Characterization and pathogenicity of Rhizoctonia isolates collected from Brassica oleracea var. acephala in Ordu, Turkey

Türkkan, Muharrem; Kılıçoğlu, Melike Çebi; Erper, İsmail

doi:10.1007/s12600-020-00793-9

Cited by 12 publications

(12 citation statements)

References 42 publications

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“…As indicated in the previous reports, both BNR and MNR isolates cause disease in Brassica crops, including white cabbage, kale, broccoli, cauliflower, oilseed rape, and canola in several countries in the world [ 7 , 8 , 9 , 10 , 11 , 18 , 20 , 22 , 23 , 26 , 65 ]. The most prevalent and damaging R. solani AGs were AG 2-1 and AG 4 among AGs determined for brassicas [ 19 , 24 , 25 , 66 , 67 ].…”

Section: Discussionmentioning

confidence: 95%

“…Rhizoctonia spp. is an important and difficult-to-treat group among the myriad of fungal pathogens and associated with damping off, root rot, wirestem, foot rot, and the head rot disease complex of brassicas, which result in economic yield losses [ 5 , 6 , 7 , 8 , 9 , 10 , 11 ]. This phytopathogen as a complex group shows significant differences in cultural, molecular, and biochemical properties, and pathogenicity.…”

Section: Introductionmentioning

confidence: 99%

“…The isolates of MNR (AG 1 (-IB and -IC), AG 2 (-1, -2, -2IIIB, and -2IV), AG 3, AG 4 (HG-I, HG-II, and HG-III), AG 5, AG 9, AG 10), and BNR (AG-A, AG-E, AG-Fb, AG-Fc, and AG-K) have been noted to cause destructive diseases in Brassica spp. in Australia [ 19 ], Belgium [ 20 ], Canada [ 21 ], China [ 7 ], Japan [ 9 , 22 , 23 ], Brazil [ 18 ], North America [ 24 , 25 ], the United Kingdom [ 26 ], Turkey [ 10 , 11 ], and Vietnam [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…Genetic differences in hypertension and their subgroups have been phylogenetically assessed using PCR-based DNA fingerprint techniques even in the absence of prior knowledge of the sequences [ 34 , 41 , 42 , 43 , 44 ]. Several studies have reported that Rhizoctonia isolates within many AGs have caused diseases in important crops in Turkey to date [ 11 , 45 , 46 , 47 , 48 , 49 ]; however, there are no detailed reports identifying the AGs and their subgroups of Rhizoctonia isolates causing the root rot and wirestem of red cabbage in Turkey. No information is available about the aggressiveness of the red cabbage isolates to red cabbage cultivars commonly cultivated in the country.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity and Pathogenicity of Rhizoctonia spp. Isolates Associated with Red Cabbage in Samsun (Turkey)

Erper

Özer

Kalendar

et al. 2021

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A total of 132 Rhizoctonia isolates were recovered from red cabbage plants with root rot and wirestem symptoms in the province of Samsun (Turkey) between 2018 and 2019. Based on the sequence analysis of the internal transcribed spacer (ITS) region located between the 18S and 28S ribosomal RNA genes and including nuclear staining, these 124 isolates were assigned to multinucleate Rhizoctonia solani, and eight were binucleate Rhizoctonia. The most prevalent anastomosis group (AG) was AG 4 (84%), which was subdivided into AG 4 HG-I (81%) and AG 4 HG-III (3%), followed by AG 5 (10%) and AG-A (6%), respectively. The unweighted pair group method phylogenetic tree resulting from the data of 68 isolates with the inter-PBS amplification DNA profiling method based on interspersed retrotransposon element sequences confirmed the differentiation of AGs with a higher resolution. In the greenhouse experiment with representative isolates (n = 24) from AGs on red cabbage (cv. Rondale), the disease severity index was between 3.33 and 4.0 for multinucleate AG isolates and ranged from 2.5 to 3.17 for AG-A isolates. In the pathogenicity assay of six red cabbage cultivars, one isolate for each AG was tested using a similar method, and all cultivars were susceptible to AG 4 HG-I and AG 4 HG-III isolates. Redriver and Remale were moderately susceptible, while Rescue, Travero, Integro, and Rondale were susceptible to the AG 5 isolate. The results indicate that the most prevalent and aggressive AGs of Rhizoctonia are devastating pathogens to red cabbage, which means that rotation with nonhost-crops for these AGs may be the most effective control strategy. This is the first comprehensive study of Rhizoctonia isolates in red cabbage using a molecular approach to assess genetic diversity using iPBS-amplified DNA profiling.

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity and Pathogenicity of Rhizoctonia spp. Isolates Associated with Red Cabbage in Samsun (Turkey)

Erper

Özer

Kalendar

et al. 2021

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“…( Du et al, 2016 ); Brassica oleracea var. acephala ( Turkkan et al, 2020 ); Camellia sinensis ( Gao et al, 2016 ), Camptotheca acuminate ( Yang et al, 2017b ); Juglans regia ( Fan et al, 2018 ); peach ( Dissanayake et al, 2017a ); Sambucus williamsii (Caprifoliaceae) ( Yang et al, 2018a ); Schisandra chinensis (Schisandraceae) ( Yang et al, 2018a ); Senna bicapsularis ( Yang et al, 2017a ).…”

Section: Resultsmentioning

confidence: 99%

Endophytic Diaporthe Associated With Citrus grandis cv. Tomentosa in China

et al. 2021

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Diaporthe species are associated with Citrus as endophytes, pathogens, and saprobes worldwide. However, little is known about Diaporthe as endophytes in Citrus grandis in China. In this study, 24 endophytic Diaporthe isolates were obtained from cultivated C. grandis cv. “Tomentosa” in Huazhou, Guangdong Province in 2019. The nuclear ribosomal internal transcribed spacer (ITS), partial sequences of translation elongation factor 1-α (tef1), β-tubulin (tub2), and partial calmodulin (cal) gene regions were sequenced and employed to construct phylogenetic trees. Based on morphology and combined multigene phylogeny, eleven Diaporthe species were identified including two new species, Diaporthe endocitricola and D. guangdongensis. These are the first report of D. apiculata, D. aquatica, D. arecae, D. biconispora, D. limonicola, D. masirevicii, D. passifloricola, D. perseae, and D. sennae on C. grandis. This study provides the first intensive study of endophytic Diaporthe species on C. grandis cv. tomentosa in China. These results will improve the current knowledge of Diaporthe species associated with C. grandis. The results obtained in this study will also help to understand the potential pathogens and biocontrol agents and to develop a platform in disease management.

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