Characterization and protein-adsorption behavior of deposited organic thin film onto titanium by plasma polymerization with hexamethyldisiloxane

Hayakawa, Tohru; Yoshinari, Masao; Nemoto, Kimiya

doi:10.1016/s0142-9612(03)00484-8

Cited by 107 publications

(86 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Surface modification with cold plasma is an effective way of enhancing surface wettability due to the removal of hydrocarbons and introduction of hydroxyl groups. We reported that the cold plasma treatment have changed surface biocompatibility in terms of cell attachment and protein adsorption 12,13) . It is also reported that the glow discharge plasma treatment, one kind of cold plasma treatment of titanium plates, enhanced hydrophilicity as well as the initial attachment and differentiation of osteoblast-like cells 14) .…”

Section: Introductionmentioning

confidence: 99%

Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

et al. 2015

Self Cite

View full text Add to dashboard Cite

The purpose of this study was to investigate the influence of superhydrophilic treatments of titanium on the behavior of osteoblastlike cells. Superhydrophilic specimens were prepared with sandblast and acid-etching (DW), oxygen plasma (Plasma) and ultraviolet light (UV), and were stored in distilled water for 3 days immediately after these treatments. Specimens stored in air for 3 weeks were used as a control Air group. Initial cell attachment, proliferation, alkaline phosphatase activity, and osteocalcin secretion of mouse osteoblast-like cells MC3T3-E1 were enhanced more on superhydrophilic groups than were Air specimens. On confocal laser scanning microscope images of cell morphology, the expression of actin filaments was observed on the superhydrophilic groups, whereas relatively little actin filament expression was seen on the Air surfaces on all culture periods. These results indicate that DW, Plasma, or UV treatment has potential for the creation and maintenance of superhydrophilic surfaces and the enhancement of the initial attachment, proliferation, and differentiation of osteoblast-like cells.

show abstract

Section: Introductionmentioning

confidence: 99%

Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

et al. 2015

Self Cite

View full text Add to dashboard Cite

show abstract

“…In particular, plasmapolymerized hexamethyldisiloxane (PP-HMDSO or, briefly, PP) was investigated as a coating material deposited on titanium dental implants. 10 Recently, a method for creating Ag/PP composites has been suggested as a promising route toward biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

Dental implants coated with a durable and antibacterial film

Vogel

Westphal

Salz

et al. 2015

Surface Innovations

View full text Add to dashboard Cite

Dental implants coated with a durable and antibacterial film Vogel et al. embedded between two layers of PP by means of subsequent plasma polymerization and metal evaporation steps. The double PP/Ag/PP layer can be directly deposited on titanium implants, whose in vivo biocompatibility within soft tissues has been investigated in Ref. 13 .Building up on these previous results, in this article, we investigate the performances of a similar PP/Ag/PP-layered coating material (briefly, PP+Ag) toward the adhesion of bacteria and eukaryotic cells in vitro. In particular, besides HeLa cellular models, the adhesion behavior of fibroblasts and osteoblasts will be addressed. With respect to the original coating procedure, 12,13 physical vapor deposition is used instead of evaporation to deposit the silver nanoparticle layer. The advantage of the physical vapor deposition is a better control and reproducibility of the nanoparticle distribution and a lesser degree of nanoparticle agglomeration. We test the coating layer resistance to sterilization and implantation into bone tissue (Section 3.2), optimize the plasma deposition parameters to guarantee eukaryotic adhesion (Section 3.3) and check the successful bacterial deterrence of the coating in fluorescence microscopy images (Section 3.4). Materials and methods Plasma polymerization and layer formationThe plasma polymerization and deposition of silver nanoparticles are performed in the same plasma reactor chamber. A sample holder with rotating platforms is mounted inside a 125-liter vacuum chamber. The chamber comprises an electrode of rectangular shape and a silver target situated behind a shutter system. Gas inlets for the HMDSO precursor (Wacker, Munich, Germany), argon, oxygen and hydrogen (Linde AG, Pullach, Germany) and a pumping system are connected to the chamber walls. In this study, three different coating layers are investigated: (a) A 2000-nm-thick polymer coating without silver particles, which possesses cell-repelling properties (PP2000); (b) a 100-nm-thick coating without silver particles, which promotes eukaryotic adhesion (PP100) and (c) the same coating including silver nanoparticles to inhibit bacterial growth but not eukaryotic adhesion (PP100+Ag). The coating layers are produced as follows: After decreasing the chamber pressure below 10 −4 mbar, HMDSO and oxygen are injected to a working pressure of 10 −2 to 10 −3 mbar. Applying a power of 160 W for 120 s, the plasma is ignited, and a 50-nm-thick polymer preconditioning layer is formed. The 2000-nm-thick coating is prepared at 120 W and oxygen to HMDSO gas flow ratio of 1:2. On top of the preconditioning layer, either the plasma polymerization is carried on until the desired thickness is reached (e.g. 100 nm for PP100), or an intermediate layer of silver nanoparticles is deposited by applying a power of 200 W for 60 s between the silver target and the chamber walls.With this procedure, we coat sand-blasted and etched titanium dental implant screws (11·5-mm length, 5·5-mm diameter ) as well as titaniu...

show abstract

“…Surface modification with cold plasma is an effective and economical way of enhancing the surface wettability due to by removal of hydrocarbon and introducing hydroxyl groups. Several studies have reported changes in surface biocompatibility in terms of cell attachment and protein adsorption with plasma treatment 10,13,14,16,17) . Glow discharge plasma treatment of titanium plates enhanced both hydrophilicity and initial attachment and differentiation of osteoblast-like cells 14) .…”

Section: Introductionmentioning

confidence: 99%

Change in surface properties of zirconia and initial attachment of osteoblastlike cells with hydrophilic treatment

et al. 2012

Self Cite

View full text Add to dashboard Cite

The objectives of this study were to characterize change in surface properties of tetragonal zirconia polycrystals (TZP) after hydrophilic treatment, and to determine the effect of such changes on initial attachment of osteoblast-like cells. Roughened surfaces were produced by alumina-blasting and acid-etching. Hydrophilic treatment comprised application of immediately after blasting and acid-etching (Blast/Etch), oxygen plasma (O2-Plasma), ultraviolet light (UV). Specimens stored in air were used as a control. The water contact angle was determined and surface analysis was performed using an X-ray photoelectron spectroscopy. Blast/Etch, O2-Plasma and UV specimens showed superhydrophilicity, and these hydrophilic treatments to TZP elicited a marked decrease in carbon content and an increase in hydroxyl groups. Hydrophilic treatments enhanced initial attachment of osteoblast-like cells and a change in cell morphologies. These results indicate that Blast/Etch, O2-Plasma, or UV treatment has potential in the creation and maintenance of superhydrophilic surfaces and enhancing initial attachment of osteoblast-like cells.

show abstract

Characterization and protein-adsorption behavior of deposited organic thin film onto titanium by plasma polymerization with hexamethyldisiloxane

Cited by 107 publications

References 35 publications

Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

Dental implants coated with a durable and antibacterial film

Change in surface properties of zirconia and initial attachment of osteoblastlike cells with hydrophilic treatment

Contact Info

Product

Resources

About