Characterization and mediation of inhibitory junction potentials from opossum lower esophageal sphincter

Conklin, Jeffrey L.; Chuang, Dun-Jie; Murray, Joseph A.; Bates, James N.

doi:10.1016/0016-5085(93)90353-e

Cited by 39 publications

(20 citation statements)

References 14 publications

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“…In various species, stimulation of intrinsic enteric nerves evokes NANC IJPs in LOS (Conklin et al, 1993;Yuan et al, 1998;Ward et al, 1999). The considerable neurotransmitters involve NO, ATP, vasoactive intestinal polypeptide and pituitary adenylate cyclase-activating polypeptide, and responsible channels suggested are apamin-sensitive K + channels and apamininsensitive channels (Rae and Muir, 1996;Imaeda et al, 1998;Selemidis et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

“…It is also the case in the LOS of several species. NO has been clarified as the important transmitter of IJP in opossum (Conklin et al, 1993), dog (De Man et al, 1991), cat (Ny et al, 1995) and guinea-pig (Imaeda et al, 1998, Yuan et al, 1998. So, NO is a potential candidate for the neurotransmitter of IJP in the murine LOS.…”

Section: Nitrergic Fast and Slow Ijpsmentioning

confidence: 99%

“…Clarification of the underlying mechanisms of the generation of IJP leads to the identification not only of the transmitter(s) but also of the channel(s) involved in these hyperpolarising responses. IJP in LOS are observed in several experimental animals, for example dog (Allescher et al, 1988), opossum (Daniel et al, 1989;Conklin et al, 1993), guineapig (Imaeda et al, 1998;Yuan et al, 1998) and mouse (Ward et al, 1998). In the guinea-pig LOS, nitric oxide (NO) and adenosine triphospahte (ATP) have been proposed as inhibitory neurotransmitters and small conductance Ca 2+ -activated K + (SKCa) channels played an important role in eliciting IJP (Imaeda et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Electrophysiological Properties of Inhibitory Junction Potential in Murine Lower Oesophageal Sphincter.

Imaeda

Cunnane

2003

J. Smooth Muscle Res.

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The electrophysiological properties of smooth muscle in the murine lower oesophageal sphincter (LOS) were investigated by intracellular microelectrode recording. Inhibitory junction potentials (IJPs) evoked by trains of field stimulation (30 V, 0.2-0.3 ms, 10 stimuli at 1-50 Hz) were observed in the murine LOS in the presence of atropine (1 µM) and nifedipine (1 µM). The IJP consists of two components, which we termed fast IJP and slow IJP. The fast IJP was partly sensitive to guanethidine (5 µM), pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS, 30 µM) and apamin (0.1 µM), suggesting that the fast IJP was produced partly through the activation of apamin- and charybdotoxin (30 nM). KT5823, a protein kinase G (PKG) inhibitor, had no effect on the fast and slow IJP in this tissue. It was suggested that, in the mouse LOS, adenosine trisphosphate (ATP) partly mediated the fast IJP through apamin-sensitive Ca 2+ -activated K + channels, and nitric oxide mediated the remained part of the fast IJP and the slow IJP through cGMP, but not PKG. ATP-sensitive K + channels were suggested to be partly involved in the production of slow IJP, but the responsible channel(s) for the nitrergic fast IJP remained unclarified.

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Section: Discussionmentioning

confidence: 99%

Section: Nitrergic Fast and Slow Ijpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Electrophysiological Properties of Inhibitory Junction Potential in Murine Lower Oesophageal Sphincter.

Imaeda

Cunnane

2003

J. Smooth Muscle Res.

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“…It has been reported that NANC inhibitory responses are mainly mediated by nitrergic nerves in esophagus from the opossum (Christinck et al, 1991;Murray et al, 1991), cat (Ny et al, 1995), cow (Barahona et al, 1998), and human (Richards et al, 1995) and also in the LES from the dog Daniel et al, 2002), opossum (Tottrup et al, 1991a,b;Conklin et al, 1993;Uc et al, 1999), guinea pig (Imaeda et al, 1998), cat (Kortezova et al, 1996), mouse (Kim et al, 1999;Sivarao et al, 2001), and human (McKirdy et al, 1992;Preiksaitis et al, 1994;Tomita et al, 1997). Biphasic, rapid and sustained, relaxations of the feline LES are suggested to be mediated by NO and VIP or VIP-like peptides (Kortezova et al, 1996), and those of the opossum sphincter appear to be mediated by NO and an unidentified substance (Uc et al, 1999).…”

Section: A Esophagusmentioning

confidence: 99%

Gastrointestinal Function Regulation by Nitrergic Efferent Nerves

Toda

Herman

2005

Pharmacol Rev

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“…NO relaxes LES and hyperpolarizes esophageal circular smooth muscle and the LES Conklin et al, 1993). Furthermore, it has also been shown that cGMP mediates the non-adrenergic non-cholinergic-induced hyperpolarization of esophageal smooth muscle (Conklin and Du, 1992).…”

Section: Discussionmentioning

confidence: 99%

The Influences of G Proteins, Ca2+, and K+ Channels on Electrical Field Stimulation in Cat Esophageal Smooth Muscle

Park

Kim

Park

et al. 2009

Korean J Physiol Pharmacol

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NO released by myenteric neurons controls the off contraction induced by electrical field stimulation (EFS) in distal esophageal smooth muscle, but in the presence of nitric oxide synthase (NOS) inhibitor, L-NAME, contraction by EFS occurs at the same time. The authors investigated the intracellular signaling pathways related with G protein and ionic channel EFS-induced contraction using cat esophageal muscles. EFS-induced contractions were significantly suppressed by tetrodotoxin (1 μM) and atropine (1 μM).

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Characterization and mediation of inhibitory junction potentials from opossum lower esophageal sphincter

Cited by 39 publications

References 14 publications

Electrophysiological Properties of Inhibitory Junction Potential in Murine Lower Oesophageal Sphincter.

Electrophysiological Properties of Inhibitory Junction Potential in Murine Lower Oesophageal Sphincter.

Gastrointestinal Function Regulation by Nitrergic Efferent Nerves

The Influences of G Proteins, Ca2+, and K+ Channels on Electrical Field Stimulation in Cat Esophageal Smooth Muscle

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