Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

Yamamura, Keisuke; Miura, Tadashi; Kou, I; Muramatsu, Takashi; Furusawa, Masahiro; Yoshinari, Masao

doi:10.4012/dmj.2014-076

Cited by 24 publications

(32 citation statements)

References 32 publications

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“…The 0.6 nm surface roughness substrate exhibited higher hydrophilicity than the 1.7 nm surface roughness substrate. Although previous studies have reported that high hydrophilicity aids cell proliferation 11,12 , our results showed that the 1.7 nm surface roughness substrate that has lower hydrophilicity promoted higher cell proliferation than the 0.6 nm surface roughness substrate that has no grains. Undoubtedly, wettability is affected not only by surface roughness but also by the nanostructure.…”

Section: Discussioncontrasting

confidence: 97%

Biological reaction control using topography regulation of nanostructured titanium

Shiozawa

Takeuchi

Akiba

et al. 2020

Sci Rep

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The micro-and nanosize surface topography of dental implants has been shown to affect the growth of surrounding cells. in this study, standardized and controlled periodic nanopatterns were fabricated with nanosized surface roughness on titanium substrates, and their influence on bone marrow stromal cells investigated. Cell proliferation assays revealed that the bare substrate with a 1.7 nm surface roughness has lower hydrophilicity but higher proliferation ability than that with a 0.6 nm surface roughness. further, with the latter substrate, directional cell growth was observed for line and groove patterns with a width of 100 nm and a height of 50 or 100 nm, but not for those with a height of 10 or 25 nm. With the smooth substrate, time-lapse microscopic analyses showed that more than 80% of the bone marrow cells on the line and groove pattern with a height of 100 nm grew and divided along the lines. As the nanosized grain structure controls the cell proliferation rate and the nanosized line and groove structure (50-100 nm) controls cell migration, division, and growth orientation, these standardized nanosized titanium structures can be used to elucidate the mechanisms by which surface topography regulates tissue responses to biomaterials.

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Section: Discussioncontrasting

confidence: 97%

Biological reaction control using topography regulation of nanostructured titanium

Shiozawa

Takeuchi

Akiba

et al. 2020

Sci Rep

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“…As was seen by Yamamura et al 18 the surface of UV-treated implants demonstrated significant differences in chemical properties and wettability. Additionally, photofunctionalisation induced an accelerated increase in the BIC ratios in the early healing stage.…”

Section: Discussionmentioning

confidence: 66%

Photofunctionalised Ti6Al4V implants enhance early phase osseointegration

Yamauchi

Itabashi²,

Wada

et al. 2017

Bone & Joint Research

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ObjectivesUltraviolet (UV) light-mediated photofunctionalisation is known to improve osseointegration of pure titanium (Ti). However, histological examination of titanium alloy (Ti6Al4V), which is frequently applied in orthopaedic and dental surgery, has not yet been performed. This study examined the osseointegration of photofunctionalised Ti6Al4V implants.MethodsTi and Ti6Al4V implants were treated with UV light, and the chemical composition and contact angle on the surfaces were evaluated to confirm photofunctionalisation. The implants were inserted into femurs in rats, and the rats were killed two or four weeks after the surgery. For histomorphometric analysis, both the bone–implant contact (BIC) ratio and the bone volume (BV) ratio were calculated from histological analysis and microcomputed tomography data.ResultsThe amount of carbon and the contact angle on both implants were significantly reduced after UV irradiation. The BIC ratios for both UV light-treated implants significantly increased at two weeks, but there was no significant difference at four weeks. There was no significant difference in the BV ratios between the UV light-treated and control implants at two or four weeks.ConclusionsThis study suggests that photofunctionalisation of Ti6Al4V implants, similar to that of Ti implants, may promotes osseointegration in early but not in the late phase of osseointegration.Cite this article: R. Yamauchi, T. Itabashi, K. Wada, T. Tanaka, G. Kumagai, Y. Ishibashi. Photofunctionalised Ti6Al4V implants enhance early phase osseointegration. Bone Joint Res 2017;6:331–336. DOI: 10.1302/2046-3758.65.BJR-2016-0221.R1.

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“…Sandblasted and acid-etched specimens (SB150E) of TZP were prepared by etched SB150 with 47% hydrofluoric acid (HF) for 15 min at room temperature 20) . SB150E of CpTi were prepared by etched SB150 with mixture of 36% hydrochloric acid (HCl) and 96% sulfuric acid (H 2SO4) for 3 min at 70°C 29) . Before use in cell culture experiments, all specimens were ultrasonically cleaned with acetone and distilled water for 10 min, followed by sterilization in an autoclave for 10 min at 121°C.…”

Section: Sample Preparationmentioning

confidence: 99%

Proliferation and osteogenic differentiation of human mesenchymal stem cells on zirconia and titanium with different surface topography

Hirano

Sasaki

Honma

et al. 2015

Dental Materials Journal

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The purpose of this study was to elucidate behavior of human mesenchymal stem cells (hMSCs) on yttria stabilized tetragonal zirconia polycrystals (TZP) and commercial pure titanium (CpTi) with different surface topography. Mirror-polished (MS), sandblasted with 150-μm alumina (SB150) and SB150 acid-etched (SB150E) were prepared on TZP and CpTi. Proliferation, osteogenic differentiation of hMSCs was evaluated. The scanning electron microscopy showed that micro-and nano-topographies were created on both TZP and CpTi SB150E surfaces. The proliferation ability, ALP activity, expression of Runx2 on the both SB150E specimens was significantly higher than those on the other specimens. These results suggested that creation of micro-and nano-topographies on TZP and CpTi by blast and acid-etching may offer a promising method for enhancing the proliferation and differentiation of hMSCs in clinical application.

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Influence of various superhydrophilic treatments of titanium on the initial attachment, proliferation, and differentiation of osteoblast-like cells

Cited by 24 publications

References 32 publications

Biological reaction control using topography regulation of nanostructured titanium

Biological reaction control using topography regulation of nanostructured titanium

Photofunctionalised Ti6Al4V implants enhance early phase osseointegration

Proliferation and osteogenic differentiation of human mesenchymal stem cells on zirconia and titanium with different surface topography

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