Characterization and optimization of mesoporous magnetic nanoparticles for immobilization and enhanced performance of porcine pancreatic lipase

Abstract

In this paper, Fe3O4 nanoparticles coated with mesoporous silica were prepared successfully, noted as Fe3O4 at the mobile composition of matter No. 41 (MCM-41). Also, Fe3O4 at MCM-41 was grafted by both 3-aminopropyltriethoxysilane (APTS) and 3-chloropropyltriethoxysilane (CPS), noted as Fe3O4 at MCM-41/APTS and Fe3O4 at MCM-41/CPS. The compounds were characterized by X-ray powder diffraction, scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, vibrating sample magnetometry, thermogravimetry and N2 adsorption/desorption. Then, the enzyme, porcine pancreas lipase (PPL), was immobilized onto these modified nanoparticles by covalent attachment, physical adsorption and cross-linking, noted as Fe3O4 at MCM-41/CPS-PPL, Fe3O4 at MCM-41-PPL and Fe3O4 at MCM-41/APTS-PPL, respectively. The results showed that Fe3O4 at MCM-41/CPS was the best nanomaterial for PPL immobilization, exhibiting enhanced immobilization efficiency (maximum 96 %), maximum relative activity (up to 96 %), high stability and reusability (83 % 56 days and 86.7 % ten cycles). Additionally, it offered some other advantages, such as easy recycling and reuse, complying with the trend of green chemistry. Therefore, Fe3O4 at MCM-41/CPS in combination with a relevant method can be proposed for commercial applications.

Graphene oxide-based Fe3O4 nanoparticles as a novel scaffold for the immobilization of porcine pancreatic lipase

“…The preparation of Fe 3 O 4 and Fe 3 O 4 @MCM-41 was carried out according to work described previously. 18 2.1.2.2 Preparation of GO. Graphene oxide (GO) was prepared using the Hummers method.…”

Synthesis, Functionalized and Characterization of Magnetic Fe3O4 Nanoparticles Protected with MCM-41 Mesoporous

“…So it is an adsorbent that competitive with Fe3O4@SiO2-NH-pydc and functionalized MCM-41 and in addition appropriate surface area, it is easily separated from analyte. If we take look at some similar synthesis of other researchers we will come to this conclusion that our syntheses have desirable properties [35,37] and functionalized by 2,6-pydc prepares it for our intended purposes.…”

“…These result in comparing the reports about Fe3O4@MCM-41, has confirmed that the functionalizing with 2,6-pydc decreased the BET surface area, pore volume and pore size. However the purposed adsorbent has better properties than the similar cases in reports [35]. TGA, DTG and DTA was used for appraising the thermal stability of synthesized Fe3O4@MCM-41-NH -2,6-pydc.…”

Functionalized Ordered Mesoporous Silicas (MCM-41): Synthesis and Applications in Catalysis

“…Jing et al [172] immobilized porcine pancrease lipase (PPL) onto magnetic MCM-41 and compared three different attachment methods. For the support preparation, Fe 3 O 4 nanoparticles were synthetized using the solvothermal method and then coated with mesoporous silica.…”