…”

Section: 서 론 1)unclassified

Content Comparative Analysis and Classification for Piniellia ternate, P. pedatisecta and Typhonium flagelliforme by HPLC-PDA analysis

Jo¹,

Lee²,

Kim³

et al. 2013

The Korea Journal of Herbology

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Objectives : A quantitative method using high performance liquid chromatography with a photodiode array detector(HPLC-PDA) was established for the quantitative analysis of the four main compound and pattern analysis to classification Piiellia ternate, P. pedatisecta and Typhonium flagelliforme. Methods : The analytical procedure for the determination of P. ternata, together with the known main compounds uracil, uridine, guanosine and adenosine was established. Optimum HPLC-PDA separation of these P. ternata was possible on Luna C18(2) column material, using water and acetonitrile as mobile phase. The method was validated according to regulatory guidelines. In addition, this assay method were analyzed for the content of four main compound in P. ternata, P. pedatisecta and T. flagelliforme and by data obtained from

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“…Its Rhizoma (PR) has been used in Traditional Chinese Medicines (TCMs) for its antiemetic, antitussive, anti-tumor, antimicrobial, anti-obesity, anticonvulsant activities and other therapeutic efficacies [1][2][3][4][5][6]. Studies have shown that the main constituents of PR are proteins, alkaloids, polysaccharides and organic acids [7][8][9][10]. Recently, it has been reported that an increasing number of polysaccharides from natural sources have excellent antioxidant activities [11].…”

Section: Introductionmentioning

confidence: 99%